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Published on June 1, 2004; 10.1104/pp.103.032631

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Received August 31, 2003
Returned for revision March 10, 2004
Accepted March 10, 2004

Plastidial {alpha}-Glucan Phosphorylase Is Not Required for Starch Degradation in Arabidopsis Leaves But Has a Role in the Tolerance of Abiotic Stress

Samuel C. Zeeman *, David Thorneycroft , Nicole Schupp , Andrew Chapple , Melanie Weck , Hannah Dunstan , Pierre Haldimann , Nicole Bechtold , Alison M. Smith , and Steven M. Smith

Institute of Plant Sciences, University of Bern, CH-3013 Bern, Switzerland
Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JH, United Kingdom
John Innes Centre, Norwich NR4 7UH, United Kingdom
Institut National de la Recherche Agronomique, Lab Génétique et Amélioration des Plantes, F-78026 Versailles, France

* Corresponding author; email: sam.zeeman{at}ips.unibe.ch.

To study the role of the plastidial {alpha}-glucan phosphorylase in starch metabolism in the leaves of Arabidopsis, two independent mutant lines containing T-DNA insertions within the phosphorylase gene were identified. Both insertions eliminate the activity of the plastidial {alpha}-glucan phosphorylase. Measurement of other enzymes of starch metabolism reveals only minor changes compared with the wild type. The loss of plastidial {alpha}-glucan phosphorylase does not cause a significant change in the total accumulation of starch during the day or its remobilization at night. Starch structure and composition are unaltered. However, mutant plants display lesions on their leaves that are not seen on wild-type plants, and mesophyll cells bordering the lesions accumulate high levels of starch. Lesion formation is abolished by growing plants under 100% humidity in still air, but subsequent transfer to circulating air with lower humidity causes extensive wilting in the mutant leaves. Wilted sectors die, causing large lesions that are bordered by starch-accumulating cells. Similar lesions are caused by the application of acute salt stress to mature plants. We conclude that plastidial phosphorylase is not required for the degradation of starch, but that it plays a role in the capacity of the leaf lamina to endure a transient water deficit.




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