Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on October 29, 2004; 10.1104/pp.104.049718

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
136/3/3504    most recent
pp.104.049718v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (48)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Simkin, A. J.
Right arrow Articles by Klee, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Simkin, A. J.
Right arrow Articles by Klee, H. J.
Agricola
Right arrow Articles by Simkin, A. J.
Right arrow Articles by Klee, H. J.

Received July 13, 2004
Returned for revision August 20, 2004
Accepted August 20, 2004

Circadian Regulation of the PhCCD1 Carotenoid Cleavage Dioxygenase Controls Emission of {beta}-Ionone, a Fragrance Volatile of Petunia Flowers

Andrew J. Simkin , Beverly A. Underwood , Michele Auldridge , Holly M. Loucas , Kenichi Shibuya , Eric Schmelz , David G. Clark , and Harry J. Klee *

Horticultural Sciences, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, Florida 32611-0690
Environmental Horticulture, University of Florida, Gainesville, Florida 32611-0670
United States Department of Agriculture, Agricultural Research Service, Center for Medical Agricultural and Veterinary Entomology, Gainesville, Florida 32608

* Corresponding author; email: hjklee{at}ifas.ufl.edu.

Carotenoids are thought to be the precursors of terpenoid volatile compounds that contribute to flavor and aroma. One such volatile, {beta}-ionone, is important to fragrance in many flowers, including petunia (Petunia hybrida). However, little is known about the factors regulating its synthesis in vivo. The petunia genome contains a gene encoding a 9,10(9',10') carotenoid cleavage dioxygenase, PhCCD1. The PhCCD1 is 94% identical to LeCCD1A, an enzyme responsible for formation of {beta}-ionone in tomato (Lycopersicon esculentum; Simkin AJ, Schwartz SH, Auldridge M, Taylor MG, Klee HJ [2004] Plant J [in press]). Reduction of PhCCD1 transcript levels in transgenic plants led to a 58% to 76% decrease in {beta}-ionone synthesis in the corollas of selected petunia lines, indicating a significant role for this enzyme in volatile synthesis. Quantitative reverse transcription-PCR analysis revealed that PhCCD1 is highly expressed in corollas and leaves, where it constitutes approximately 0.04% and 0.02% of total RNA, respectively. PhCCD1 is light-inducible and exhibits a circadian rhythm in both leaves and flowers. {beta}-Ionone emission by flowers occurred principally during daylight hours, paralleling PhCCD1 expression in corollas. The results indicate that PhCCD1 activity and {beta}-ionone emission are likely regulated at the level of transcript.




This article has been cited by other articles:


Home page
 J Exp BotHome page
F.-C. Huang, P. Molnar, and W. Schwab
Cloning and functional characterization of carotenoid cleavage dioxygenase 4 genes
J. Exp. Bot., July 1, 2009; 60(11): 3011 - 3022.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. S. Floss, W. Schliemann, J. Schmidt, D. Strack, and M. H. Walter
RNA Interference-Mediated Repression of MtCCD1 in Mycorrhizal Roots of Medicago truncatula Causes Accumulation of C27 Apocarotenoids, Shedding Light on the Functional Role of CCD1
Plant Physiology, November 1, 2008; 148(3): 1267 - 1282.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Rubio, J. L. Rambla, M. Santaella, M. D. Gomez, D. Orzaez, A. Granell, and L. Gomez-Gomez
Cytosolic and Plastoglobule-targeted Carotenoid Dioxygenases from Crocus sativus Are Both Involved in {beta}-Ionone Release
J. Biol. Chem., September 5, 2008; 283(36): 24816 - 24825.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. T. Vogel, B.-C. Tan, D. R. McCarty, and H. J. Klee
The Carotenoid Cleavage Dioxygenase 1 Enzyme Has Broad Substrate Specificity, Cleaving Multiple Carotenoids at Two Different Bond Positions
J. Biol. Chem., April 25, 2008; 283(17): 11364 - 11373.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Spitzer, M. M. B. Zvi, M. Ovadis, E. Marhevka, O. Barkai, O. Edelbaum, I. Marton, T. Masci, M. Alon, S. Morin, et al.
Reverse Genetics of Floral Scent: Application of Tobacco Rattle Virus-Based Gene Silencing in Petunia
Plant Physiology, December 1, 2007; 145(4): 1241 - 1250.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. Prado-Cabrero, D. Scherzinger, J. Avalos, and S. Al-Babili
Retinal Biosynthesis in Fungi: Characterization of the Carotenoid Oxygenase CarX from Fusarium fujikuroi
Eukaryot. Cell, April 1, 2007; 6(4): 650 - 657.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
M. Jaquinod, F. Villiers, S. Kieffer-Jaquinod, V. Hugouvieux, C. Bruley, J. Garin, and J. Bourguignon
A Proteomics Dissection of Arabidopsis thaliana Vacuoles Isolated from Cell Culture
Mol. Cell. Proteomics, March 1, 2007; 6(3): 394 - 412.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Loivamaki, S. Louis, G. Cinege, I. Zimmer, R. J. Fischbach, and J.-P. Schnitzler
Circadian Rhythms of Isoprene Biosynthesis in Grey Poplar Leaves
Plant Physiology, January 1, 2007; 143(1): 540 - 551.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Ohmiya, S. Kishimoto, R. Aida, S. Yoshioka, and K. Sumitomo
Carotenoid Cleavage Dioxygenase (CmCCD4a) Contributes to White Color Formation in Chrysanthemum Petals
Plant Physiology, November 1, 2006; 142(3): 1193 - 1201.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Kato, H. Matsumoto, Y. Ikoma, H. Okuda, and M. Yano
The role of carotenoid cleavage dioxygenases in the regulation of carotenoid profiles during maturation in citrus fruit
J. Exp. Bot., July 1, 2006; 57(10): 2153 - 2164.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Carrari and A. R. Fernie
Metabolic regulation underlying tomato fruit development
J. Exp. Bot., June 1, 2006; 57(9): 1883 - 1897.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Schmidt, R. Kurtzer, W. Eisenreich, and W. Schwab
The Carotenase AtCCD1 from Arabidopsis thaliana Is a Dioxygenase
J. Biol. Chem., April 14, 2006; 281(15): 9845 - 9851.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Mathieu, N. Terrier, J. Procureur, F. Bigey, and Z. Gunata
A Carotenoid Cleavage Dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation
J. Exp. Bot., October 1, 2005; 56(420): 2721 - 2731.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. A. Underwood, D. M. Tieman, K. Shibuya, R. J. Dexter, H. M. Loucas, A. J. Simkin, C. A. Sims, E. A. Schmelz, H. J. Klee, and D. G. Clark
Ethylene-Regulated Floral Volatile Synthesis in Petunia Corollas
Plant Physiology, May 1, 2005; 138(1): 255 - 266.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. C. Snowden, A. J. Simkin, B. J. Janssen, K. R. Templeton, H. M. Loucas, J. L. Simons, S. Karunairetnam, A. P. Gleave, D. G. Clark, and H. J. Klee
The Decreased apical dominance1/Petunia hybrida CAROTENOID CLEAVAGE DIOXYGENASE8 Gene Affects Branch Production and Plays a Role in Leaf Senescence, Root Growth, and Flower Development
PLANT CELL, March 1, 2005; 17(3): 746 - 759.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2004 by the American Society of Plant Biologists