Plant Physiology Preview
Published on December 4, 2003; 10.1104/pp.103.033480
Received September 16, 2003
Returned for revision October 10, 2003
Accepted October 20, 2003
A Methyl Viologen-Resistant Mutant of Arabidopsis, Which Is Allelic to Ozone-Sensitive rcd1, Is Tolerant to Supplemental Ultraviolet-B Irradiation
Takahiro Fujibe , Hikaru Saji , Keita Arakawa , Naoto Yabe , Yuichi Takeuchi , and Kotaro T. Yamamoto *
Division of Biological Sciences, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, 060-0810 Japan (T.F., K.T.Y.); Environmental Biology Division, National Institute for Environmental Studies, Tsukuba, 305-0053 Japan (H.S.); Low Temperature Institute, Hokkaido University, Sapporo, 060-0819 Japan (K.A.); Kihara Institute for Biological Research, Graduate School of Integrated Science, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, Yokohama, 244-0813 Japan (N.Y.); and Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University, Sapporo, 005-8601 Japan (Y.T.)
* Corresponding author; email: kty{at}sci.hokudai.ac.jp.
To better understand the role of active oxygen species (AOS) in acquired resistance to increased levels of ultraviolet (UV)-B irradiation in plants, we isolated an Arabidopsis mutant that is resistant to methyl viologen, and its sensitivity to UV-B was investigated. A complementation test revealed that the obtained mutant was allelic to the ozone-sensitive radical-induced cell death1-1 (rcd1-1). Therefore, this mutant was named rcd1-2. rcd1-2 was recessive and nearly 4-fold more resistant to methyl viologen than wild type. It exhibited a higher tolerance to short-term UV-B supplementation treatments than the wild type: UV-B-induced formation of cyclobutane pyrimidine dimers was reduced by one-half after 24 h of exposure; the decrease in quantum yield of photosystem II was also diminished by 40% after 12 h of treatment. Furthermore, rcd1-2 was tolerant to freezing. Steady-state mRNA levels of plastidic Cu/Zn superoxide dismutase and stromal ascorbate peroxidase were higher in rcd1-2 than in wild type, and the mRNA level of the latter enzyme was enhanced by UV-B exposure more effectively in rcd1-2. UV-B-absorbing compounds were more accumulated in rcd1-2 than in wild type after UV-B exposure for 24 h. These findings suggest that rcd1-2 methyl viologen resistance is due to the enhanced activities of the AOS-scavenging enzymes in chloroplasts and that the acquired tolerance to the short-term UV-B exposure results from a higher accumulation of sunscreen pigments. rcd1 appears to be a mutant that constitutively shows stress responses, leading to accumulation of more pigments and AOS-scavenging enzymes without any stresses.
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