Plant Physiology Preview
Published on December 11, 2003; 10.1104/pp.103.028548
Received June 13, 2003
Returned for revision July 15, 2003
Accepted August 27, 2003
Isolated Durum Wheat and Potato Cell Mitochondria Oxidize Externally Added NADH Mostly via the Malate/Oxaloacetate Shuttle with a Rate That Depends on the Carrier-Mediated Transport
Donato Pastore , Sergio Di Pede , and Salvatore Passarella *
Dipartimento di Scienze Animali, Vegetali e dell’Ambiente, Facoltà di Agraria, Università del Molise, Via De Sanctis, 86100 Campobasso, Italy (D.P., S.D.P., S.P.)
* Corresponding author; email: passarel{at}unimol.it.
We investigated whether and how mitochondria from durum wheat (Triticum durum Desf.) and potato (Solanum tuberosum), isolated from etiolated shoots and a cell suspension culture, respectively, oxidize externally added NADH via the mitochondrial shuttles; in particular, we compared the shuttles and the external NADH dehydrogenase (NADH DHExt) with respect to their capacity to oxidize external NADH. We found that external NADH and NADPH can be oxidized via two separate DHExt, whereas under conditions in which the activities of NAD(P)H DHExt are largely prevented, NADH (but not NADPH) is oxidized in the presence of external malate (MAL) and MAL dehydrogenase, in a manner sensitive to several non-penetrant compounds according to the occurrence of the MAL/oxaloacetate (OAA) shuttle. In durum wheat mitochondria and potato cell mitochondria, the rate of NADH oxidation was limited by the rate of a novel carrier, the MAL/OAA antiporter, which is different from other carriers thought to transport OAA across the mitochondrial membrane. No NAD(P)H oxidation occurred arising from the MAL/Aspartate and the  -glycerophosphate/dihydroxyacetonphosphate shuttles. We determined the kinetic parameters of the enzymes and the antiporter involved in NADH oxidation, and, on the basis of a kinetic analysis, we showed that, at low physiological NADH concentrations, oxidation via the MAL/OAA shuttle occurred with a higher efficiency than that due to the NADH DHExt (about 100- and 10-fold at 1 µM NADH in durum wheat mitochondria and in potato cell mitochondria, respectively). The NADH DHExt contribution to NADH oxidation increased with increasing NADH concentration.
This article has been cited by other articles:
|
|
|
|
 
O. K. Atkin and D. Macherel
The crucial role of plant mitochondria in orchestrating drought tolerance
Ann. Bot.,
February 1, 2009;
103(4):
581 - 597.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. N. Laus, M. Soccio, D. Trono, L. Cattivelli, and D. Pastore
Plant Inner Membrane Anion Channel (PIMAC) Function in Plant Mitochondria
Plant Cell Physiol.,
July 1, 2008;
49(7):
1039 - 1055.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. T. Kim Hong, A. Nose, S. Agarie, and T. Yoshida
Malate metabolism in Hoya carnosa mitochondria and its role in photosynthesis during CAM phase III
J. Exp. Bot.,
May 1, 2008;
59(7):
1819 - 1827.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Pastore, D. Trono, M. N. Laus, N. Di Fonzo, and Z. Flagella
Possible plant mitochondria involvement in cell adaptation to drought stress: A case study: durum wheat mitochondria
J. Exp. Bot.,
January 1, 2007;
58(2):
195 - 210.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hisabori, S. Hara, T. Fujii, D. Yamazaki, N. Hosoya-Matsuda, and K. Motohashi
Thioredoxin affinity chromatography: a useful method for further understanding the thioredoxin network
J. Exp. Bot.,
June 1, 2005;
56(416):
1463 - 1468.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. T. K. Hong, A. Nose, and S. Agarie
Respiratory properties and malate metabolism in Percoll-purified mitochondria isolated from pineapple, Ananas comosus (L.) Merr. cv. smooth cayenne
J. Exp. Bot.,
October 1, 2004;
55(406):
2201 - 2211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Vacca, M. C. de Pinto, D. Valenti, S. Passarella, E. Marra, and L. De Gara
Production of Reactive Oxygen Species, Alteration of Cytosolic Ascorbate Peroxidase, and Impairment of Mitochondrial Metabolism Are Early Events in Heat Shock-Induced Programmed Cell Death in Tobacco Bright-Yellow 2 Cells
Plant Physiology,
March 1, 2004;
134(3):
1100 - 1112.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
