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Published on April 22, 2005; 10.1104/pp.104.056010

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Received November 3, 2004
Returned for revision February 15, 2005
Accepted March 2, 2005

Antisense Suppression of a (+)-{delta}-Cadinene Synthase Gene in Cotton Prevents the Induction of This Defense Response Gene during Bacterial Blight Infection But Not Its Constitutive Expression

Belinda J. Townsend , Andrew Poole , Christopher J. Blake , and Danny J. Llewellyn *

Commonwealth Scientific and Industrial Research Organisation-Plant Industry, Canberra, Australian Capital Territory 2601, Australia; Department of Biochemistry and Molecular Biology, Australian National University, Canberra, Australian Capital Territory 0200, Australia
Commonwealth Scientific and Industrial Research Organisation-Plant Industry, Canberra, Australian Capital Territory 2601, Australia
Research School of Chemistry, Australian National University, Canberra, Australian Capital Territory 0200, Australia

* Corresponding author; email: danny.llewellyn{at}csiro.au.

In cotton (Gossypium hirsutum) the enzyme (+)-{delta}-cadinene synthase (CDNS) catalyzes the first committed step in the biosynthesis of cadinane-type sesquiterpenes, such as gossypol, that provide constitutive and inducible protection against pests and diseases. A cotton cDNA clone encoding CDNS (cdn1-C4) was isolated from developing embryos and functionally characterized. Southern analysis showed that CDNS genes belong to a large multigene family, of which five genomic clones were studied, including three pseudogenes and one gene that may represent another subfamily of CDNS. CDNS expression was shown to be induced in cotton infected with either the bacterial blight or verticillium wilt pathogens. Constructs for the constitutive or seed-specific antisense suppression of cdn1-C4 were introduced into cotton by Agrobacterium-mediated transformation. Gossypol levels were not reduced in the seeds of transformants with either construct, nor was the induction of CDNS expression affected in stems of the constitutive antisense plants infected with Verticillium dahliae Kleb. However, the induction of CDNS mRNA and protein in response to bacterial blight infection of cotyledons was completely blocked in the constitutive antisense plants. These results suggest that cdn1-C4 may be involved specifically in the bacterial blight response and that the CDNS multigene family comprises a complex set of genes differing in their temporal and spatial regulation and responsible for different branches of the cotton sesquiterpene pathway.




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From the Cover: Engineering cottonseed for use in human nutrition by tissue-specific reduction of toxic gossypol
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[Abstract] [Full Text] [PDF]


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