Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on December 11, 2003; 10.1104/pp.103.029918

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
134/1/137    most recent
pp.103.029918v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Teplitski, M.
Right arrow Articles by Bauer, W. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Teplitski, M.
Right arrow Articles by Bauer, W. D.
Agricola
Right arrow Articles by Teplitski, M.
Right arrow Articles by Bauer, W. D.

Received July 14, 2003
Returned for revision August 4, 2003
Accepted September 15, 2003

Chlamydomonas reinhardtii Secretes Compounds That Mimic Bacterial Signals and Interfere with Quorum Sensing Regulation in Bacteria

Max Teplitski , Hancai Chen , Sathish Rajamani , Mengsheng Gao , Massimo Merighi , Richard T. Sayre , Jayne B. Robinson , Barry G. Rolfe , and Wolfgang D. Bauer *

ARC Centre of Excellence for Integrative Legume Research Genomics Interaction Group, Research School of Biological Sciences, Australian National University, Canberra, Australian Capitol Territory 2601, Australia (H.C., B.G.R.); Graduate Program in Biophysics (S.R., R.T.S.) and Departments of Plant Pathology (M.M.) and Plant Biology (R.T.S.), Ohio State University, Columbus, Ohio 43210 (M.T., M.G., J.B.R); and Department of Biology, University of Dayton, Dayton Ohio 45469 (J.B.R.)

* Corresponding author; email: bauer.7{at}osu.edu.

The unicellular soil-freshwater alga Chlamydomonas reinhardtii was found to secrete substances that mimic the activity of the N-acyl-L-homoserine lactone (AHL) signal molecules used by many bacteria for quorum sensing regulation of gene expression. More than a dozen chemically separable but unidentified substances capable of specifically stimulating the LasR or CepR but not the LuxR, AhyR, or CviR AHL bacterial quorum sensing reporter strains were detected in ethyl acetate extracts of C. reinhardtii culture filtrates. Colonies of C. reinhardtii and Chlorella spp. stimulated quorum sensing-dependent luminescence in Vibrio harveyi, indicating that these algae may produce compounds that affect the AI-2 furanosyl borate diester-mediated quorum sensing system of Vibrio spp. Treatment of the soil bacterium Sinorhizobium meliloti with a partially purified LasR mimic from C. reinhardtii affected the accumulation of 16 of the 25 proteins that were altered in response to the bacterium’s own AHL signals, providing evidence that the algal mimic affected quorum sensing-regulated functions in this wild-type bacterium. Peptide mass fingerprinting identified 32 proteins affected by the bacterium’s AHLs or the purified algal mimic, including GroEL chaperonins, the nitrogen regulatory protein PII, and a GTP-binding protein. The algal mimic was able to cancel the stimulatory effects of bacterial AHLs on the accumulation of seven of these proteins, providing evidence that the secretion of AHL mimics by the alga could be effective in disruption of quorum sensing in naturally encountered bacteria.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
S.-W. Lee, S.-W. Han, L. E. Bartley, and P. C. Ronald
From the Academy: Colloquium Review: Unique characteristics of Xanthomonas oryzae pv. oryzae AvrXa21 and implications for plant innate immunity
PNAS, December 5, 2006; 103(49): 18395 - 18400.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
J. E. Gonzalez and N. D. Keshavan
Messing with Bacterial Quorum Sensing
Microbiol. Mol. Biol. Rev., December 1, 2006; 70(4): 859 - 875.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
N. D. Keshavan, P. K. Chowdhary, D. C. Haines, and J. E. Gonzalez
L-Canavanine Made by Medicago sativa Interferes with Quorum Sensing in Sinorhizobium meliloti
J. Bacteriol., December 15, 2005; 187(24): 8427 - 8436.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Gao, H. Chen, A. Eberhard, M. R. Gronquist, J. B. Robinson, B. G. Rolfe, and W. D. Bauer
sinI- and expR-Dependent Quorum Sensing in Sinorhizobium meliloti
J. Bacteriol., December 1, 2005; 187(23): 7931 - 7944.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists