Plant Physiology Preview
Published on February 19, 2004; 10.1104/pp.103.033936
Received September 26, 2003
Returned for revision November 18, 2003
Accepted December 9, 2003
myo-Inositol Oxygenase Offers a Possible Entry Point into Plant Ascorbate Biosynthesis
Argelia Lorence , Boris I. Chevone , Pedro Mendes , and Craig L. Nessler *
Department of Plant Pathology, Physiology, and Weed Science (A.L., B.I.C., C.L.N.) and Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 (P.M.)
* Corresponding author; email: cnessler{at}vt.edu.
Two biosynthetic pathways for ascorbate (L-ascorbic acid [AsA]; vitamin C) in plants are presently known, the mannose/L-galactose pathway and an L-GalUA pathway. Here, we present molecular and biochemical evidence for a possible biosynthetic route using myo-inositol (MI) as the initial substrate. A MI oxygenase (MIOX) gene was identified in chromosome 4 (miox4) of Arabidopsis ecotype Columbia, and its enzymatic activity was confirmed in bacterially expressed recombinant protein. Miox4 was primarily expressed in flowers and leaves of wild-type Arabidopsis plants, tissues with a high concentration of AsA. Ascorbate levels increased 2- to 3-fold in homozygous Arabidopsis lines overexpressing the miox4 open reading frame, thus suggesting the role of MI in AsA biosynthesis and the potential for using this gene for the agronomic and nutritional enhancement of crops.
This article has been cited by other articles:
|
|
|
|
 
J. Torabinejad, J. L. Donahue, B. N. Gunesekera, M. J. Allen-Daniels, and G. E. Gillaspy
VTC4 Is a Bifunctional Enzyme That Affects Myoinositol and Ascorbate Biosynthesis in Plants
Plant Physiology,
June 1, 2009;
150(2):
951 - 961.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Aluru, J. Zola, A. Foudree, and S. R. Rodermel
Chloroplast Photooxidation-Induced Transcriptome Reprogramming in Arabidopsis immutans White Leaf Sectors
Plant Physiology,
June 1, 2009;
150(2):
904 - 923.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Zhang, A. Lorence, H. A. Gruszewski, B. I. Chevone, and C. L. Nessler
AMR1, an Arabidopsis Gene That Coordinately and Negatively Regulates the Mannose/L-Galactose Ascorbic Acid Biosynthetic Pathway
Plant Physiology,
June 1, 2009;
150(2):
942 - 950.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-H. Shen, R. Krishnamurthy, and K.-W. Yeh
Decreased L-Ascorbate Content Mediating Bolting is Mainly Regulated by the Galacturonate Pathway in Oncidium
Plant Cell Physiol.,
May 1, 2009;
50(5):
935 - 946.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Bulley, M. Rassam, D. Hoser, W. Otto, N. Schunemann, M. Wright, E. MacRae, A. Gleave, and W. Laing
Gene expression studies in kiwifruit and gene over-expression in Arabidopsis indicates that GDP-L-galactose guanyltransferase is a major control point of vitamin C biosynthesis
J. Exp. Bot.,
March 1, 2009;
60(3):
765 - 778.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Ioannidi, M. S. Kalamaki, C. Engineer, I. Pateraki, D. Alexandrou, I. Mellidou, J. Giovannonni, and A. K. Kanellis
Expression profiling of ascorbic acid-related genes during tomato fruit development and ripening and in response to stress conditions
J. Exp. Bot.,
February 1, 2009;
60(2):
663 - 678.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Endres and R. Tenhaken
Myoinositol Oxygenase Controls the Level of Myoinositol in Arabidopsis, But Does Not Increase Ascorbic Acid
Plant Physiology,
February 1, 2009;
149(2):
1042 - 1049.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ishikawa, H. Nishikawa, Y. Gao, Y. Sawa, H. Shibata, Y. Yabuta, T. Maruta, and S. Shigeoka
The Pathway via D-Galacturonate/L-Galactonate Is Significant for Ascorbate Biosynthesis in Euglena gracilis: IDENTIFICATION AND FUNCTIONAL CHARACTERIZATION OF ALDONOLACTONASE
J. Biol. Chem.,
November 7, 2008;
283(45):
31133 - 31141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Maruta, M. Yonemitsu, Y. Yabuta, M. Tamoi, T. Ishikawa, and S. Shigeoka
Arabidopsis Phosphomannose Isomerase 1, but Not Phosphomannose Isomerase 2, Is Essential for Ascorbic Acid Biosynthesis
J. Biol. Chem.,
October 24, 2008;
283(43):
28842 - 28851.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Catusse, J.-M. Strub, C. Job, A. Van Dorsselaer, and D. Job
Proteome-wide characterization of sugarbeet seed vigor and its tissue specific expression
PNAS,
July 22, 2008;
105(29):
10262 - 10267.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Schneider, D. Beyhl, R. Hedrich, and N. Sauer
Functional and Physiological Characterization of Arabidopsis INOSITOL TRANSPORTER1, a Novel Tonoplast-Localized Transporter for myo-Inositol
PLANT CELL,
April 1, 2008;
20(4):
1073 - 1087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Zhang, H. A. Gruszewski, B. I. Chevone, and C. L. Nessler
An Arabidopsis Purple Acid Phosphatase with Phytase Activity Increases Foliar Ascorbate
Plant Physiology,
February 1, 2008;
146(2):
431 - 440.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Alhagdow, F. Mounet, L. Gilbert, A. Nunes-Nesi, V. Garcia, D. Just, J. Petit, B. Beauvoit, A. R. Fernie, C. Rothan, et al.
Silencing of the Mitochondrial Ascorbate Synthesizing Enzyme L-Galactono-1,4-Lactone Dehydrogenase Affects Plant and Fruit Development in Tomato
Plant Physiology,
December 1, 2007;
145(4):
1408 - 1422.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Schneider, A. Schneidereit, P. Udvardi, U. Hammes, M. Gramann, P. Dietrich, and N. Sauer
Arabidopsis INOSITOL TRANSPORTER2 Mediates H+ Symport of Different Inositol Epimers and Derivatives across the Plasma Membrane
Plant Physiology,
December 1, 2007;
145(4):
1395 - 1407.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-C. Leple, R. Dauwe, K. Morreel, V. Storme, C. Lapierre, B. Pollet, A. Naumann, K.-Y. Kang, H. Kim, K. Ruel, et al.
Downregulation of Cinnamoyl-Coenzyme A Reductase in Poplar: Multiple-Level Phenotyping Reveals Effects on Cell Wall Polymer Metabolism and Structure
PLANT CELL,
November 1, 2007;
19(11):
3669 - 3691.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Chiera and E. Grabau
Localization of myo-inositol phosphate synthase (GmMIPS-1) during the early stages of soybean seed development
J. Exp. Bot.,
June 1, 2007;
58(8):
2261 - 2268.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. A. Laing, M. A. Wright, J. Cooney, and S. M. Bulley
From the Cover: The missing step of the L-galactose pathway of ascorbate biosynthesis in plants, an L-galactose guanyltransferase, increases leaf ascorbate content
PNAS,
May 29, 2007;
104(22):
9534 - 9539.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Giovannoni
Completing a pathway to plant vitamin C synthesis
PNAS,
May 29, 2007;
104(22):
9109 - 9110.
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Stevens, M. Buret, P. Duffe, C. Garchery, P. Baldet, C. Rothan, and M. Causse
Candidate Genes and Quantitative Trait Loci Affecting Fruit Ascorbic Acid Content in Three Tomato Populations
Plant Physiology,
April 1, 2007;
143(4):
1943 - 1953.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Carrari, C. Baxter, B. Usadel, E. Urbanczyk-Wochniak, M.-I. Zanor, A. Nunes-Nesi, V. Nikiforova, D. Centero, A. Ratzka, M. Pauly, et al.
Integrated Analysis of Metabolite and Transcript Levels Reveals the Metabolic Shifts That Underlie Tomato Fruit Development and Highlight Regulatory Aspects of Metabolic Network Behavior
Plant Physiology,
December 1, 2006;
142(4):
1380 - 1396.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Matamoros, J. Loscos, M. J. Coronado, J. Ramos, S. Sato, P. S. Testillano, S. Tabata, and M. Becana
Biosynthesis of Ascorbic Acid in Legume Root Nodules
Plant Physiology,
July 1, 2006;
141(3):
1068 - 1077.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. L. Conklin, S. Gatzek, G. L. Wheeler, J. Dowdle, M. J. Raymond, S. Rolinski, M. Isupov, J. A. Littlechild, and N. Smirnoff
Arabidopsis thaliana VTC4 Encodes L-Galactose-1-P Phosphatase, a Plant Ascorbic Acid Biosynthetic Enzyme
J. Biol. Chem.,
June 9, 2006;
281(23):
15662 - 15670.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Schneider, A. Schneidereit, K. R. Konrad, M.-R. Hajirezaei, M. Gramann, R. Hedrich, and N. Sauer
Arabidopsis INOSITOL TRANSPORTER4 Mediates High-Affinity H+ Symport of Myoinositol across the Plasma Membrane
Plant Physiology,
June 1, 2006;
141(2):
565 - 577.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ghosh Dastidar, S. Maitra, L. Goswami, D. Roy, K. P. Das, and A. L. Majumder
An Insight into the Molecular Basis of Salt Tolerance of L-myo-Inositol 1-P Synthase (PcINO1) from Porteresia coarctata (Roxb.) Tateoka, a Halophytic Wild Rice
Plant Physiology,
April 1, 2006;
140(4):
1279 - 1296.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Wolucka, A. Goossens, and D. Inze
Methyl jasmonate stimulates the de novo biosynthesis of vitamin C in plant cell suspensions
J. Exp. Bot.,
September 1, 2005;
56(419):
2527 - 2538.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. R. Wilkinson, S. R. Prathalingam, M. C. Taylor, D. Horn, and J. M. Kelly
Vitamin C biosynthesis in trypanosomes: A role for the glycosome
PNAS,
August 16, 2005;
102(33):
11645 - 11650.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. A. Laing, S. Bulley, M. Wright, J. Cooney, D. Jensen, D. Barraclough, and E. MacRae
A highly specific L-galactose-1-phosphate phosphatase on the path to ascorbate biosynthesis
PNAS,
November 30, 2004;
101(48):
16976 - 16981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
