Plant Physiology Preview
Published on December 3, 2004; 10.1104/pp.104.049361
Received July 8, 2004
Returned for revision October 8, 2004
Accepted October 12, 2004
The Role of the Cell Cycle Machinery in Resumption of Postembryonic Development
Rosa Maria Barrôco , Kris Van Poucke , Jan H.W. Bergervoet , Lieven De Veylder , Steven P.C. Groot , Dirk Inzé *, and Gilbert Engler
Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, B-9052 Gent, Belgium
Plant Research International, NL-6700 AA Wageningen, The Netherlands
Laboratoire Associé de l'Institut National de la Recherche Agronomique (France), Ghent University, B-9000 Gent, Belgium
* Corresponding author; email: dirk.inze{at}psb.ugent.be.
Cell cycle activity is required for plant growth and development, but its involvement in the early events that initiate seedling development remains to be clarified. We performed experiments aimed at understanding when cell cycle progression is activated during seed germination, and what its contribution is for proper seedling establishment. To this end, the spatial and temporal expression profiles of a large set of cell cycle control genes in germinating seeds of Arabidopsis (Arabidopsis thaliana) and white cabbage (Brassica oleracea) were analyzed. The in vivo behavior of the microtubular cytoskeleton was monitored during Arabidopsis seed germination. Flow cytometry of Arabidopsis germinating seeds indicated that DNA replication was mainly initiated at the onset of root protrusion, when germination reached its end. Expression analysis of cell cycle genes with mRNA in situ localization,  -glucuronidase assays, and semiquantitative reverse transcription-polymerase chain reaction showed that transcription of most cell cycle genes was detected only after completion of germination. In vivo green fluorescent protein analysis of the microtubule cytoskeleton demonstrated that mitosis-specific microtubule arrays occurred only when the radicle had started to protrude, although the assembly of the microtubular cytoskeleton was promptly activated once germination was initiated. Thus, seed germination involves the synthesis and/or activation of a reduced number of core cell cycle proteins, which only trigger DNA replication, but is not sufficient to drive cells into mitosis. Mitotic divisions are observed only after the radicle has protruded and presumably rely on the de novo production of other cell cycle regulators.
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