Plant Physiology Preview
Published on September 4, 2003; 10.1104/pp.103.027870
Received June 2, 2003
Returned for revision June 19, 2003
Accepted June 26, 2003
Characterization of the Targeting Signal of the Arabidopsis 22-kD Integral Peroxisomal Membrane Protein1[w]
Mary A. Murphy , Belinda A. Phillipson , Alison Baker , and Robert T. Mullen *
Department of Botany, University of Guelph, Guelph, Ontario, Canada N1G 2W1 (M.A.M., R.T.M.); and Centre for Plant Sciences, University of Leeds, Leeds, United Kingdom LS2 9JT (B.A.P., A.B.).
* Corresponding author; email: rtmullen{at}uoguelph.ca.
Using a combination of in vivo and in vitro assays, we characterized the sorting pathway and molecular targeting signal for the Arabidopsis 22-kD peroxisome membrane protein (PMP22), an integral component of the membrane of all peroxisomes in the mature plant. We show that nascent PMP22 is sorted directly from the cytosol to peroxisomes and that it is inserted into the peroxisomal boundary membrane with its N- and C-termini facing the cytosol. This direct sorting of PMP22 to peroxisomes contrasts with the indirect sorting reported previously for cottonseed (Gossypium hirsutum) ascorbate peroxidase, an integral PMP that sorts to peroxisomes via a subdomain of the endoplasmic reticulum. Thus, at least two different sorting pathways for PMPs exist in plant cells. At least four distinct regions within the N-terminal one-half of PMP22, including a positively charged domain present in most peroxisomal integral membrane-destined proteins, functions in a cooperative manner in efficient peroxisomal targeting and insertion. In addition, targeting with high fidelity to peroxisomes requires all four membrane-spanning domains in PMP22. Together, these results illustrate that the PMP22 membrane peroxisomal targeting signal is complex and that different elements within the signal may be responsible for mediating unique aspects of PMP22 biogenesis, including maintaining the solubility before membrane insertion, targeting to peroxisomes, and ensuring proper assembly in the peroxisomal boundary membrane.
This article has been cited by other articles:
|
|
|
|
 
S. M. Clark, R. Di Leo, P. K. Dhanoa, O. R. Van Cauwenberghe, R. T. Mullen, and B. J. Shelp
Biochemical characterization, mitochondrial localization, expression, and potential functions for an Arabidopsis {gamma}-aminobutyrate transaminase that utilizes both pyruvate and glyoxylate
J. Exp. Bot.,
April 1, 2009;
60(6):
1743 - 1757.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Lingard, S. K. Gidda, S. Bingham, S. J. Rothstein, R. T. Mullen, and R. N. Trelease
Arabidopsis PEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A Cooperate in Cell Cycle-Associated Replication of Peroxisomes
PLANT CELL,
June 1, 2008;
20(6):
1567 - 1585.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Liu, S. K. Ng, Y. Lu, W. Low, J. Lai, and G. Jedd
Making two organelles from one: Woronin body biogenesis by peroxisomal protein sorting
J. Cell Biol.,
January 28, 2008;
180(2):
325 - 339.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Karnik and R. N. Trelease
Arabidopsis peroxin 16 trafficks through the ER and an intermediate compartment to pre-existing peroxisomes via overlapping molecular targeting signals
J. Exp. Bot.,
May 1, 2007;
58(7):
1677 - 1693.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. W. McCartney, J. S. Greenwood, M. R. Fabian, K. A. White, and R. T. Mullen
Localization of the Tomato Bushy Stunt Virus Replication Protein p33 Reveals a Peroxisome-to-Endoplasmic Reticulum Sorting Pathway
PLANT CELL,
December 1, 2005;
17(12):
3513 - 3531.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. A. Sparkes, C. Hawes, and A. Baker
AtPEX2 and AtPEX10 Are Targeted to Peroxisomes Independently of Known Endoplasmic Reticulum Trafficking Routes
Plant Physiology,
October 1, 2005;
139(2):
690 - 700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Reumann
Specification of the Peroxisome Targeting Signals Type 1 and Type 2 of Plant Peroxisomes by Bioinformatics Analyses
Plant Physiology,
June 1, 2004;
135(2):
783 - 800.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
