Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Published on April 30, 2004; 10.1104/pp.103.037895

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Received December 23, 2004
Returned for revision February 20, 2004
Accepted March 8, 2004

Copper Transport Across Pea Thylakoid Membranes

Richard Shingles *, Larry E. Wimmers , and Richard E. McCarty

Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218-2685

* Corresponding author; email: shingles{at}jhu.edu.

The initial rate of Cu2+ movement across the thylakoid membrane of pea (Pisum sativum) chloroplasts was directly measured by stopped-flow spectrofluorometry using membranes loaded with the Cu2+-sensitive fluorophore Phen Green SK. Cu2+ transport was rapid, reaching completion within 0.5 s. The initial rate of uptake was dependent upon Cu2+ concentration and saturated at about 0.6 µM total Cu2+. Cu2+ uptake was maximal at a thylakoid lumen pH of 7.0. Cu2+ transport was inhibited by Zn2+ but was largely unaffected by Mn2+ and Cu+. Zn2+ inhibited Cu2+ transport to a maximum of 60%, indicating that there may be more than one transporter for copper in pea thylakoid membranes.




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