Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on November 19, 2004; 10.1104/pp.104.051623

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
136/4/3956    most recent
pp.104.051623v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (49)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Van Damme, D.
Right arrow Articles by Geelen, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van Damme, D.
Right arrow Articles by Geelen, D.
Agricola
Right arrow Articles by Van Damme, D.
Right arrow Articles by Geelen, D.

Received August 22, 2004
Returned for revision October 11, 2004
Accepted October 11, 2004

In Vivo Dynamics and Differential Microtubule-Binding Activities of MAP65 Proteins

Daniël Van Damme , Kris Van Poucke , Emmanuel Boutant , Christophe Ritzenthaler , Dirk Inzé *, and Danny Geelen

Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, B-9052 Ghent, Belgium
Institut de Biologie Moleculaire des Plantes, F-67084 Strasbourg cedex, France

* Corresponding author; email: dirk.inze{at}psb.ugent.be.

Plant cells produce different microtubule arrays that are essential for cell division and morphogenesis without equivalent in other eukaryotes. Microtubule-associated proteins influence the behavior of microtubules that is presumed to culminate into transitions from one array to another. We analyzed the microtubule-binding properties of three Arabidopsis (Arabidopsis thaliana) members, AtMAP65-1, AtMAP65-4, and AtMAP65-5, in live cells using laser scanning confocal microscopy. Depending on the overall organization of the cortical array, AtMAP65-1-GFP (green fluorescent protein) and AtMAP65-5-GFP associated with a subset of microtubules. In cells containing both coaligned and oblique microtubules, AtMAP65-1-GFP and AtMAP65-5-GFP tended to be associated with the coaligned microtubules. Cortical microtubules labeled with AtMAP65-1-GFP and AtMAP65-5-GFP appeared as thick bundles and showed more resistance to microtubule-destabilizing drugs. The polymerization rates of AtMAP65-1-GFP and AtMAP65-5-GFP microtubules were similar to those of tubulin-GFP marked microtubules but were different from AtEB1a-GFP, a microtubule plus-end-binding EB1-like protein that stimulated polymerization. By contrast, depolymerization rates of AtMAP65-1-GFP- and AtMAP65-5-GFP-labeled microtubules were reduced. AtMAP65-1-GFP associated with polymerizing microtubules within a bundle, and with fixed microtubule termini, suggesting that AtMAP65-1's function is to bundle and stabilize adjacent microtubules of the cortex. Polymerization within a bundle took place in either direction so that bundling occurred between parallel or antiparallel aligned microtubules. AtMAP65-4-GFP did not label cortical microtubules or the preprophase band, despite continuous expression driven by the 35S promoter, and its subcellular localization was restricted to microtubules that rearranged to form a spindle and the polar sides of the spindle proper. The expression of AtMAP65-4 peaked at mitosis, in agreement with a function related to spindle formation, whereas AtMAP65-1 and AtMAP65-5 were expressed throughout the cell cycle.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
C. Hofmann, A. Niehl, A. Sambade, A. Steinmetz, and M. Heinlein
Inhibition of Tobacco Mosaic Virus Movement by Expression of an Actin-Binding Protein
Plant Physiology, April 1, 2009; 149(4): 1810 - 1823.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
L. Guo, C.-M. K. Ho, Z. Kong, Y.-R. J. Lee, Q. Qian, and B. Liu
Evaluating the microtubule cytoskeleton and its interacting proteins in monocots by mining the rice genome
Ann. Bot., February 1, 2009; 103(3): 387 - 402.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. Pignocchi, G. E. Minns, N. Nesi, R. Koumproglou, G. Kitsios, C. Benning, C. W. Lloyd, J. H. Doonan, and M. J Hills
ENDOSPERM DEFECTIVE1 Is a Novel Microtubule-Associated Protein Essential for Seed Development in Arabidopsis
PLANT CELL, January 1, 2009; 21(1): 90 - 105.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. P. Smertenko, D. Kaloriti, H.-Y. Chang, J. Fiserova, Z. Opatrny, and P. J. Hussey
The C-Terminal Variable Region Specifies the Dynamic Properties of Arabidopsis Microtubule-Associated Protein MAP65 Isotypes
PLANT CELL, December 1, 2008; 20(12): 3346 - 3358.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. C. Ambrose and G. O. Wasteneys
CLASP Modulates Microtubule-Cortex Interaction during Self-Organization of Acentrosomal Microtubules
Mol. Biol. Cell, November 1, 2008; 19(11): 4730 - 4737.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. Gaillard, E. Neumann, D. Van Damme, V. Stoppin-Mellet, C. Ebel, E. Barbier, D. Geelen, and M. Vantard
Two Microtubule-associated Proteins of Arabidopsis MAP65s Promote Antiparallel Microtubule Bundling
Mol. Biol. Cell, October 1, 2008; 19(10): 4534 - 4544.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Brandner, A. Sambade, E. Boutant, P. Didier, Y. Mely, C. Ritzenthaler, and M. Heinlein
Tobacco Mosaic Virus Movement Protein Interacts with Green Fluorescent Protein-Tagged Microtubule End-Binding Protein 1
Plant Physiology, June 1, 2008; 147(2): 611 - 623.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. A. Barton, M. Vantard, and R. L. Overall
Analysis of Cortical Arrays from Tradescantia virginiana at High Resolution Reveals Discrete Microtubule Subpopulations and Demonstrates That Confocal Images of Arrays Can Be Misleading
PLANT CELL, April 1, 2008; 20(4): 982 - 994.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
High-Resolution Imaging of Cortical Microtubule Arrays
PLANT CELL, April 1, 2008; 20(4): 817 - 819.
[Full Text] [PDF]

Home page
 Plant CellHome page
S. R. Bisgrove, Y.-R. J. Lee, B. Liu, N. T. Peters, and D. L. Kropf
The Microtubule Plus-End Binding Protein EB1 Functions in Root Responses to Touch and Gravity Signals in Arabidopsis
PLANT CELL, February 1, 2008; 20(2): 396 - 410.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. V. Korolev, H. Buschmann, J. H. Doonan, and C. W. Lloyd
AtMAP70-5, a divergent member of the MAP70 family of microtubule-associated proteins, is required for anisotropic cell growth in Arabidopsis
J. Cell Sci., July 1, 2007; 120(13): 2241 - 2247.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. Li, T. Mao, Z. Zhang, and M. Yuan
The AtMAP65-1 Cross-Bridge Between Microtubules is Formed by One Dimer
Plant Cell Physiol., June 1, 2007; 48(6): 866 - 874.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. Van Damme, S. Coutuer, R. De Rycke, F.-Y. Bouget, D. Inze, and D. Geelen
Somatic Cytokinesis and Pollen Maturation in Arabidopsis Depend on TPLATE, Which Has Domains Similar to Coat Proteins
PLANT CELL, December 1, 2006; 18(12): 3502 - 3518.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. Ashby, E. Boutant, M. Seemanpillai, A. Sambade, C. Ritzenthaler, and M. Heinlein
Tobacco mosaic virus movement protein functions as a structural microtubule-associated protein.
J. Virol., September 1, 2006; 80(17): 8329 - 8344.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. P. Smertenko, H.-Y. Chang, S. Sonobe, S. I. Fenyk, M. Weingartner, L. Bogre, and P. J. Hussey
Control of the AtMAP65-1 interaction with microtubules through the cell cycle
J. Cell Sci., August 1, 2006; 119(15): 3227 - 3237.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. Ferralli, J. Ashby, M. Fasler, V. Boyko, and M. Heinlein
Disruption of Microtubule Organization and Centrosome Function by Expression of Tobacco Mosaic Virus Movement Protein.
J. Virol., June 1, 2006; 80(12): 5807 - 5821.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M. Sasabe, T. Soyano, Y. Takahashi, S. Sonobe, H. Igarashi, T. J. Itoh, M. Hidaka, and Y. Machida
Phosphorylation of NtMAP65-1 by a MAP kinase down-regulates its activity of microtubule bundling and stimulates progression of cytokinesis of tobacco cells
Genes & Dev., April 15, 2006; 20(8): 1004 - 1014.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. Dixit, E. Chang, and R. Cyr
Establishment of Polarity during Organization of the Acentrosomal Plant Cortical Microtubule Array
Mol. Biol. Cell, March 1, 2006; 17(3): 1298 - 1305.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
G. Mao, H. Buschmann, J. H. Doonan, and C. W. Lloyd
The role of MAP65-1 in microtubule bundling during Zinnia tracheary element formation
J. Cell Sci., February 15, 2006; 119(4): 753 - 758.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Kawamura, R. Himmelspach, M. C. Rashbrooke, A. T. Whittington, K. R. Gale, D. A. Collings, and G. O. Wasteneys
MICROTUBULE ORGANIZATION 1 Regulates Structure and Function of Microtubule Arrays during Mitosis and Cytokinesis in the Arabidopsis Root
Plant Physiology, January 1, 2006; 140(1): 102 - 114.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Mao, L. Jin, H. Li, B. Liu, and M. Yuan
Two Microtubule-Associated Proteins of the Arabidopsis MAP65 Family Function Differently on Microtubules
Plant Physiology, June 1, 2005; 138(2): 654 - 662.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. E. Janson, T. G. Setty, A. Paoletti, and P.T. Tran
Efficient formation of bipolar microtubule bundles requires microtubule-bound {gamma}-tubulin complexes
J. Cell Biol., April 25, 2005; 169(2): 297 - 308.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. O. Wasteneys and Z. Yang
The Cytoskeleton Becomes Multidisciplinary
Plant Physiology, December 1, 2004; 136(4): 3853 - 3854.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. O. Wasteneys and Z. Yang
New Views on the Plant Cytoskeleton
Plant Physiology, December 1, 2004; 136(4): 3884 - 3891.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2004 by the American Society of Plant Biologists