Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on March 4, 2005; 10.1104/pp.104.055277

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
137/4/1250    most recent
pp.104.055277v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (31)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Imin, N.
Right arrow Articles by Rolfe, B. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Imin, N.
Right arrow Articles by Rolfe, B. G.
Agricola
Right arrow Articles by Imin, N.
Right arrow Articles by Rolfe, B. G.

Received October 19, 2004
Returned for revision January 6, 2005
Accepted January 9, 2005

Proteomic Analysis of Somatic Embryogenesis in Medicago truncatula. Explant Cultures Grown under 6-Benzylaminopurine and 1-Naphthaleneacetic Acid Treatments

Nijat Imin , Mahira Nizamidin , Daniel Daniher , Kim E. Nolan , Ray J. Rose , and Barry G. Rolfe *

Australian Research Council Centre of Excellence for Integrative Legume Research, Genomic Interactions Group, Research School of Biological Sciences, Australian National University, Canberra City, Australian Capital Territory, 2601, Australia
University of Newcastle and Australian Research Council Centre of Excellence for Integrative Legume Research, School of Environmental and Life Sciences, University Drive, Callaghan, New South Wales, 2308, Australia

* Corresponding author; email: rolfe{at}rsbs.anu.edu.au.

The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared proteomes of tissue cultures from leaf explants of these two lines. Both 2HA and Jemalong explants were grown on media containing the auxin 1-naphthaleneacetic acid and the cytokinin 6-benzylaminopurine. Proteins were extracted from the cultures at different time points (2, 5, and 8 weeks), separated by two-dimensional gel electrophoresis, and detected by silver staining. More than 2,000 proteins could be reproducibly resolved and detected on each gel. Statistical analysis showed that 54 protein spots were significantly (P < 0.05) changed in expression (accumulation) during the 8 weeks of culture, and most of these spots were extracted from colloidal Coomassie-stained two-dimensional gel electrophoresis gels and were subjected to matrix-assisted laser desorption ionization time-of-flight mass spectrometry or liquid chromatography-tandem mass spectrometry analysis. Using a publicly available expressed sequence tag database and the Mascot search engine, we were able to identify 16 differentially expressed proteins. More than 60% of the differentially expressed protein spots had very different patterns of gene expression between 2HA and Jemalong during the 8 weeks of culture.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
F. R. Mantiri, S. Kurdyukov, D. P. Lohar, N. Sharopova, N. A. Saeed, X.-D. Wang, K. A. VandenBosch, and R. J. Rose
The Transcription Factor MtSERF1 of the ERF Subfamily Identified by Transcriptional Profiling Is Required for Somatic Embryogenesis Induced by Auxin Plus Cytokinin in Medicago truncatula
Plant Physiology, April 1, 2008; 146(4): 1622 - 1636.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
N. Imin, M. Nizamidin, T. Wu, and B. G. Rolfe
Factors involved in root formation in Medicago truncatula
J. Exp. Bot., February 1, 2007; 58(3): 439 - 451.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. J. Rose, X.-D. Wang, K. E. Nolan, and B. G. Rolfe
Root meristems in Medicago truncatula tissue culture arise from vascular-derived procambial-like cells in a process regulated by ethylene
J. Exp. Bot., July 1, 2006; 57(10): 2227 - 2235.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
N. Imin, T. Kerim, J. J. Weinman, and B. G. Rolfe
Low Temperature Treatment at the Young Microspore Stage Induces Protein Changes in Rice Anthers
Mol. Cell. Proteomics, February 1, 2006; 5(2): 274 - 292.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Fujiwara, K. Umemura, T. Kawasaki, and K. Shimamoto
Proteomics of Rac GTPase Signaling Reveals Its Predominant Role in Elicitor-Induced Defense Response of Cultured Rice Cells
Plant Physiology, February 1, 2006; 140(2): 734 - 745.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2005 by the American Society of Plant Biologists