Plant Physiology Preview
Published on October 9, 2003; 10.1104/pp.103.028019
Received June 3, 2003
Returned for revision July 14, 2003
Accepted August 9, 2003
SAGE Analysis of Transcriptome Responses in Arabidopsis Roots Exposed to 2,4,6-Trinitrotoluene
Drew R. Ekman , W. Walter Lorenz , Alan E. Przybyla , N. Lee Wolfe , and Jeffrey F.D. Dean *
Department of Biochemistry and Molecular Biology (D.R.E., W.W.L., A.E.P., J.F.D.D.) and Daniel B. Warnell School of Forest Resources (W.W.L., J.F.D.D.), University of Georgia, Athens, Georgia 30602; and National Exposure Research Laboratory, Ecosystems Research Division, United States Environmental Protection Agency, Athens, Georgia 30605 (N.L.W.)
* Corresponding author; email: jeffdean{at}uga.edu.
Serial analysis of gene expression was used to profile transcript levels in Arabidopsis roots and assess their responses to 2,4,6-trinitrotoluene (TNT) exposure. SAGE libraries representing control and TNT-exposed seedling root transcripts were constructed, and each was sequenced to a depth of roughly 32,000 tags. More than 19,000 unique tags were identified overall. The second most highly induced tag (27-fold increase) represented a glutathione S-transferase. Cytochrome P450 enzymes, as well as an ABC transporter and a probable nitroreductase, were highly induced by TNT exposure. Analyses also revealed an oxidative stress response upon TNT exposure. Although some increases were anticipated in light of current models for xenobiotic metabolism in plants, evidence for unsuspected conjugation pathways was also noted. Identifying transcriptome-level responses to TNT exposure will better define the metabolic pathways plants use to detoxify this xenobiotic compound, which should help improve phytoremediation strategies directed at TNT and other nitroaromatic compounds.
This article has been cited by other articles:
|
|
|
|
 
V. Srinivasasainagendra, G. P. Page, T. Mehta, I. Coulibaly, and A. E. Loraine
CressExpress: A Tool For Large-Scale Mining of Expression Data from Arabidopsis
Plant Physiology,
July 1, 2008;
147(3):
1004 - 1016.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Alferez, G. Y. Zhong, and J. K. Burns
A citrus abscission agent induces anoxia- and senescence-related gene expression in Arabidopsis
J. Exp. Bot.,
July 1, 2007;
58(10):
2451 - 2462.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Bao, S. Lee, C. Chen, X. Zhang, Y. Zhang, S. Liu, T. Clark, J. Wang, M. Cao, H. Yang, et al.
Serial Analysis of Gene Expression Study of a Hybrid Rice Strain (LYP9) and Its Parental Cultivars
Plant Physiology,
July 1, 2005;
138(3):
1216 - 1231.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Poroyko, L.G. Hejlek, W.G. Spollen, G.K. Springer, H.T. Nguyen, R.E. Sharp, and H.J. Bohnert
The Maize Root Transcriptome by Serial Analysis of Gene Expression
Plant Physiology,
July 1, 2005;
138(3):
1700 - 1710.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. P. Mezzari, K. Walters, M. Jelinkova, M.-C. Shih, C. L. Just, and J. L. Schnoor
Gene Expression and Microscopic Analysis of Arabidopsis Exposed to Chloroacetanilide Herbicides and Explosive Compounds. A Phytoremediation Approach
Plant Physiology,
June 1, 2005;
138(2):
858 - 869.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. E. Sharp, V. Poroyko, L. G. Hejlek, W. G. Spollen, G. K. Springer, H. J. Bohnert, and H. T. Nguyen
Root growth maintenance during water deficits: physiology to functional genomics
J. Exp. Bot.,
November 1, 2004;
55(407):
2343 - 2351.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Robinson, D. J. Cram, C. T. Lewis, and I. A.P. Parkin
Maximizing the Efficacy of SAGE Analysis Identifies Novel Transcripts in Arabidopsis
Plant Physiology,
October 1, 2004;
136(2):
3223 - 3233.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
