Plant Physiology Preview
Published on October 2, 2003; 10.1104/pp.103.027847
Received May 31, 2003
Returned for revision June 26, 2003
Accepted July 29, 2003
p-Chlorophenoxyisobutyric Acid Impairs Auxin Response in Arabidopsis Root
Yutaka Oono *, Chiharu Ooura , Abidur Rahman , Evalour T. Aspuria , Ken-ichiro Hayashi , Atsushi Tanaka , and Hirofumi Uchimiya
Department of Ion-beam-applied Biology (Y.O., A.R., A.T.) and Advanced Science Research Center (Y.O., C.O., E.T.A., H.U.), Japan Atomic Energy Research Institute, Takasaki 370-1292, Japan; Department of Horticulture, University of the Philippines at Los Banos, College, Laguna 4031, Philippines (E.T.A.); Department of Biochemistry, Okayama University of Science, 1-1 Ridai-cho, Okayama 700-0005, Japan (K.-I.H.); and Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan (H.U.)
* Corresponding author; email: yoono{at}taka.jaeri.go.jp.
p-Chlorophenoxyisobutyric acid (PCIB) is known as a putative antiauxin and is widely used to inhibit auxin action, although the mechanism of PCIB-mediated inhibition of auxin action is not characterized very well at the molecular level. In the present work, we showed that PCIB inhibited BA:: -glucuronidase (GUS) expression induced by indole-3-acetic acid (IAA), 2,4-dichlorophenoxyacetic acid, and 1-naphthaleneacetic acid. PCIB also inhibited auxin-dependent DR5::GUS expression. RNA hybridization and quantitative reverse transcriptase-polymerase chain reaction analyses suggested that PCIB reduced auxin-induced accumulation of transcripts of Aux/IAA genes. In addition, PCIB relieved the reduction of GUS activity in HS::AXR3NT-GUS transgenic line in which auxin inhibits GUS activity by promoting degradation of the AXR3NT-GUS fusion protein. Physiological analysis revealed that PCIB inhibited lateral root production, gravitropic response of roots, and growth of primary roots. These results suggest that PCIB impairs auxin-signaling pathway by regulating Aux/IAA protein stability and thereby affects the auxin-regulated Arabidopsis root physiology.
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