Plant Physiology Preview
Published on August 27, 2004; 10.1104/pp.104.044610
Received April 14, 2004
Returned for revision May 30, 2004
Accepted June 4, 2004
Genomic Analysis of the Nitrate Response Using a Nitrate Reductase-Null Mutant of Arabidopsis
Rongchen Wang , Rudolf Tischner , Rodrigo A. Gutiérrez , Maren Hoffman , Xiujuan Xing , Mingsheng Chen , Gloria Coruzzi , and Nigel M. Crawford *
Section of Cell and Developmental Biology, Division of Biological Sciences, University of California at San Diego, La Jolla, California 92093-0116
Albrecht von Haller Institut für Pflanzenwissenschaften, University of Gottingen, 37073 Gottingen, Germany
Department of Biology, New York University, New York, New York 10003
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
* Corresponding author; email: ncrawford{at}ucsd.edu.
A nitrate reductase (NR)-null mutant of Arabidopsis was constructed that had a deletion of the major NR gene NIA2 and an insertion in the NIA1 NR gene. This mutant had no detectable NR activity and could not use nitrate as the sole nitrogen source. Starch mobilization was not induced by nitrate in this mutant but was induced by ammonium, indicating that nitrate was not the signal for this process. Microarray analysis of gene expression revealed that 595 genes responded to nitrate (5 mM nitrate for 2 h) in both wild-type and mutant plants. This group of genes was overrepresented most significantly in the functional categories of energy, metabolism, and glycolysis and gluconeogenesis. Because the nitrate response of these genes was NR independent, nitrate and not a downstream metabolite served as the signal. The microarray analysis also revealed that shoots can be as responsive to nitrate as roots, yet there was substantial organ specificity to the nitrate response.
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