Plant Physiology Preview
Published on November 5, 2004; 10.1104/pp.104.045625
Received April 30, 2004
Returned for revision June 3, 2004
Accepted June 7, 2004
Root-to-Shoot Transport of Sulfate in Arabidopsis. Evidence for the Role of SULTR3;5 as a Component of Low-Affinity Sulfate Transport System in the Root Vasculature
Tatsuhiko Kataoka , Naomi Hayashi , Tomoyuki Yamaya , and Hideki Takahashi *
RIKEN Plant Science Center, Tsurumi-ku, Yokohama 230-0045, Japan
RIKEN Plant Science Center, Tsurumi-ku, Yokohama 230-0045, Japan; Tohoku University, Graduate School of Agricultural Science, Aoba-ku, Sendai 981-8555, Japan
* Corresponding author; email: hideki{at}postman.riken.go.jp.
Xylem transport of sulfate regulates distribution of sulfur in vascular plants. Here, we describe SULTR3;5 as an essential component of the sulfate transport system that facilitates the root-to-shoot transport of sulfate in the vasculature. In Arabidopsis (Arabidopsis thaliana), SULTR3;5 was colocalized with the SULTR2;1 low-affinity sulfate transporter in xylem parenchyma and pericycle cells in roots. In a yeast (Saccharomyces cerevisiae) expression system, sulfate uptake was hardly detectable with SULTR3;5 expression alone; however, cells coexpressing both SULTR3;5 and SULTR2;1 showed substantial uptake activity that was considerably higher than with SULTR2;1 expression alone. The Vmax value of sulfate uptake activity with SULTR3;5-SULTR2;1 coexpression was approximately 3 times higher than with SULTR2;1 alone. In Arabidopsis, the root-to-shoot transport of sulfate was restricted in the sultr3;5 mutants, under conditions of high SULTR2;1 expression in the roots after sulfur limitation. These results suggested that SULTR3;5 is constitutively expressed in the root vasculature, but its function to reinforce the capacity of the SULTR2;1 low-affinity transporter is only essential when SULTR2;1 mRNA is induced by sulfur limitation. Consequently, coexpression of SULTR3;5 and SULTR2;1 provides maximum capacity of sulfate transport activity, which facilitates retrieval of apoplastic sulfate to the xylem parenchyma cells in the vasculature of Arabidopsis roots and may contribute to the root-to-shoot transport of sulfate.
This article has been cited by other articles:
|
|
|
|
 
S.-H. Lin, H.-F. Kuo, G. Canivenc, C.-S. Lin, M. Lepetit, P.-K. Hsu, P. Tillard, H.-L. Lin, Y.-Y. Wang, C.-B. Tsai, et al.
Mutation of the Arabidopsis NRT1.5 Nitrate Transporter Causes Defective Root-to-Shoot Nitrate Transport
PLANT CELL,
September 1, 2008;
20(9):
2514 - 2528.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Endo, Y. Sawada, H. Takahashi, M. Okamoto, K. Ikegami, H. Koiwai, M. Seo, T. Toyomasu, W. Mitsuhashi, K. Shinozaki, et al.
Drought Induction of Arabidopsis 9-cis-Epoxycarotenoid Dioxygenase Occurs in Vascular Parenchyma Cells
Plant Physiology,
August 1, 2008;
147(4):
1984 - 1993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Rouached, M. Wirtz, R. Alary, R. Hell, A. B. Arpat, J.-C. Davidian, P. Fourcroy, and P. Berthomieu
Differential Regulation of the Expression of Two High-Affinity Sulfate Transporters, SULTR1.1 and SULTR1.2, in Arabidopsis
Plant Physiology,
June 1, 2008;
147(2):
897 - 911.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Yoshimoto, E. Inoue, A. Watanabe-Takahashi, K. Saito, and H. Takahashi
Posttranscriptional Regulation of High-Affinity Sulfate Transporters in Arabidopsis by Sulfur Nutrition
Plant Physiology,
October 1, 2007;
145(2):
378 - 388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. El Kassis, N. Cathala, H. Rouached, P. Fourcroy, P. Berthomieu, N. Terry, and J.-C. Davidian
Characterization of a Selenate-Resistant Arabidopsis Mutant. Root Growth as a Potential Target for Selenate Toxicity
Plant Physiology,
March 1, 2007;
143(3):
1231 - 1241.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Maruyama-Nakashita, Y. Nakamura, T. Tohge, K. Saito, and H. Takahashi
Arabidopsis SLIM1 Is a Central Transcriptional Regulator of Plant Sulfur Response and Metabolism
PLANT CELL,
November 1, 2006;
18(11):
3235 - 3251.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. KOPRIVA
Regulation of Sulfate Assimilation in Arabidopsis and Beyond
Ann. Bot.,
April 1, 2006;
97(4):
479 - 495.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Krusell, K. Krause, T. Ott, G. Desbrosses, U. Kramer, S. Sato, Y. Nakamura, S. Tabata, E. K. James, N. Sandal, et al.
The Sulfate Transporter SST1 Is Crucial for Symbiotic Nitrogen Fixation in Lotus japonicus Root Nodules
PLANT CELL,
May 1, 2005;
17(5):
1625 - 1636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Hopkins, S. Parmar, A. Blaszczyk, H. Hesse, R. Hoefgen, and M. J. Hawkesford
O-Acetylserine and the Regulation of Expression of Genes Encoding Components for Sulfate Uptake and Assimilation in Potato
Plant Physiology,
May 1, 2005;
138(1):
433 - 440.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Hirose, N. Makita, T. Yamaya, and H. Sakakibara
Functional Characterization and Expression Analysis of a Gene, OsENT2, Encoding an Equilibrative Nucleoside Transporter in Rice Suggest a Function in Cytokinin Transport
Plant Physiology,
May 1, 2005;
138(1):
196 - 206.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
