Plant Physiology Preview
Published on December 23, 2004; 10.1104/pp.104.054312
Received October 4, 2004
Returned for revision November 11, 2004
Accepted November 15, 2004
Annotations and Functional Analyses of the Rice WRKY Gene Superfamily Reveal Positive and Negative Regulators of Abscisic Acid Signaling in Aleurone Cells
Zhen Xie , Zhong-Lin Zhang , Xiaolu Zou , Jie Huang , Paul Ruas , Daniel Thompson , and Qingxi J. Shen *
Department of Biological Sciences, University of Nevada, Las Vegas, Nevada 89154
* Corresponding author; email: jeffery.shen{at}ccmail.nevada.edu.
The WRKY proteins are a superfamily of regulators that control diverse developmental and physiological processes. This family was believed to be plant specific until the recent identification of WRKY genes in nonphotosynthetic eukaryotes. We have undertaken a comprehensive computational analysis of the rice (Oryza sativa) genomic sequences and predicted the structures of 81 OsWRKY genes, 48 of which are supported by full-length cDNA sequences. Eleven OsWRKY proteins contain two conserved WRKY domains, while the rest have only one. Phylogenetic analyses of the WRKY domain sequences provide support for the hypothesis that gene duplication of single- and two-domain WRKY genes, and loss of the WRKY domain, occurred in the evolutionary history of this gene family in rice. The phylogeny deduced from the WRKY domain peptide sequences is further supported by the position and phase of the intron in the regions encoding the WRKY domains. Analyses for chromosomal distributions reveal that 26% of the predicted OsWRKY genes are located on chromosome 1. Among the dozen genes tested, OsWRKY24, -51, -71, and -72 are induced by abscisic acid (ABA) in aleurone cells. Using a transient expression system, we have demonstrated that OsWRKY24 and -45 repress ABA induction of the HVA22 promoter- -glucuronidase construct, while OsWRKY72 and -77 synergistically interact with ABA to activate this reporter construct. This study provides a solid base for functional genomics studies of this important superfamily of regulatory genes in monocotyledonous plants and reveals a novel function for WRKY genes, i.e. mediating plant responses to ABA.
This article has been cited by other articles:
|
|
|
|
 
H.-R. Kim, K.-S. Chae, K.-H. Han, and D.-M. Han
The nsdC Gene Encoding a Putative C2H2-Type Transcription Factor Is a Key Activator of Sexual Development in Aspergillus nidulans
Genetics,
July 1, 2009;
182(3):
771 - 783.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Liu, A. Zhu, L. Chai, W. Zhou, K. Yu, J. Ding, J. Xu, and X. Deng
Transcriptome analysis of a spontaneous mutant in sweet orange [Citrus sinensis (L.) Osbeck] during fruit development
J. Exp. Bot.,
March 1, 2009;
60(3):
801 - 813.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Huang, G. Lu, Q. Zhao, X. Liu, and B. Han
Genome-Wide Analysis of Transposon Insertion Polymorphisms Reveals Intraspecific Variation in Cultivated Rice
Plant Physiology,
September 1, 2008;
148(1):
25 - 40.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Ramamoorthy, S.-Y. Jiang, N. Kumar, P. N. Venkatesh, and S. Ramachandran
A Comprehensive Transcriptional Profiling of the WRKY Gene Family in Rice Under Various Abiotic and Phytohormone Treatments
Plant Cell Physiol.,
June 1, 2008;
49(6):
865 - 879.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Peng, L. E. Bartley, X. Chen, C. Dardick, M. Chern, R. Ruan, P. E. Canlas, and P. C. Ronald
OsWRKY62 is a Negative Regulator of Basal and Xa21-Mediated Defense against Xanthomonas oryzae pv. oryzae in Rice
Mol Plant,
May 1, 2008;
1(3):
446 - 458.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Marchive, R. Mzid, L. Deluc, F. Barrieu, J. Pirrello, A. Gauthier, M.-F. Corio-Costet, F. Regad, B. Cailleteau, S. Hamdi, et al.
Isolation and characterization of a Vitis vinifera transcription factor, VvWRKY1, and its effect on responses to fungal pathogens in transgenic tobacco plants
J. Exp. Bot.,
June 1, 2007;
58(8):
1999 - 2010.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Shimono, S. Sugano, A. Nakayama, C.-J. Jiang, K. Ono, S. Toki, and H. Takatsuji
Rice WRKY45 Plays a Crucial Role in Benzothiadiazole-Inducible Blast Resistance
PLANT CELL,
June 1, 2007;
19(6):
2064 - 2076.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-R. Duan, J. Nan, Y.-H. Liang, P. Mao, L. Lu, L. Li, C. Wei, L. Lai, Y. Li, and X.-D. Su
DNA binding mechanism revealed by high resolution crystal structure of Arabidopsis thaliana WRKY1 protein
Nucleic Acids Res.,
February 28, 2007;
35(4):
1145 - 1154.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q.-H. Shen, Y. Saijo, S. Mauch, C. Biskup, S. Bieri, B. Keller, H. Seki, B. Ulker, I. E. Somssich, and P. Schulze-Lefert
Nuclear Activity of MLA Immune Receptors Links Isolate-Specific and Basal Disease-Resistance Responses
Science,
February 23, 2007;
315(5815):
1098 - 1103.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-h. Lee, D. A. Henderson, and J.-K. Zhu
The Arabidopsis Cold-Responsive Transcriptome and Its Regulation by ICE1
PLANT CELL,
November 1, 2005;
17(11):
3155 - 3175.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
