Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on April 2, 2004; 10.1104/pp.103.037754

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
134/4/1471    most recent
pp.103.037754v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Domonkos, I.
Right arrow Articles by Gombos, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Domonkos, I.
Right arrow Articles by Gombos, Z.
Agricola
Right arrow Articles by Domonkos, I.
Right arrow Articles by Gombos, Z.

Received December 15, 2003
Returned for revision January 27, 2004
Accepted January 27, 2004

Phosphatidylglycerol Is Essential for Oligomerization of Photosystem I Reaction Center

Ildikó Domonkos *, Przemyslaw Malec , Anna Sallai , László Kovács , Kunihiro Itoh , Gaozhong Shen , Bettina Ughy , Balázs Bogos , Isamu Sakurai , Mihály Kis , Kazimierz Strzalka , Hajime Wada , Shigeru Itoh , Tibor Farkas , and Zoltán Gombos

Institute of Plant Biology, Biological Research Center of the Hungarian Academy of Sciences, H-6701 Szeged, Hungary
Department of Plant Physiology and Biochemistry, Faculty of Biotechnology, Jagiellonian University, PL-30-387 Krakow, Poland
Division of Material Science (Physics), Graduate School of Science, Nagoya University, Furocho, Chikusa, Nagoya 464-8601, Japan
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komamba, Tokyo 153-8902, Japan
Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, H-6701 Szeged, Hungary (T.F.)

* Corresponding author; email: domonkos{at}nucleus.szbk.u-szeged.hu.

Our earlier studies with the pgsA mutant of Synechocystis PCC6803 demonstrated the important role of phosphatidylglycerol (PG) in PSII dimer formation and in electron transport between the primary and secondary electron-accepting plastoquinones of PSII. Using a long-term depletion of PG from pgsA mutant cells, we could induce a decrease not only in PSII but also in PSI activity. Simultaneously with the decrease in PSI activity, dramatic structural changes of the PSI complex were detected. A 21-d PG depletion resulted in the degradation of PSI trimers and concomitant accumulation of monomer PSI. The analyses of PSI particles isolated by MonoQ chromatography showed that, following the 21-d depletion, PSI trimers were no longer detectable in the thylakoid membranes. Immunoblot analyses revealed that the PSI monomers accumulating in the PG-depleted mutant cells do not contain PsaL, the protein subunit thought to be responsible for the trimer formation. Nevertheless, the trimeric structure of PSI reaction center could be restored by readdition of PG, even in the presence of the protein synthesis inhibitor lincomycin, indicating that free PsaL was present in thylakoid membranes following the 21-d PG depletion. Our data suggest an indispensable role for PG in the PsaL-mediated assembly of the PSI reaction center.




This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
I. Domonkos, P. Malec, H. Laczko-Dobos, O. Sozer, K. Klodawska, H. Wada, K. Strzalka, and Z. Gombos
Phosphatidylglycerol Depletion Induces an Increase in Myxoxanthophyll Biosynthetic Activity in Synechocystis PCC6803 Cells
Plant Cell Physiol., February 1, 2009; 50(2): 374 - 382.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
I. Sakurai, J.-R. Shen, J. Leng, S. Ohashi, M. Kobayashi, and H. Wada
Lipids in Oxygen-Evolving Photosystem II Complexes of Cyanobacteria and Higher Plants
J. Biochem., August 1, 2006; 140(2): 201 - 209.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. G. Ivanov, M. Krol, D. Sveshnikov, E. Selstam, S. Sandstrom, M. Koochek, Y.-I. Park, S. Vasil'ev, D. Bruce, G. Oquist, et al.
Iron Deficiency in Cyanobacteria Causes Monomerization of Photosystem I Trimers and Reduces the Capacity for State Transitions and the Effective Absorption Cross Section of Photosystem I in Vivo
Plant Physiology, August 1, 2006; 141(4): 1436 - 1445.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Wu, Z. Yang, and T. Kuang
Impaired Photosynthesis in Phosphatidylglycerol-Deficient Mutant of Cyanobacterium Anabaena sp. PCC7120 with a Disrupted Gene Encoding a Putative Phosphatidylglycerophosphatase
Plant Physiology, August 1, 2006; 141(4): 1274 - 1283.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2004 by the American Society of Plant Biologists