Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on December 4, 2003; 10.1104/pp.103.031310

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
134/1/380    most recent
pp.103.031310v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (18)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, C. S.
Right arrow Articles by Larkins, B. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, C. S.
Right arrow Articles by Larkins, B. A.
Agricola
Right arrow Articles by Kim, C. S.
Right arrow Articles by Larkins, B. A.

Received August 4, 2003
Returned for revision October 3, 2003
Accepted October 15, 2003

A Defective Signal Peptide in a 19-kD {alpha}-Zein Protein Causes the Unfolded Protein Response and an Opaque Endosperm Phenotype in the Maize De*-B30 Mutant

Cheol Soo Kim , Brenda G. Hunter , Jeffery Kraft , Rebecca S. Boston , Sarah Yans , Rudolf Jung , and Brian A. Larkins *

Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (C.S.K., B.G.H., J.K., B.A.L.); Department of Botany, North Carolina State University, Raleigh, North Carolina 27695-7612 (R.S.B.); and Pioneer Hi-Bred International, Johnston, Iowa 50131-1004 (S.Y., R.J.)

* Corresponding author; email: Larkins{at}Ag.Arizona.edu.

Defective endosperm* (De*)-B30 is a dominant maize (Zea mays) mutation that depresses zein synthesis in the developing endosperm. The mutant kernels have an opaque, starchy phenotype, malformed zein protein bodies, and highly increased levels of binding protein and other chaperone proteins in the endosperm. Immunoblotting revealed a novel {alpha}-zein protein in De*-B30 that migrates between the 22- and 19-kD {alpha}-zein bands. Because the De*-B30 mutation maps in a cluster of 19-kD {alpha}-zein genes, we characterized cDNA clones encoding these proteins from a developing endosperm library. This led to the identification of a 19-kD {alpha}-zein cDNA in which proline replaces serine at the 15th position of the signal peptide. Although the corresponding gene does not appear to be highly expressed in De*-B30, it was found to be tightly linked with the mutant phenotype in a segregating F2 population. Furthermore, when the protein was synthesized in yeast cells, the signal peptide appeared to be less efficiently processed than when serine replaced proline. To test whether this gene is responsible for the De*-B30 mutation, transgenic maize plants expressing this sequence were created. T1 seeds originating from the transformants manifested an opaque kernel phenotype with enhanced levels of binding protein in the endosperm, similar to De*-B30. These results are consistent with the hypothesis that the De*-B30 mutation causes a defective signal peptide in a 19-kD {alpha}-zein protein.




This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
H. Yasuda, S. Hirose, T. Kawakatsu, Y. Wakasa, and F. Takaiwa
Overexpression of BiP has Inhibitory Effects on the Accumulation of Seed Storage Proteins in Endosperm Cells of Rice
Plant Cell Physiol., August 1, 2009; 50(8): 1532 - 1543.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. A. Sabelli and B. A. Larkins
The Development of Endosperm in Grasses
Plant Physiology, January 1, 2009; 149(1): 14 - 26.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Ufaz and G. Galili
Improving the Content of Essential Amino Acids in Crop Plants: Goals and Opportunities
Plant Physiology, July 1, 2008; 147(3): 954 - 961.
[Full Text] [PDF]

Home page
 Plant CellHome page
D. R. Holding, M. S. Otegui, B. Li, R. B. Meeley, T. Dam, B. G. Hunter, R. Jung, and B. A. Larkins
The Maize Floury1 Gene Encodes a Novel Endoplasmic Reticulum Protein Involved in Zein Protein Body Formation
PLANT CELL, August 1, 2007; 19(8): 2569 - 2582.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Chung, C. S. Kim, H. N. Nguyen, R. B. Meeley, and B. A. Larkins
The Maize Zmsmu2 Gene Encodes a Putative RNA-Splicing Factor That Affects Protein Synthesis and RNA Processing during Endosperm Development
Plant Physiology, June 1, 2007; 144(2): 821 - 835.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Li, T. Shimada, H. Takahashi, H. Ueda, Y. Fukao, M. Kondo, M. Nishimura, and I. Hara-Nishimura
MAIGO2 Is Involved in Exit of Seed Storage Proteins from the Endoplasmic Reticulum in Arabidopsis thaliana
PLANT CELL, December 1, 2006; 18(12): 3535 - 3547.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. E. Kirst, D. J. Meyer, B. C. Gibbon, R. Jung, and R. S. Boston
Identification and Characterization of Endoplasmic Reticulum-Associated Degradation Proteins Differentially Affected by Endoplasmic Reticulum Stress
Plant Physiology, May 1, 2005; 138(1): 218 - 231.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. L. Houston, C. Fan, Q.-Y. Xiang, J.-M. Schulze, R. Jung, and R. S. Boston
Phylogenetic Analyses Identify 10 Classes of the Protein Disulfide Isomerase Family in Plants, Including Single-Domain Protein Disulfide Isomerase-Related Proteins
Plant Physiology, February 1, 2005; 137(2): 762 - 778.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Vitale and A. Ceriotti
Protein Quality Control Mechanisms and Protein Storage in the Endoplasmic Reticulum. A Conflict of Interests?
Plant Physiology, November 1, 2004; 136(3): 3420 - 3426.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists