Plant Physiology Preview
Published on October 15, 2004; 10.1104/pp.104.050153
Received July 18, 2004
Returned for revision August 29, 2004
Accepted August 30, 2004
Unexpected Deposition Patterns of Recombinant Proteins in Post-Endoplasmic Reticulum Compartments of Wheat Endosperm
Elsa Arcalis , Sylvain Marcel , Friedrich Altmann , Daniel Kolarich , Georgia Drakakaki , Rainer Fischer , Paul Christou , and Eva Stoger *
Institute for Molecular Biotechnology (Biology VII), Rheinische-Westfälische Technische Hochschule Aachen, 52074 Aachen, Germany
Department of Chemistry, Glycobiology Division, University of Natural Resources and Applied Life Sciences, 1190 Vienna, Austria
Institute for Molecular Biotechnology (Biology VII), Rheinische-Westfälische Technische Hochschule Aachen, 52074 Aachen, Germany; Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), 57392 Schmallenberg, Germany
Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), 57392 Schmallenberg, Germany
* Corresponding author; email: eva.stoger{at}molbiotech.rwth-aachen.de.
Protein transport within cereal endosperm cells is complicated by the abundance of endoplasmic reticulum (ER)-derived and vacuolar protein bodies. For wheat storage proteins, two major transport routes run from the ER to the vacuole, one bypassing and one passing through the Golgi. Proteins traveling along each route converge at the vacuole and form aggregates. To determine the impact of this trafficking system on the fate of recombinant proteins expressed in wheat endosperm, we used confocal and electron microscopy to investigate the fate of three recombinant proteins containing different targeting information. KDEL-tagged recombinant human serum albumin, which is retrieved to the ER lumen in leaf cells, was deposited in prolamin aggregates within the vacuole of endosperm cells, most likely following the bulk of endogenous glutenins. Recombinant fungal phytase, a glycoprotein designed for secretion, was delivered to the same compartment, with no trace of the molecule in the apoplast. Glycan analysis revealed that this protein had passed through the Golgi. The localization of human serum albumin and phytase was compared to that of recombinant legumin, which contains structural targeting information directing it to the vacuole. Uniquely, legumin accumulated in the globulin inclusion bodies at the periphery of the prolamin bodies, suggesting a different mode of transport and/or aggregation. Our results demonstrate that recombinant proteins are deposited in an unexpected pattern within wheat endosperm cells, probably because of the unique storage properties of this tissue. Our data also confirm that recombinant proteins are invaluable tools for the analysis of protein trafficking in cereals.
This article has been cited by other articles:
|
|
|
|
 
T. Motoyama, N. Maruyama, Y. Amari, K. Kobayashi, H. Washida, T. Higasa, F. Takaiwa, and S. Utsumi
{alpha}' Subunit of soybean {beta}-conglycinin forms complex with rice glutelin via a disulphide bond in transgenic rice seeds
J. Exp. Bot.,
October 1, 2009;
60(14):
4015 - 4027.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Moeller, Q. Gan, and K. Wang
A bacterial signal peptide is functional in plants and directs proteins to the secretory pathway
J. Exp. Bot.,
August 1, 2009;
60(12):
3337 - 3352.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Tosi, M. Parker, C. S. Gritsch, R. Carzaniga, B. Martin, and P. R. Shewry
Trafficking of storage proteins in developing grain of wheat
J. Exp. Bot.,
March 1, 2009;
60(3):
979 - 991.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Saito, K. Kishida, K. Takata, H. Takahashi, T. Shimada, K. Tanaka, S. Morita, S. Satoh, and T. Masumura
A green fluorescent protein fused to rice prolamin forms protein body-like structures in transgenic rice
J. Exp. Bot.,
February 1, 2009;
60(2):
615 - 627.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Takaiwa, C. Sakuta, S.-K. Choi, Y. Tada, T. Motoyama, and S. Utsumi
Co-Expression of Soybean Glycinins A1aB1b and A3B4 Enhances Their Accumulation Levels in Transgenic Rice Seed
Plant Cell Physiol.,
October 1, 2008;
49(10):
1589 - 1599.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Drakakaki, S. Marcel, E. Arcalis, F. Altmann, P. Gonzalez-Melendi, R. Fischer, P. Christou, and E. Stoger
The Intracellular Fate of a Recombinant Protein Is Tissue Dependent
Plant Physiology,
June 1, 2006;
141(2):
578 - 586.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Vitale and E. Pedrazzini
Recombinant Pharmaceuticals from Plants: The Plant Endomembrane System as Bioreactor
Mol. Interv.,
August 1, 2005;
5(4):
216 - 225.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kawagoe, K. Suzuki, M. Tasaki, H. Yasuda, K. Akagi, E. Katoh, N. K. Nishizawa, M. Ogawa, and F. Takaiwa
The Critical Role of Disulfide Bond Formation in Protein Sorting in the Endosperm of Rice
PLANT CELL,
April 1, 2005;
17(4):
1141 - 1153.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
