Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on August 6, 2004; 10.1104/pp.104.043398

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
135/4/2230    most recent
pp.104.043398v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Campanella, J. J.
Right arrow Articles by Ludwig-Müller, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Campanella, J. J.
Right arrow Articles by Ludwig-Müller, J.
Agricola
Right arrow Articles by Campanella, J. J.
Right arrow Articles by Ludwig-Müller, J.

Received March 23, 2004
Returned for revision June 4, 2004
Accepted June 7, 2004

A Novel Auxin Conjugate Hydrolase from Wheat with Substrate Specificity for Longer Side-Chain Auxin Amide Conjugates

James J. Campanella *, Adebanke F. Olajide , Volker Magnus , and Jutta Ludwig-Müller

Department of Biology and Molecular Biology, Montclair State University, Montclair, New Jersey 07043 (J.J.C., A.F.O.); Rudjer Boskovic Institute, 10002 Zagreb, Croatia (V.M.); and Institut für Botanik, Technische Universität Dresden, 01062 Dresden, Germany (J.L.-M.)

* Corresponding author; email: james.campanella{at}montclair.edu.

This study investigates how the ILR1-like indole acetic acid (IAA) amidohydrolase family of genes has functionally evolved in the monocotyledonous species wheat (Triticum aestivum). An ortholog for the Arabidopsis IAR3 auxin amidohydrolase gene has been isolated from wheat (TaIAR3). The TaIAR3 protein hydrolyzes negligible levels of IAA-Ala and no other IAA amino acid conjugates tested, unlike its ortholog IAR3. Instead, TaIAR3 has low specificity for the ester conjugates IAA-Glc and IAA-myoinositol and high specificity for the conjugates of indole-3-butyric acid (IBA-Ala and IBA-Gly) and indole-3-propionic-acid (IPA-Ala) so far tested. TaIAR3 did not convert the methyl esters of the IBA conjugates with Ala and Gly. IBA and IBA conjugates were detected in wheat seedlings by gas chromatography-mass spectrometry, where the conjugate of IBA with Ala may serve as a natural substrate for this enzyme. Endogenous IPA and IPA conjugates were not detected in the seedlings. Additionally, crude protein extracts of wheat seedlings possess auxin amidohydrolase activity. Temporal expression studies of TaIAR3 indicate that the transcript is initially expressed at day 1 after germination. Expression decreases through days 2, 5, 10, 15, and 20. Spatial expression studies found similar levels of expression throughout all wheat tissues examined.




This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
B. Savic, S. Tomic, V. Magnus, K. Gruden, K. Barle, R. Grenkovic, J. Ludwig-Muller, and B. Salopek-Sondi
Auxin Amidohydrolases from Brassica rapa Cleave the Alanine Conjugate of Indolepropionic Acid as a Preferable Substrate: A Biochemical and Modeling Approach
Plant Cell Physiol., September 1, 2009; 50(9): 1587 - 1599.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Fait, R. Angelovici, H. Less, I. Ohad, E. Urbanczyk-Wochniak, A. R. Fernie, and G. Galili
Arabidopsis Seed Development and Germination Is Associated with Temporally Distinct Metabolic Switches
Plant Physiology, November 1, 2006; 142(3): 839 - 854.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Wingler, S. Purdy, J. A. MacLean, and N. Pourtau
The role of sugars in integrating environmental signals during the regulation of leaf senescence
J. Exp. Bot., January 1, 2006; 57(2): 391 - 399.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
A. W. WOODWARD and B. BARTEL
Auxin: Regulation, Action, and Interaction
Ann. Bot., April 1, 2005; 95(5): 707 - 735.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2004 by the American Society of Plant Biologists