Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on December 23, 2004; 10.1104/pp.104.050187

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
137/1/369    most recent
pp.104.050187v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miller, B.
Right arrow Articles by Bohlmann, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miller, B.
Right arrow Articles by Bohlmann, J.
Agricola
Right arrow Articles by Miller, B.
Right arrow Articles by Bohlmann, J.

Received ,
Accepted ,

Insect-Induced Conifer Defense. White Pine Weevil and Methyl Jasmonate Induce Traumatic Resinosis, de Novo Formed Volatile Emissions, and Accumulation of Terpenoid Synthase and Putative Octadecanoid Pathway Transcripts in Sitka Spruce

Barbara Miller , Lufiani L. Madilao , Steven Ralph , and Jörg Bohlmann *

Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3
Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3; Departments of Botany and Forest Science, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3

* Corresponding author; email: bohlmann{at}interchange.ubc.ca.

Stem-boring insects and methyl jasmonate (MeJA) are thought to induce similar complex chemical and anatomical defenses in conifers. To compare insect- and MeJA-induced terpenoid responses, we analyzed traumatic oleoresin mixtures, emissions of terpenoid volatiles, and expression of terpenoid synthase (TPS) genes in Sitka spruce (Picea sitchensis) following attack by white pine weevils (Pissodes strobi) or application of MeJA. Both insects and MeJA caused traumatic resin accumulation in stems, with more accumulation induced by the weevils. Weevil-induced terpenoid emission profiles were also more complex than emissions induced by MeJA. Weevil feeding caused a rapid release of a blend of monoterpene olefins, presumably by passive evaporation of resin compounds from stem feeding sites. These compounds were not found in MeJA-induced emissions. Both weevils and MeJA caused delayed, diurnal emissions of (-)-linalool, indicating induced de novo biosynthesis of this compound. TPS transcripts strongly increased in stems upon insect attack or MeJA treatment. Time courses and intensity of induced TPS transcripts were different for monoterpene synthases, sesquiterpene synthases, and diterpene synthases. Increased levels of weevil- and MeJA-induced TPS transcripts accompanied major changes in terpenoid accumulation in stems. Induced TPS expression profiles in needles were less complex than those in stems and matched induced de novo emissions of (-)-linalool. Overall, weevils and MeJA induced similar, but not identical, terpenoid defense responses in Sitka spruce. Findings of insect- and MeJA-induced accumulation of allene oxide synthase-like and allene oxide cyclase-like transcripts are discussed in the context of traumatic resinosis and induced volatile emissions in this gymnosperm system.




This article has been cited by other articles:


Home page
 J Exp BotHome page
M. Friedmann, S. G. Ralph, D. Aeschliman, J. Zhuang, K. Ritland, B. E. Ellis, J. Bohlmann, and C. J. Douglas
Microarray gene expression profiling of developmental transitions in Sitka spruce (Picea sitchensis) apical shoots
J. Exp. Bot., February 1, 2007; 58(3): 593 - 614.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. G. Ralph, J.W. Hudgins, S. Jancsik, V. R. Franceschi, and J. Bohlmann
Aminocyclopropane Carboxylic Acid Synthase Is a Regulated Step in Ethylene-Dependent Induced Conifer Defense. Full-Length cDNA Cloning of a Multigene Family, Differential Constitutive, and Wound- and Insect-Induced Expression, and Cellular and Subcellular Localization in Spruce and Douglas Fir
Plant Physiology, January 1, 2007; 143(1): 410 - 424.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Byun-McKay, K.-A. Godard, M. Toudefallah, D. M. Martin, R. Alfaro, J. King, J. Bohlmann, and A. L. Plant
Wound-Induced Terpene Synthase Gene Expression in Sitka Spruce That Exhibit Resistance or Susceptibility to Attack by the White Pine Weevil
Plant Physiology, March 1, 2006; 140(3): 1009 - 1021.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D.-K. Ro, G.-I. Arimura, S. Y. W. Lau, E. Piers, and J. Bohlmann
Loblolly pine abietadienol/abietadienal oxidase PtAO (CYP720B1) is a multifunctional, multisubstrate cytochrome P450 monooxygenase
PNAS, May 31, 2005; 102(22): 8060 - 8065.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2004 by the American Society of Plant Biologists