Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on May 20, 2005; 10.1104/pp.104.057935

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
138/2/744    most recent
pp.104.057935v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (115)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pina, C.
Right arrow Articles by Becker, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pina, C.
Right arrow Articles by Becker, J. D.
Agricola
Right arrow Articles by Pina, C.
Right arrow Articles by Becker, J. D.

Received December 8, 2004
Returned for revision February 2, 2005
Accepted February 3, 2005

Gene Family Analysis of the Arabidopsis Pollen Transcriptome Reveals Biological Implications for Cell Growth, Division Control, and Gene Expression Regulation

Cristina Pina , Francisco Pinto , José A. Feijó *, and Jörg D. Becker

Centro de Biologia do Desenvolvimento, Instituto Gulbenkian de Ciência, PT-2780-156 Oeiras, Portugal
Biomathematics Group, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, PT-2780-156 Oeiras, Portugal
Centro de Biologia do Desenvolvimento, Instituto Gulbenkian de Ciência, PT-2780-156 Oeiras, Portugal; Faculdade de Ciências, Departamento de Biologia Vegetal, Universidade de Lisboa, Campo Grande, PT-1749-016 Lisbon, Portugal

* Corresponding author; email: jfeijo{at}fc.ul.pt.

Upon germination, pollen forms a tube that elongates dramatically through female tissues to reach and fertilize ovules. While essential for the life cycle of higher plants, the genetic basis underlying most of the process is not well understood. We previously used a combination of flow cytometry sorting of viable hydrated pollen grains and GeneChip array analysis of one-third of the Arabidopsis (Arabidopsis thaliana) genome to define a first overview of the pollen transcriptome. We now extend that study to approximately 80% of the genome of Arabidopsis by using Affymetrix Arabidopsis ATH1 arrays and perform comparative analysis of gene family and gene ontology representation in the transcriptome of pollen and vegetative tissues. Pollen grains have a smaller and overall unique transcriptome (6,587 genes expressed) with greater proportions of selectively expressed (11%) and enriched (26%) genes than any vegetative tissue. Relative gene ontology category representations in pollen and vegetative tissues reveal a functional skew of the pollen transcriptome toward signaling, vesicle transport, and the cytoskeleton, suggestive of a commitment to germination and tube growth. Cell cycle analysis reveals an accumulation of G2/M-associated factors that may play a role in the first mitotic division of the zygote. Despite the relative underrepresentation of transcription-associated transcripts, nonclassical MADS box genes emerge as a class with putative unique roles in pollen. The singularity of gene expression control in mature pollen grains is further highlighted by the apparent absence of small RNA pathway components.




This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
L. Huang, Y. Ye, Y. Zhang, A. Zhang, T. Liu, and J. Cao
BcMF9, a novel polygalacturonase gene, is required for both Brassica campestris intine and exine formation
Ann. Bot., October 8, 2009; (2009) mcp244v1.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Boisson-Dernier, S. Roy, K. Kritsas, M. A. Grobei, M. Jaciubek, J. I. Schroeder, and U. Grossniklaus
Disruption of the pollen-expressed FERONIA homologs ANXUR1 and ANXUR2 triggers pollen tube discharge
Development, October 1, 2009; 136(19): 3279 - 3288.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
M. A. Grobei, E. Qeli, E. Brunner, H. Rehrauer, R. Zhang, B. Roschitzki, K. Basler, C. H. Ahrens, and U. Grossniklaus
Deterministic protein inference for shotgun proteomics data provides new insights into Arabidopsis pollen development and function
Genome Res., October 1, 2009; 19(10): 1786 - 1800.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
S. Wolf, G. Mouille, and J. Pelloux
Homogalacturonan Methyl-Esterification and Plant Development
Mol Plant, September 1, 2009; 2(5): 851 - 860.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Brownfield, S. Hafidh, A. Durbarry, H. Khatab, A. Sidorova, P. Doerner, and D. Twell
Arabidopsis DUO POLLEN3 Is a Key Regulator of Male Germline Development and Embryogenesis
PLANT CELL, July 1, 2009; 21(7): 1940 - 1956.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
R. Grant-Downton, S. Hafidh, D. Twell, and H. G. Dickinson
Small RNA Pathways Are Present and Functional in the Angiosperm Male Gametophyte
Mol Plant, May 1, 2009; 2(3): 500 - 512.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. A. Reichler, J. Torres, A. L. Rivera, V. A. Cintolesi, G. Clark, and S. J. Roux
Intersection of two signalling pathways: extracellular nucleotides regulate pollen germination and pollen tube growth via nitric oxide
J. Exp. Bot., May 1, 2009; 60(7): 2129 - 2138.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Borg, L. Brownfield, and D. Twell
Male gametophyte development: a molecular perspective
J. Exp. Bot., April 1, 2009; 60(5): 1465 - 1478.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. C. Boavida, B. Shuai, H.-J. Yu, G. C. Pagnussat, V. Sundaresan, and S. McCormick
A Collection of Ds Insertional Mutants Associated With Defects in Male Gametophyte Development and Function in Arabidopsis thaliana
Genetics, April 1, 2009; 181(4): 1369 - 1385.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. J. Adamczyk and D. E. Fernandez
MIKC* MADS Domain Heterodimers Are Required for Pollen Maturation and Tube Growth in Arabidopsis
Plant Physiology, April 1, 2009; 149(4): 1713 - 1723.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
R.A. Martienssen, A. Kloc, R.K. Slotkin, and M. Tanurdzic
Epigenetic Inheritance and Reprogramming in Plants and Fission Yeast
Cold Spring Harb Symp Quant Biol, March 27, 2009; (2009) sqb.2008.73.062v1.
[Abstract] [PDF]

Home page
 Plant Physiol.Home page
I. Dangoor, H. Peled-Zehavi, A. Levitan, O. Pasand, and A. Danon
A Small Family of Chloroplast Atypical Thioredoxins
Plant Physiology, March 1, 2009; 149(3): 1240 - 1250.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. L. Szumlanski and E. Nielsen
The Rab GTPase RabA4d Regulates Pollen Tube Tip Growth in Arabidopsis thaliana
PLANT CELL, February 1, 2009; 21(2): 526 - 544.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J.-L. Gallois, A. Guyon-Debast, A. Lecureuil, D. Vezon, V. Carpentier, S. Bonhomme, and P. Guerche
The Arabidopsis Proteasome RPT5 Subunits Are Essential for Gametophyte Development and Show Accession-Dependent Redundancy
PLANT CELL, February 1, 2009; 21(2): 442 - 459.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Chen, X. Wu, Y. Chen, X. Li, M. Huang, M. Zheng, F. Baluska, J. Samaj, and J. Lin
Combined Proteomic and Cytological Analysis of Ca2+-Calmodulin Regulation in Picea meyeri Pollen Tube Growth
Plant Physiology, February 1, 2009; 149(2): 1111 - 1126.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Huang, J. Cao, A. Zhang, Y. Ye, Y. Zhang, and T. Liu
The polygalacturonase gene BcMF2 from Brassica campestris is associated with intine development
J. Exp. Bot., January 1, 2009; 60(1): 301 - 313.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
L. Yuan, L. Graff, D. Loque, S. Kojima, Y. N. Tsuchiya, H. Takahashi, and N. von Wiren
AtAMT1;4, a Pollen-Specific High-Affinity Ammonium Transporter of the Plasma Membrane in Arabidopsis
Plant Cell Physiol., January 1, 2009; 50(1): 13 - 25.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. Lycett
The role of Rab GTPases in cell wall metabolism
J. Exp. Bot., November 1, 2008; 59(15): 4061 - 4074.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. Blanvillain, L. C. Boavida, S. McCormick, and D. W. Ow
EXPORTIN1 Genes Are Essential for Development and Function of the Gametophytes in Arabidopsis thaliana
Genetics, November 1, 2008; 180(3): 1493 - 1500.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Wang, W.-Z. Zhang, L.-F. Song, J.-J. Zou, Z. Su, and W.-H. Wu
Transcriptome Analyses Show Changes in Gene Expression to Accompany Pollen Germination and Tube Growth in Arabidopsis
Plant Physiology, November 1, 2008; 148(3): 1201 - 1211.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. J. Jakoby, D. Falkenhan, M. T. Mader, G. Brininstool, E. Wischnitzki, N. Platz, A. Hudson, M. Hulskamp, J. Larkin, and A. Schnittger
Transcriptional Profiling of Mature Arabidopsis Trichomes Reveals That NOECK Encodes the MIXTA-Like Transcriptional Regulator MYB106
Plant Physiology, November 1, 2008; 148(3): 1583 - 1602.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Mihara, T. Itoh, and T. Izawa
In Silico Identification of Short Nucleotide Sequences Associated with Gene Expression of Pollen Development in Rice
Plant Cell Physiol., October 1, 2008; 49(10): 1451 - 1464.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Y. Komarova, K. Thor, A. Gubler, S. Meier, D. Dietrich, A. Weichert, M. Suter Grotemeyer, M. Tegeder, and D. Rentsch
AtPTR1 and AtPTR5 Transport Dipeptides in Planta
Plant Physiology, October 1, 2008; 148(2): 856 - 869.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Borges, G. Gomes, R. Gardner, N. Moreno, S. McCormick, J. A. Feijo, and J. D. Becker
Comparative Transcriptomics of Arabidopsis Sperm Cells
Plant Physiology, October 1, 2008; 148(2): 1168 - 1181.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
Y. Onodera, K. Nakagawa, J. R. Haag, D. Pikaard, T. Mikami, T. Ream, Y. Ito, and C. S. Pikaard
Sex-Biased Lethality or Transmission of Defective Transcription Machinery in Arabidopsis
Genetics, September 1, 2008; 180(1): 207 - 218.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
A. Krishnan and A. Pereira
Integrative approaches for mining transcriptional regulatory programs in Arabidopsis
Brief Funct Genomic Proteomic, July 16, 2008; (2008) eln035v1.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
A. M. Prado, R. Colaco, N. Moreno, A. C. Silva, and J. A. Feijo
Targeting of Pollen Tubes to Ovules Is Dependent on Nitric Oxide (NO) Signaling
Mol Plant, July 1, 2008; 1(4): 703 - 714.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Zhang and S. McCormick
A distinct mechanism regulating a pollen-specific guanine nucleotide exchange factor for the small GTPase Rop in Arabidopsis thaliana
PNAS, November 20, 2007; 104(47): 18830 - 18835.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
J. D. Becker and J. A. Feijo
How Many Genes are Needed to Make a Pollen Tube? Lessons from Transcriptomics
Ann. Bot., November 1, 2007; 100(6): 1117 - 1123.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Alves-Ferreira, F. Wellmer, A. Banhara, V. Kumar, J. L. Riechmann, and E. M. Meyerowitz
Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development
Plant Physiology, November 1, 2007; 145(3): 747 - 762.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
I. S. Sheoran, A. R. S. Ross, D. J. H. Olson, and V. K. Sawhney
Proteomic analysis of tomato (Solanum lycopersicum, formerly hycopersicon esculentum) pollen
J. Exp. Bot., October 5, 2007; (2007) erm199v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Xing and S. Zachgo
Pollen Lethality: A Phenomenon in Arabidopsis RNA Interference Plants
Plant Physiology, October 1, 2007; 145(2): 330 - 333.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Frietsch, Y.-F. Wang, C. Sladek, L. R. Poulsen, S. M. Romanowsky, J. I. Schroeder, and J. F. Harper
A cyclic nucleotide-gated channel is essential for polarized tip growth of pollen
PNAS, September 4, 2007; 104(36): 14531 - 14536.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. K. Grennan
An Analysis of the Arabidopsis Pollen Transcriptome
Plant Physiology, September 1, 2007; 145(1): 3 - 4.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. R. Malik, F. Wang, J. M. Dirpaul, N. Zhou, P. L. Polowick, A. M.R. Ferrie, and J. E. Krochko
Transcript Profiling and Identification of Molecular Markers for Early Microspore Embryogenesis in Brassica napus
Plant Physiology, May 1, 2007; 144(1): 134 - 154.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Joosen, J. Cordewener, E. D. J. Supena, O. Vorst, M. Lammers, C. Maliepaard, T. Zeilmaker, B. Miki, T. America, J. Custers, et al.
Combined Transcriptome and Proteome Analysis Identifies Pathways and Markers Associated with the Establishment of Rapeseed Microspore-Derived Embryo Development
Plant Physiology, May 1, 2007; 144(1): 155 - 172.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
V. Lionetti, A. Raiola, L. Camardella, A. Giovane, N. Obel, M. Pauly, F. Favaron, F. Cervone, and D. Bellincampi
Overexpression of Pectin Methylesterase Inhibitors in Arabidopsis Restricts Fungal Infection by Botrytis cinerea
Plant Physiology, April 1, 2007; 143(4): 1871 - 1880.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Aluri and M. Buttner
Identification and functional expression of the Arabidopsis thaliana vacuolar glucose transporter 1 and its role in seed germination and flowering
PNAS, February 13, 2007; 104(7): 2537 - 2542.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
S. Dai, T. Chen, K. Chong, Y. Xue, S. Liu, and T. Wang
Proteomics Identification of Differentially Expressed Proteins Associated with Pollen Germination and Tube Growth Reveals Characteristics of Germinated Oryza sativa Pollen
Mol. Cell. Proteomics, February 1, 2007; 6(2): 207 - 230.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
T. J. Bushart and S. J. Roux
Conserved Features of Germination and Polarized Cell Growth: A Few Insights from a Pollen-Fern Spore Comparison
Ann. Bot., January 1, 2007; 99(1): 9 - 17.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. Verelst, H. Saedler, and T. Munster
MIKC* MADS-Protein Complexes Bind Motifs Enriched in the Proximal Region of Late Pollen-Specific Arabidopsis Promoters
Plant Physiology, January 1, 2007; 143(1): 447 - 460.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Drakakaki, O. Zabotina, I. Delgado, S. Robert, K. Keegstra, and N. Raikhel
Arabidopsis Reversibly Glycosylated Polypeptides 1 and 2 Are Essential for Pollen Development
Plant Physiology, December 1, 2006; 142(4): 1480 - 1492.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. M. Brady, T. A. Long, and P. N. Benfey
Unraveling the dynamic transcriptome.
PLANT CELL, September 1, 2006; 18(9): 2101 - 2111.
[Full Text] [PDF]

Home page
 Plant CellHome page
P. E. Dowd, S. Coursol, A. L. Skirpan, T.-h. Kao, and S. Gilroy
Petunia Phospholipase C1 Is Involved in Pollen Tube Growth
PLANT CELL, June 1, 2006; 18(6): 1438 - 1453.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
D. R. Hoen, K. C. Park, N. Elrouby, Z. Yu, N. Mohabir, R. K. Cowan, and T. E. Bureau
Transposon-Mediated Expansion and Diversification of a Family of ULP-like Genes
Mol. Biol. Evol., June 1, 2006; 23(6): 1254 - 1268.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Lemaitre and M. Hodges
Expression Analysis of Arabidopsis thaliana NAD-dependent Isocitrate Dehydrogenase Genes Shows the Presence of a Functional Subunit That Is Mainly Expressed in the Pollen and Absent from Vegetative Organs
Plant Cell Physiol., May 1, 2006; 47(5): 634 - 643.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. W. Bock, D. Honys, J. M. Ward, S. Padmanaban, E. P. Nawrocki, K. D. Hirschi, D. Twell, and H. Sze
Integrating Membrane Transport with Male Gametophyte Development and Function through Transcriptomics.
Plant Physiology, April 1, 2006; 140(4): 1151 - 1168.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Bosch and P. K. Hepler
Pectin Methylesterases and Pectin Dynamics in Pollen Tubes
PLANT CELL, December 1, 2005; 17(12): 3219 - 3226.
[Full Text] [PDF]

Home page
 Plant CellHome page
B. H.J. de Graaf, A. Y. Cheung, T. Andreyeva, K. Levasseur, M. Kieliszewski, and H.-m. Wu
Rab11 GTPase-Regulated Membrane Trafficking Is Crucial for Tip-Focused Pollen Tube Growth in Tobacco
PLANT CELL, September 1, 2005; 17(9): 2564 - 2579.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L.-z. Tao, A. Y. Cheung, C. Nibau, and H.-m. Wu
RAC GTPases in Tobacco and Arabidopsis Mediate Auxin-Induced Formation of Proteolytically Active Nuclear Protein Bodies That Contain AUX/IAA Proteins
PLANT CELL, August 1, 2005; 17(8): 2369 - 2383.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Bosch, A. Y. Cheung, and P. K. Hepler
Pectin Methylesterase, a Regulator of Pollen Tube Growth
Plant Physiology, July 1, 2005; 138(3): 1334 - 1346.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2005 by the American Society of Plant Biologists