Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on April 2, 2004; 10.1104/pp.103.038547

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
134/4/1775    most recent
pp.103.038547v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wei, H.
Right arrow Articles by Layzell, D. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wei, H.
Right arrow Articles by Layzell, D. B.
Agricola
Right arrow Articles by Wei, H.
Right arrow Articles by Layzell, D. B.

Received January 2, 2004
Returned for revision February 3, 2004
Accepted February 3, 2004

Adenylate Gradients and Ar:O2 Effects on Legume Nodules. II. Changes in the Subcellular Adenylate Pools

Hui Wei , Craig A. Atkins , and David B. Layzell *

Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6
Botany, School of Plant Biology, The University of Western Australia, Nedlands, Western Australia 6907, Australia (C.A.A.)

* Corresponding author; email: layzelld{at}biology.queensu.ca.

Central infected zone tissue of soybean (Glycine max L. Merr.) nodules was fractionated into separate subcellular compartments using density gradient centrifugation in nonaqueous solvents to better understand how exposure to Ar:O2 (80:20%, v/v) atmosphere affects C and N metabolism, and to explore a potential role for adenylates in regulating O2 diffusion. When nodules were switched from air to Ar:O2, adenylate energy charge (AEC) in the plant cytosol rose from 0.63 {mp} 0.02 to 0.73 {mp} 0.02 within 7 min and to 0.80 {mp} 0.01 by 60 min. In contrast, AEC of the mitochondrial compartment of this central zone tissue remained high (0.80 {mp} 0.02 to 0.81 {mp} 0.02) following Ar treatment while that of the bacteroid compartment was unchanged, at 0.73 {mp} 0.02, after 7 min, but declined to 0.57 {mp} 0.03 after 60 min. These results were consistent with a simulation model that predicted Ar:O2 exposure would first reduce ATP demand for ammonia assimilation and rapidly increase cytosolic AEC, before the Ar:O2-induced decline mediated by a decrease in nodule O2 permeability reduces bacteroid AEC. The possibility that adenylates play a key, integrating role in regulating nodule permeability to oxygen diffusion is discussed.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
H. Wei and D. B. Layzell
Adenylate-Coupled Ion Movement. A Mechanism for the Control of Nodule Permeability to O2 Diffusion
Plant Physiology, May 1, 2006; 141(1): 280 - 287.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2004 by the American Society of Plant Biologists