Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on March 25, 2005; 10.1104/pp.104.055939

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
137/4/1456    most recent
pp.104.055939v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McAlvin, C. B.
Right arrow Articles by Stacey, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McAlvin, C. B.
Right arrow Articles by Stacey, G.
Agricola
Right arrow Articles by McAlvin, C. B.
Right arrow Articles by Stacey, G.

Received November 2, 2004
Returned for revision February 1, 2005
Accepted February 3, 2005

Transgenic Expression of the Soybean Apyrase in Lotus japonicus Enhances Nodulation

Crystal B. McAlvin and Gary Stacey *

Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996
Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996; National Center for Soybean Biotechnology, Divisions of Plant Science and Biochemistry, University of Missouri, Columbia, Missouri 65211

* Corresponding author; email: staceyg{at}missouri.edu.

The soybean apyrase, GS52, was previously characterized as an early nodulin that is expressed in roots and localized to the plasma membrane. Transgenic Lotus japonicus plants were constructed constitutively expressing the GS52 apyrase. Segregation and Southern-blot analysis identified four single-copy sense lines, several double-copy sense lines, and one double-copy antisense line for further analysis. The single- and double-copy sense gs52 L. japonicus lines had enhanced nodulation that correlated with expression of the transgene. The sense transgenic lines were also found to have increased infection thread formation and enhanced infection zone length when infected by Mesorhizobium loti, the natural symbiont of L. japonicus. The data presented show that expression of the GS52 apyrase can enhance nodulation in L. japonicus and points to an important role for this group of enzymes in nodulation.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
D. Riewe, L. Grosman, A. R. Fernie, C. Wucke, and P. Geigenberger
The Potato-Specific Apyrase Is Apoplastically Localized and Has Influence on Gene Expression, Growth, and Development
Plant Physiology, July 1, 2008; 147(3): 1092 - 1109.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S.-Y. Kim, M. Sivaguru, and G. Stacey
Extracellular ATP in Plants. Visualization, Localization, and Analysis of Physiological Significance in Growth and Signaling
Plant Physiology, November 1, 2006; 142(3): 984 - 992.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
N. Takeda, S. Okamoto, M. Hayashi, and Y. Murooka
Expression of LjENOD40 Genes in Response to Symbiotic and Non-symbiotic Signals: LjENOD40-1 and LjENOD40-2 are Differentially Regulated in Lotus japonicus
Plant Cell Physiol., August 1, 2005; 46(8): 1291 - 1298.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2005 by the American Society of Plant Biologists