Plant Physiology Preview
Published on July 30, 2004; 10.1104/pp.104.045542
Received April 29, 2004
Returned for revision June 1, 2004
Accepted June 2, 2004
Characterization of the Arabidopsis Lysine-Rich Arabinogalactan-Protein AtAGP17 Mutant (rat1) That Results in a Decreased Efficiency of Agrobacterium Transformation
Yolanda Maria Gaspar , Jaesung Nam , Carolyn Jane Schultz , Lan-Ying Lee , Paul R. Gilson , Stanton B. Gelvin , and Antony Bacic *
Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, Victoria 3010, Australia (Y.M.G., P.R.G., A.B.); Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392 (J.N., L.-Y.L., S.B.G.); and School of Agriculture and Wine, Waite Agricultural Research Institute, The University of Adelaide, South Australia 5064, Australia (C.J.S.)
* Corresponding author; email: abacic{at}unimelb.edu.au.
Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans widely distributed in plants. The Arabidopsis rat1 mutant, previously characterized as resistant to Agrobacterium tumefaciens root transformation, is due to a mutation in the gene for the Lys-rich AGP, AtAGP17. We show that the phenotype of rat1 correlates with down-regulation of AGP17 in the root as a result of a T-DNA insertion into the promoter of AGP17. Complementation of rat1 plants by a floral dip method with either the wild-type AGP17 gene or cDNA can restore the plant to a wild-type phenotype in several independent transformants. Based on changes in PR1 gene expression and a decrease in free salicylic acid levels upon Agrobacterium infection, we suggest mechanisms by which AGP17 allows Agrobacterium rapidly to reduce the systemic acquired resistance response during the infection process.
This article has been cited by other articles:
|
|
|
|
 
S. B. Gelvin
Agrobacterium in the Genomics Age
Plant Physiology,
August 1, 2009;
150(4):
1665 - 1676.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Zheng, X.-W. He, Y.-H. Ying, J.-F. Lu, S. B. Gelvin, and H.-X. Shou
Expression of the Arabidopsis thaliana Histone Gene AtHTA1 Enhances Rice Transformation Efficiency
Mol Plant,
July 1, 2009;
2(4):
832 - 837.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Coimbra, M. Costa, B. Jones, M. A. Mendes, and L. G. Pereira
Pollen grain development is compromised in Arabidopsis agp6 agp11 null mutants
J. Exp. Bot.,
July 1, 2009;
60(11):
3133 - 3142.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Estevez, P. V. Fernandez, L. Kasulin, P. Dupree, and M. Ciancia
Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile
Glycobiology,
March 1, 2009;
19(3):
212 - 228.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Anand, S. R. Uppalapati, C.-M. Ryu, S. N. Allen, L. Kang, Y. Tang, and K. S. Mysore
Salicylic Acid and Systemic Acquired Resistance Play a Role in Attenuating Crown Gall Disease Caused by Agrobacterium tumefaciens
Plant Physiology,
February 1, 2008;
146(2):
703 - 715.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L.-Y. Lee, M. E. Kononov, B. Bassuner, B. R. Frame, K. Wang, and S. B. Gelvin
Novel Plant Transformation Vectors Containing the Superpromoter
Plant Physiology,
December 1, 2007;
145(4):
1294 - 1300.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-C. Yuan, M. P. Edlind, P. Liu, P. Saenkham, L. M. Banta, A. A. Wise, E. Ronzone, A. N. Binns, K. Kerr, and E. W. Nester
The plant signal salicylic acid shuts down expression of the vir regulon and activates quormone-quenching genes in Agrobacterium
PNAS,
July 10, 2007;
104(28):
11790 - 11795.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. S. Sardar, J. Yang, and A. M. Showalter
Molecular Interactions of Arabinogalactan Proteins with Cortical Microtubules and F-Actin in Bright Yellow-2 Tobacco Cultured Cells
Plant Physiology,
December 1, 2006;
142(4):
1469 - 1479.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Estevez, M. J. Kieliszewski, N. Khitrov, and C. Somerville
Characterization of Synthetic Hydroxyproline-Rich Proteoglycans with Arabinogalactan Protein and Extensin Motifs in Arabidopsis
Plant Physiology,
October 1, 2006;
142(2):
458 - 470.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. J.D. Lee, Y. Sakata, S.-L. Mau, F. Pettolino, A. Bacic, R. S. Quatrano, C. D. Knight, and J. P. Knox
Arabinogalactan Proteins Are Required for Apical Cell Extension in the Moss Physcomitrella patens
PLANT CELL,
November 1, 2005;
17(11):
3051 - 3065.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
