Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on January 8, 2004; 10.1104/pp.103.030189

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
134/1/510    most recent
pp.103.030189v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lücker, J.
Right arrow Articles by Verhoeven, H. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lücker, J.
Right arrow Articles by Verhoeven, H. A.
Agricola
Right arrow Articles by Lücker, J.
Right arrow Articles by Verhoeven, H. A.

Received July 28, 2003
Returned for revision August 10, 2003
Accepted September 28, 2003

Increased and Altered Fragrance of Tobacco Plants after Metabolic Engineering Using Three Monoterpene Synthases from Lemon

Joost Lücker , Wilfried Schwab , Bianca van Hautum , Jan Blaas , Linus H. W. van der Plas , Harro J. Bouwmeester , and Harrie A. Verhoeven *

Business Unit Bioscience, Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands (J.L., B.v.H., J.B., H.J.B., H.A.V.); University of Würzburg, Chair of Food Chemistry, Am Hubland, 97974, Würzburg, Germany (W.S.); and Laboratory of Plant Physiology, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands (L.H.W.v.d.P.)

* Corresponding author; email: harrie.verhoeven{at}wur.nl.

Wild-type tobacco (Nicotiana tabacum) plants emit low levels of terpenoids, particularly from the flowers. By genetic modification of tobacco cv Petit Havana SR1 using three different monoterpene synthases from lemon (Citrus limon L. Burm. f.) and the subsequent combination of these three into one plant by crossings, we show that it is possible to increase the amount and alter the composition of the blend of monoterpenoids produced in tobacco plants. The transgenic tobacco plant line with the three introduced monoterpene synthases is emitting {beta}-pinene, limonene, and {gamma}-terpinene and a number of side products of the introduced monoterpene synthases, from its leaves and flowers, in addition to the terpenoids emitted by wild-type plants. The results show that there is a sufficiently high level of substrate accessible for the introduced enzymes.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. Dafny-Yelin and T. Tzfira
Delivery of Multiple Transgenes to Plant Cells
Plant Physiology, December 1, 2007; 145(4): 1118 - 1128.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Dudareva, E. Pichersky, and J. Gershenzon
Biochemistry of Plant Volatiles
Plant Physiology, August 1, 2004; 135(4): 1893 - 1902.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2004 by the American Society of Plant Biologists