Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on July 9, 2004; 10.1104/pp.104.041699

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
135/3/1565    most recent
pp.104.041699v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (39)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Imai, A.
Right arrow Articles by Takahashi, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Imai, A.
Right arrow Articles by Takahashi, T.
Agricola
Right arrow Articles by Imai, A.
Right arrow Articles by Takahashi, T.

Received February 26, 2004
Returned for revision April 26, 2004
Accepted April 27, 2004

Spermidine Synthase Genes Are Essential for Survival of Arabidopsis

Akihiro Imai , Takashi Matsuyama , Yoshie Hanzawa , Takashi Akiyama , Masanori Tamaoki , Hikaru Saji , Yumiko Shirano , Tomohiko Kato , Hiroaki Hayashi , Daisuke Shibata , Satoshi Tabata , Yoshibumi Komeda , and Taku Takahashi *

Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan
Environmental Biology Division and Biodiversity Conservation Research Project, National Institute for Environmental Studies, Tsukuba, Ibaraki 305-0053, Japan
Department of Low-Temperature Sciences, National Agricultural Research Center for Hokkaido Region, Sapporo 062-8555, Japan
Mitsui Plant Biotechnology Research Institute (disbanded in March 1999), Tsukuba, Ibaraki 305-0047, Japan
Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan
Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
Mitsui Plant Biotechnology Research Institute (disbanded in March 1999), Tsukuba, Ibaraki 305-0047, Japan; Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan;

* Corresponding author; email: perfect{at}cc.okayama-u.ac.jp.

The cellular polyamines putrescine, spermidine, and spermine are ubiquitous in nature and have been implicated in a wide range of growth and developmental processes. There is little information, however, on mutant plants or animals defective in the synthesis of polyamines. The Arabidopsis genome has two genes encoding spermidine synthase, SPDS1 and SPDS2. In this paper, we describe T-DNA insertion mutants of both of these genes. While each mutant allele shows normal growth, spds1-1 spds2-1 double-mutant seeds are abnormally shrunken and they have embryos that are arrested morphologically at the heart-torpedo transition stage. These seeds contain significantly reduced levels of spermidine and high levels of its precursor, putrescine. The embryo lethal phenotype of spds1-1 spds2-1 is complemented by the wild-type SPDS1 gene. In addition, we observed a nearly identical seed phenotype among an F2 seed population from the cross between the spds2-1 allele and SPDS1 RNA interference transgenic lines. These data provide the first genetic evidence indicating a critical role of the spermidine synthase in plant embryo development.




This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
T. Takahashi and J.-I. Kakehi
Polyamines: ubiquitous polycations with unique roles in growth and stress responses
Ann. Bot., October 13, 2009; (2009) mcp259v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Guo, M. Baum, S. Grando, S. Ceccarelli, G. Bai, R. Li, M. von Korff, R. K. Varshney, A. Graner, and J. Valkoun
Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage
J. Exp. Bot., August 1, 2009; 60(12): 3531 - 3544.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Luo, C. Fuell, A. Parr, L. Hill, P. Bailey, K. Elliott, S. A. Fairhurst, C. Martin, and A. J. Michael
A Novel Polyamine Acyltransferase Responsible for the Accumulation of Spermidine Conjugates in Arabidopsis Seed
PLANT CELL, January 1, 2009; 21(1): 318 - 333.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. H.M. Schippers, A. Nunes-Nesi, R. Apetrei, J. Hille, A. R. Fernie, and P. P. Dijkwel
The Arabidopsis onset of leaf death5 Mutation of Quinolinate Synthase Affects Nicotinamide Adenine Dinucleotide Biosynthesis and Causes Early Ageing
PLANT CELL, October 1, 2008; 20(10): 2909 - 2925.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
E. G. Minguet, F. Vera-Sirera, A. Marina, J. Carbonell, and M. A. Blazquez
Evolutionary Diversification in Polyamine Biosynthesis
Mol. Biol. Evol., October 1, 2008; 25(10): 2119 - 2128.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J.-i. Kakehi, Y. Kuwashiro, M. Niitsu, and T. Takahashi
Thermospermine is Required for Stem Elongation in Arabidopsis thaliana
Plant Cell Physiol., September 1, 2008; 49(9): 1342 - 1349.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Kamada-Nobusada, M. Hayashi, M. Fukazawa, H. Sakakibara, and M. Nishimura
A Putative Peroxisomal Polyamine Oxidase, AtPAO4, is Involved in Polyamine Catabolism in Arabidopsis thaliana
Plant Cell Physiol., September 1, 2008; 49(9): 1272 - 1282.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
L. Muniz, E. G. Minguet, S. K. Singh, E. Pesquet, F. Vera-Sirera, C. L. Moreau-Courtois, J. Carbonell, M. A. Blazquez, and H. Tuominen
ACAULIS5 controls Arabidopsis xylem specification through the prevention of premature cell death
Development, August 1, 2008; 135(15): 2573 - 2582.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Vigeolas, C. Chinoy, E. Zuther, B. Blessington, P. Geigenberger, and C. Domoney
Combined Metabolomic and Genetic Approaches Reveal a Link between the Polyamine Pathway and Albumin 2 in Developing Pea Seeds
Plant Physiology, January 1, 2008; 146(1): 74 - 82.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Vuosku, A. Jokela, E. Laara, M. Saaskilahti, R. Muilu, S. Sutela, T. Altabella, T. Sarjala, and H. Haggman
Consistency of Polyamine Profiles and Expression of Arginine Decarboxylase in Mitosis during Zygotic Embryogenesis of Scots Pine
Plant Physiology, November 1, 2006; 142(3): 1027 - 1038.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Imai, Y. Hanzawa, M. Komura, K. T. Yamamoto, Y. Komeda, and T. Takahashi
The dwarf phenotype of the Arabidopsis acl5 mutant is suppressed by a mutation in an upstream ORF of a bHLH gene
Development, September 15, 2006; 133(18): 3575 - 3585.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Tavladoraki, M. N. Rossi, G. Saccuti, M. A. Perez-Amador, F. Polticelli, R. Angelini, and R. Federico
Heterologous Expression and Biochemical Characterization of a Polyamine Oxidase from Arabidopsis Involved in Polyamine Back Conversion
Plant Physiology, August 1, 2006; 141(4): 1519 - 1532.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. Van de Velde, J. C. P. Guerra, A. D. Keyser, R. De Rycke, S. Rombauts, N. Maunoury, P. Mergaert, E. Kondorosi, M. Holsters, and S. Goormachtig
Aging in Legume Symbiosis. A Molecular View on Nodule Senescence in Medicago truncatula
Plant Physiology, June 1, 2006; 141(2): 711 - 720.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
Y. Ikeguchi, M. C. Bewley, and A. E. Pegg
Aminopropyltransferases: Function, Structure and Genetics
J. Biochem., January 1, 2006; 139(1): 1 - 9.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. A. Paschalidis and K. A. Roubelakis-Angelakis
Sites and Regulation of Polyamine Catabolism in the Tobacco Plant. Correlations with Cell Division/Expansion, Cell Cycle Progression, and Vascular Development
Plant Physiology, August 1, 2005; 138(4): 2174 - 2184.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2004 by the American Society of Plant Biologists