Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on January 15, 2004; 10.1104/pp.103.031765

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
134/2/586    most recent
pp.103.031765v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (35)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cabané, M.
Right arrow Articles by Lapierre, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cabané, M.
Right arrow Articles by Lapierre, C.
Agricola
Right arrow Articles by Cabané, M.
Right arrow Articles by Lapierre, C.

Received August 13, 2003
Returned for revision September 8, 2003
Accepted November 3, 2003

Condensed Lignins Are Synthesized in Poplar Leaves Exposed to Ozone

Mireille Cabané *, Jean-Claude Pireaux , Eric Léger , Elisabeth Weber , Pierre Dizengremel , Brigitte Pollet , and Catherine Lapierre

Unité Mixte de Recherche 1137, Institut National de la Recherche Agronomique (INRA)-Université Henri Poincaré "Ecologie et Ecophysiologie Forestière," Université Henri Poincaré-Nancy I, Boite Postale 239, F-54506 Vandoeuvre-les-Nancy cedex, France (M.C., J.-C.P., E.L., E.W., P.D.); and Laboratoire de Chimie Biologique, Unité Mixte de Recherche 206, INRA-Institut National Agronomique Paris-Grignon Institut National Agronomique, F-78850 Thiverval-Grignon, France (B.P., C.L.)

* Corresponding author; email: cabane{at}scbiol.uhp-nancy.fr.

Poplar (Populus tremula x alba) trees (clone INRA 717-1-B4) were cultivated for 1 month in phytotronic chambers with two different levels of ozone (60 and 120 nL L-1). Foliar activities of shikimate dehydrogenase (EC 1.1.1.25), phenylalanine ammonia lyase (EC 4.3.1.5), and cinnamyl alcohol dehydrogenase (CAD, EC 1.1.1.195) were compared with control levels. In addition, we examined lignin content and structure in control and ozone-fumigated leaves. Under ozone exposure, CAD activity and CAD RNA levels were found to be rapidly and strongly increased whatever the foliar developmental stage. In contrast, shikimate dehydrogenase and phenylalanine ammonia lyase activities were increased in old and midaged leaves but not in the youngest ones. The increased activities of these enzymes involved in the late or early steps of the metabolic pathway leading to lignins were associated with a higher Klason lignin content in extract-free leaves. In addition, stress lignins synthesized in response to ozone displayed a distinct structure, relative to constitutive lignins. They were found substantially enriched in carbon-carbon interunit bonds and in p-hydroxyphenylpropane units, which is reminiscent of lignins formed at early developmental stages, in compression wood, or in response to fungal elicitor. The highest changes in lignification and in enzyme activities were obtained with the highest ozone dose (120 nL L-1). These results suggest that ozone-induced lignins might contribute to the poplar tolerance to ozone because of their barrier or antioxidant effect toward reactive oxygen species.




This article has been cited by other articles:


Home page
 Crop Sci.Home page
C. Riboulet, S. Guillaumie, V. Mechin, M. Bosio, M. Pichon, D. Goffner, C. Lapierre, B. Pollet, B. Lefevre, J. P. Martinant, et al.
Kinetics of Phenylpropanoid Gene Expression in Maize Growing Internodes: Relationships with Cell Wall Deposition
Crop Sci., January 28, 2009; 49(1): 211 - 223.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2004 by the American Society of Plant Biologists