Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on October 1, 2004; 10.1104/pp.104.044784

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
136/2/3114    most recent
pp.104.044784v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (78)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liszkay, A.
Right arrow Articles by Schopfer, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liszkay, A.
Right arrow Articles by Schopfer, P.
Agricola
Right arrow Articles by Liszkay, A.
Right arrow Articles by Schopfer, P.

Received April 20, 2004
Returned for revision June 23, 2004
Accepted June 28, 2004

Production of Reactive Oxygen Intermediates (O2·-, H2O2, and ·OH) by Maize Roots and Their Role in Wall Loosening and Elongation Growth

Anja Liszkay , Esther van der Zalm , and Peter Schopfer *

Institut für Biologie II der Universität, D-79104 Freiburg, Germany

* Corresponding author; email: peter.schopfer{at}biologie.uni-freiburg.de.

Cell extension in the growing zone of plant roots typically takes place with a maximum local growth rate of 50% length increase per hour. The biochemical mechanism of this dramatic growth process is still poorly understood. Here we test the hypothesis that the wall-loosening reaction controlling root elongation is effected by the production of reactive oxygen intermediates, initiated by a NAD(P)H oxidase-catalyzed formation of superoxide radicals (O2·-) at the plasma membrane and culminating in the generation of polysaccharide-cleaving hydroxyl radicals (·OH) by cell wall peroxidase. The following results were obtained using primary roots of maize (Zea mays) seedlings as experimental material. (1) Production of O2·-, H2O2, and ·OH can be demonstrated in the growing zone using specific histochemical assays and electron paramagnetic resonance spectroscopy. (2) Auxin-induced inhibition of growth is accompanied by a reduction of O2·- production. (3) Experimental generation of ·OH in the cell walls with the Fenton reaction causes wall loosening (cell wall creep), specifically in the growing zone. Alternatively, wall loosening can be induced by ·OH produced by endogenous cell wall peroxidase in the presence of NADH and H2O2. (4) Inhibition of endogenous ·OH formation by O2·- or ·OH scavengers, or inhibitors of NAD(P)H oxidase or peroxidase activity, suppress elongation growth. These results show that juvenile root cells transiently express the ability to generate ·OH, and to respond to ·OH by wall loosening, in passing through the growing zone. Moreover, inhibitor studies indicate that ·OH formation is essential for normal root growth.




This article has been cited by other articles:


Home page
 J Exp BotHome page
A. A. Rodriguez, S. J. Maiale, A. B. Menendez, and O. A. Ruiz
Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress
J. Exp. Bot., November 1, 2009; 60(15): 4249 - 4262.
[Abstract] [Full Text] [PDF]

Home page
 Tree PhysiolHome page
M. Alvarez, D. Huygens, C. Fernandez, Y. Gacitua, E. Olivares, I. Saavedra, M. Alberdi, and E. Valenzuela
Effect of ectomycorrhizal colonization and drought on reactive oxygen species metabolism of Nothofagus dombeyi roots
Tree Physiol, August 1, 2009; 29(8): 1047 - 1057.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Muller, A. Linkies, R. A.M. Vreeburg, S. C. Fry, A. Krieger-Liszkay, and G. Leubner-Metzger
In Vivo Cell Wall Loosening by Hydroxyl Radicals during Cress Seed Germination and Elongation Growth
Plant Physiology, August 1, 2009; 150(4): 1855 - 1865.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Oracz, H. El-Maarouf-Bouteau, I. Kranner, R. Bogatek, F. Corbineau, and C. Bailly
The Mechanisms Involved in Seed Dormancy Alleviation by Hydrogen Cyanide Unravel the Role of Reactive Oxygen Species as Key Factors of Cellular Signaling during Germination
Plant Physiology, May 1, 2009; 150(1): 494 - 505.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Cosio and C. Dunand
Specific functions of individual class III peroxidase genes
J. Exp. Bot., February 1, 2009; 60(2): 391 - 408.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. Marjamaa, E. M. Kukkola, and K. V. Fagerstedt
The role of xylem class III peroxidases in lignification
J. Exp. Bot., February 1, 2009; 60(2): 367 - 376.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
B. Kukavica, M. Mojovic, Z. Vuccinic, V. Maksimovic, U. Takahama, and S. V. Jovanovic
Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production
Plant Cell Physiol., February 1, 2009; 50(2): 304 - 317.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Laohavisit, J. C. Mortimer, V. Demidchik, K. M. Coxon, M. A. Stancombe, N. Macpherson, C. Brownlee, A. Hofmann, A. A.R. Webb, H. Miedema, et al.
Zea mays Annexins Modulate Cytosolic Free Ca2+ and Generate a Ca2+-Permeable Conductance
PLANT CELL, February 1, 2009; 21(2): 479 - 493.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. Gutjahr, M. Banba, V. Croset, K. An, A. Miyao, G. An, H. Hirochika, H. Imaizumi-Anraku, and U. Paszkowski
Arbuscular Mycorrhiza-Specific Signaling in Rice Transcends the Common Symbiosis Signaling Pathway
PLANT CELL, November 1, 2008; 20(11): 2989 - 3005.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
D. Bustos, R. Lascano, A. L. Villasuso, E. Machado, M. E. Senn, A. Cordoba, and E. Taleisnik
Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2*- Levels
Ann. Bot., October 1, 2008; 102(4): 551 - 559.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Zhu, S. Alvarez, E. L. Marsh, M. E. LeNoble, I.-J. Cho, M. Sivaguru, S. Chen, H. T. Nguyen, Y. Wu, D. P. Schachtman, et al.
Cell Wall Proteome in the Maize Primary Root Elongation Zone. II. Region-Specific Changes in Water Soluble and Lightly Ionically Bound Proteins under Water Deficit
Plant Physiology, December 1, 2007; 145(4): 1533 - 1548.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
A. H. L. A. N. Gunawardena, J. S. Greenwood, and N. G. Dengler
Cell wall degradation and modification during programmed cell death in lace plant, Aponogeton madagascariensis (Aponogetonaceae)
Am. J. Botany, July 1, 2007; 94(7): 1116 - 1128.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. P. Bienert, A. L. B. Moller, K. A. Kristiansen, A. Schulz, I. M. Moller, J. K. Schjoerring, and T. P. Jahn
Specific Aquaporins Facilitate the Diffusion of Hydrogen Peroxide across Membranes
J. Biol. Chem., January 12, 2007; 282(2): 1183 - 1192.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Trujillo, L. Altschmied, P. Schweizer, K.-H. Kogel, and R. Huckelhoven
Respiratory Burst Oxidase Homologue A of barley contributes to penetration by the powdery mildew fungus Blumeria graminis f. sp. hordei
J. Exp. Bot., November 1, 2006; 57(14): 3781 - 3791.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
N. FEDOROFF
Redox Regulatory Mechanisms in Cellular Stress Responses
Ann. Bot., August 1, 2006; 98(2): 289 - 300.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Cona, G. Rea, M. Botta, F. Corelli, R. Federico, and R. Angelini
Flavin-containing polyamine oxidase is a hydrogen peroxide source in the oxidative response to the protein phosphatase inhibitor cantharidin in Zea mays L.
J. Exp. Bot., July 1, 2006; 57(10): 2277 - 2289.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Gapper and L. Dolan
Control of Plant Development by Reactive Oxygen Species
Plant Physiology, June 1, 2006; 141(2): 341 - 345.
[Full Text] [PDF]

Home page
 J Exp BotHome page
A. Karkonen and S. C Fry
Effect of ascorbate and its oxidation products on H2O2 production in cell-suspension cultures of Picea abies and in the absence of cells
J. Exp. Bot., May 1, 2006; 57(8): 1633 - 1644.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. J Carol and L. Dolan
The role of reactive oxygen species in cell growth: lessons from root hairs
J. Exp. Bot., May 1, 2006; 57(8): 1829 - 1834.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Karlsson, M. Melzer, I. Prokhorenko, T. Johansson, and G. Wingsle
Hydrogen peroxide and expression of hipI-superoxide dismutase are associated with the development of secondary cell walls in Zinnia elegans
J. Exp. Bot., August 1, 2005; 56(418): 2085 - 2093.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Guimil, H.-S. Chang, T. Zhu, A. Sesma, A. Osbourn, C. Roux, V. Ioannidis, E. J. Oakeley, M. Docquier, P. Descombes, et al.
Comparative transcriptomics of rice reveals an ancient pattern of response to microbial colonization
PNAS, May 31, 2005; 102(22): 8066 - 8070.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2004 by the American Society of Plant Biologists