Plant Physiology Preview
Published on October 23, 2003; 10.1104/pp.103.029587
Received July 7, 2003
Returned for revision August 5, 2003
Accepted August 25, 2003
Blue Light and Phytochrome-Mediated StomatalOpening in the npq1 and phot1 phot2Mutants of Arabidopsis
Lawrence D. Talbott , Irene J. Shmayevich , Yooshun Chung , Jamila W. Hammad , and Eduardo Zeiger *
Department of Organismal Biology, Ecology, and Evolution, University of California, 900 Veteran Avenue, Los Angeles, California 90024
* Corresponding author; email: zeiger{at}biology.ucla.edu.
Recent studies have shown that blue light-specific stomatal opening is reversed by green light and that far-red light can be used to probe phytochrome-dependent stomatal movements. Here, blue-green reversibility and far-red light were used to probe the stomatal responses of the npq1 mutant and the phot1 phot2 double mutant of Arabidopsis. In plants grown at 50 µmol m-2 s-1, red light (photosynthetic)-mediated opening in isolated stomata from wild type (WT) and both mutants saturated at 100 µmol m-2 s-1. Higher fluence rates caused stomatal closing, most likely due to photo-inhibition. Blue light-specific opening, probed by adding blue light (10 µmol m-2 s-1) to a 100 µmol m-2 s-1 red background, was found in WT, but not in npq1 or phot1 phot2 double mutant stomata. Under 50 µmol m-2 s-1 red light, 10 µmol m-2 s-1 blue light opened stomata in both WT and npq1 mutant stomata but not in the phot1 phot2 double mutant. In npq1, blue light-stimulated opening was reversed by far-red but not green light, indicating that npq1 has a phytochrome-mediated response and lacks a blue light-specific response. Stomata of the phot1 phot2 double mutant opened in response to 20 to 50 µmol m-2 s-1 blue light. This opening was green light reversible and far-red light insensitive, indicating that stomata of the phot1 phot2 double mutant have a detectable blue light-specific response.
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