Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on April 22, 2005; 10.1104/pp.104.051144

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
138/1/255    most recent
pp.104.051144v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Underwood, B. A.
Right arrow Articles by Clark, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Underwood, B. A.
Right arrow Articles by Clark, D. G.
Agricola
Right arrow Articles by Underwood, B. A.
Right arrow Articles by Clark, D. G.

Received August 4, 2004
Returned for revision February 1, 2005
Accepted February 1, 2005

Ethylene-Regulated Floral Volatile Synthesis in Petunia Corollas

Beverly A. Underwood , Denise M. Tieman , Kenichi Shibuya , Richard J. Dexter , Holly M. Loucas , Andrew J. Simkin , Charles A. Sims , Eric A. Schmelz , Harry J. Klee , and David G. Clark *

Department of Environmental Horticulture, University of Florida, Gainesville, Florida 32611
Department of Horticultural Sciences, University of Florida, Gainesville, Florida 32611
Department of Food Science and Human Nutrition, University of Florida, Gainesville, Florida 32611
Center of Medical, Agricultural, and Veterinary Entomology, United States Department of Agriculture-Agricultural Research Service, Gainesville, Florida 32608

* Corresponding author; email: geranium{at}ufl.edu.

In many flowering plants, such as petunia (Petunia x hybrida), ethylene produced in floral organs after pollination elicits a series of physiological and biochemical events, ultimately leading to senescence of petals and successful fertilization. Here, we demonstrate, using transgenic ethylene insensitive (44568) and Mitchell Diploid petunias, that multiple components of emission of volatile organic compounds (VOCs) are regulated by ethylene. Expression of benzoic acid/salicylic acid carboxyl methyltransferase (PhBSMT1 and 2) mRNA is temporally and spatially down-regulated in floral organs in a manner consistent with current models for postpollination ethylene synthesis in petunia corollas. Emission of methylbenzoate and other VOCs after pollination and exogenous ethylene treatment parallels a reduction in PhBSMT1 and 2 mRNA levels. Under cyclic light conditions (day/night), PhBSMT mRNA levels are rhythmic and precede emission of methylbenzoate by approximately 6 h. When shifted into constant dark or light conditions, PhBSMT mRNA levels and subsequent methylbenzoate emission correspondingly decrease or increase to minimum or maximum levels observed during normal conditions, thus suggesting that light may be a more critical influence on cyclic emission of methylbenzoate than a circadian clock. Transgenic PhBSMT RNAi flowers with reduced PhBSMT mRNA levels show a 75% to 99% decrease in methylbenzoate emission, with minimal changes in other petunia VOCs. These results implicate PhBSMT1 and 2 as genes responsible for synthesis of methylbenzoate in petunia.




This article has been cited by other articles:


Home page
 J Exp BotHome page
R. J. Dexter, J. C. Verdonk, B. A. Underwood, K. Shibuya, E. A. Schmeltz, and D. G. Clark
Tissue-specific PhBPBT expression is differentially regulated in response to endogenous ethylene
J. Exp. Bot., February 5, 2008; (2008) erm337v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Spitzer, M. M. B. Zvi, M. Ovadis, E. Marhevka, O. Barkai, O. Edelbaum, I. Marton, T. Masci, M. Alon, S. Morin, et al.
Reverse Genetics of Floral Scent: Application of Tobacco Rattle Virus-Based Gene Silencing in Petunia
Plant Physiology, December 1, 2007; 145(4): 1241 - 1250.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
J. J. Weber and C. Goodwillie
Timing of self-compatibility, flower longevity, and potential for male outcross success in Leptosiphon jepsonii (Polemoniaceae)
Am. J. Botany, August 1, 2007; 94(8): 1338 - 1343.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2005 by the American Society of Plant Biologists