Plant Physiology Preview
Published on July 30, 2004; 10.1104/pp.104.039537
Received January 21, 2004
Returned for revision March 1, 2004
Accepted March 21, 2004
13C Labeling Reveals Chloroplastic and Extrachloroplastic Pools of Dimethylallyl Pyrophosphate and Their Contribution to Isoprene Formation
Francesco Loreto *, Paola Pinelli , Enzo Brancaleoni , and Paolo Ciccioli
Istituto di Biologia Agroambientale e Forestale (F.L., P.P.) and Istituto di Metodologie Chimiche (E.B., P.C.), Consiglio Nazionale delle Ricerche, 00016 Monterotondo Scalo, Rome, Italy
* Corresponding author; email: francesco.loreto{at}ibaf.cnr.it.
Isoprene emitted from plants is made in chloroplasts from dimethylallyl pyrophosphate (DMAPP). Leaves of Populus nigra and Phragmites australis exposed to 13CO2 for 15 min emitted isoprene that was about 90% 13C, but DMAPP isolated from those leaves was only 28% and 36% 13C, respectively. The labeled DMAPP is likely to represent chloroplastic DMAPP contributing to isoprene emission. A substantial 13C labeling was also found in both emission and DMAPP pool of low-emitting, young leaves of Phragmites. This confirms that low emission of young leaves is not caused by absence of chloroplastic DMAPP but rather by enzyme characteristics. A very low 13C labeling was found in the DMAPP pool and in the residual isoprene emission of leaves previously fed with fosmidomycin to inhibit isoprene formation. This shows that fosmidomycin is a very effective inhibitor of the chloroplastic biosynthetic pathway of isoprene synthesis, that the residual isoprene is formed from extra-chloroplastic sources, and that chloroplastic and extrachloroplastic pathways are not cross-linked, at least following inhibition of the chloroplastic pathway. Refixation of unlabeled respiratory CO2 in the light may explain incomplete labeling of isoprene emission, as we found a good association between these two parameters.
This article has been cited by other articles:
|
|
|
|
 
B. Rasulov, L. Copolovici, A. Laisk, and U. Niinemets
Postillumination Isoprene Emission: In Vivo Measurements of Dimethylallyldiphosphate Pool Size and Isoprene Synthase Kinetics in Aspen Leaves
Plant Physiology,
March 1, 2009;
149(3):
1609 - 1618.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. D. Sharkey, A. E. Wiberley, and A. R. Donohue
Isoprene Emission from Plants: Why and How
Ann. Bot.,
January 1, 2008;
101(1):
5 - 18.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Nogues, F. Brilli, and F. Loreto
Dimethylallyl Diphosphate and Geranyl Diphosphate Pools of Plant Species Characterized by Different Isoprenoid Emissions
Plant Physiology,
June 1, 2006;
141(2):
721 - 730.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Mayrhofer, M. Teuber, I. Zimmer, S. Louis, R. J. Fischbach, and J.-P. Schnitzler
Diurnal and Seasonal Variation of Isoprene Biosynthesis-Related Genes in Grey Poplar Leaves
Plant Physiology,
September 1, 2005;
139(1):
474 - 484.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Dudareva, E. Pichersky, and J. Gershenzon
Biochemistry of Plant Volatiles
Plant Physiology,
August 1, 2004;
135(4):
1893 - 1902.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Wolfertz, T. D. Sharkey, W. Boland, and F. Kuhnemann
Rapid Regulation of the Methylerythritol 4-Phosphate Pathway during Isoprene Synthesis
Plant Physiology,
August 1, 2004;
135(4):
1939 - 1945.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
