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Published on February 25, 2005; 10.1104/pp.104.057646

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Received December 2, 2004
Returned for revision January 11, 2005
Accepted January 11, 2005

A Novel Dwarfing Mutation in a Green Revolution Gene from Brassica rapa

Amorntip Muangprom , Stephen G. Thomas , Tai-ping Sun *, and Thomas C. Osborn

Department of Agronomy, University of Wisconsin, Madison, Wisconsin 53706; National Center for Genetic Engineering and Biotechnology, Klong Luang, Pathumthani 12120, Thailand
Department of Biology, Duke University, Durham, North Carolina 27708
Department of Agronomy, University of Wisconsin, Madison, Wisconsin 53706

* Corresponding author; email: tps{at}duke.edu.

Mutations in the biosynthesis or signaling pathways of gibberellin (GA) can cause dwarfing phenotypes in plants, and the use of such mutations in plant breeding was a major factor in the success of the Green Revolution. DELLA proteins are GA signaling repressors whose functions are conserved in different plant species. Recent studies show that GA promotes stem growth by causing degradation of DELLA proteins via the ubiquitin-proteasome pathway. The most widely utilized dwarfing alleles in wheat (Triticum aestivum; e.g. Rht-B1b and Rht-D1b) encode GA-resistant forms of a DELLA protein that function as dominant and constitutively active repressors of stem growth. All of the previously identified dominant DELLA repressors from several plant species contain N-terminal mutations. Here we report on a novel dwarf mutant from Brassica rapa (Brrga1-d) that is caused by substitution of a conserved amino acid in the C-terminal domain of a DELLA protein. Brrga1-d, like N-terminal DELLA mutants, retains its repressor function and accumulates to high levels, even in the presence of GA. However, unlike wild-type and N-terminal DELLA mutants, Brrga1-d does not interact with a protein component required for degradation, suggesting that the mutated amino acid causes dwarfism by preventing an interaction needed for its degradation. This novel mutation confers nondeleterious dwarf phenotypes when transferred to Arabidopsis (Arabidopsis thaliana) and oilseed (B. napus), indicating its potential usefulness in other crop species.




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