Plant Physiology Preview
Published on January 15, 2004; 10.1104/pp.103.030460
Received July 17, 2003
Returned for revision August 18, 2003
Accepted October 29, 2003
Auxin from the Developing Inflorescence is Required for the Biosynthesis of Active Gibberellins in Barley Stems
Carla M. Wolbang , Peter M. Chandler , Jennifer J. Smith , and John J. Ross *
School of Plant Science, University of Tasmania, G.P.O. Box 252-55, Hobart, Tasmania 7001, Australia (C.M.W., J.J.S., J.J.R.); and Commonwealth Scientific and Industrial Research Organization Plant Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (P.M.C.)
* Corresponding author; email: John.Ross{at}utas.edu.au.
Multiple gibberellins (GAs) were quantified in the stems of intact, decapitated, and decapitated auxin-treated barley (Hordeum vulgare) plants. Removal of the developing inflorescence reduced the endogenous levels of indole-3-acetic acid (IAA), GA1, and GA3 and increased the level of GA29 in internodal and nodal tissues below the site of excision. Application of IAA to the excised stump restored GA levels to normal in almost all cases. The conversion of [14C]GA20 to bioactive [14C]GA1 and of [14C]GA5 to bioactive [14C]GA3 was reduced by decapitation, and IAA application was able to restore conversion rates back to the levels found in intact plants. The amount of mRNA for the principal vegetative 3-oxidase (converting GA20 to GA1, and GA5 to GA3) was decreased in decapitated plants and restored by IAA application. The results indicate that the inflorescence of barley is a source of IAA that is transported basipetally into the internodes and nodes where bioactive GA1 and GA3 are biosynthesized. Thus, IAA is required for normal GA biosynthesis in stems, acting at multiple steps in the latter part of the pathway.
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