Plant Physiology Preview
Published on October 15, 2004; 10.1104/pp.104.046961
Received May 25, 2004
Returned for revision August 8, 2004
Accepted August 9, 2004
Ca2+ Dynamics in a Pollen Grain and Papilla Cell during Pollination of Arabidopsis
Megumi Iwano *, Hiroshi Shiba , Teruhiko Miwa , Fang-Sik Che , Seiji Takayama , Takeharu Nagai , Atsushi Miyawaki , and Akira Isogai
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0101, Japan
The Institute of Physical and Chemical Research, Wako, Saitama 351-0198, Japan
* Corresponding author; email: m-iwano{at}bs.naist.jp.
Ca2+ dynamics in the growing pollen tube have been well documented in vitro using germination assays and Ca2+ imaging techniques. However, very few in vivo studies of Ca2+ in the pollen grain and papilla cell during pollination have been performed. We expressed yellow cameleon, a Ca2+ indicator based on green fluorescent protein, in the pollen grains and papilla cells of Arabidopsis (Arabidopsis thaliana) and monitored Ca2+ dynamics during pollination. In the pollen grain, [Ca2+]cyt increased at the potential germination site soon after hydration and remained augmented until germination. As in previous in vitro germination studies, [Ca2+]cyt oscillations were observed in the tip region of the growing pollen tube, but the oscillation frequency was faster and [Ca2+]cyt was higher than had been observed in vitro. In the pollinated papilla cell, remarkable increases in [Ca2+]cyt occurred three times in succession, just under the site of pollen-grain attachment. [Ca2+]cyt increased first soon after pollen hydration, with a second increase occurring after pollen protrusion. The third and most remarkable [Ca2+]cyt increase took place when the pollen tube penetrated into the papilla cell wall.
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