Plant Physiology Preview
Published on April 15, 2005; 10.1104/pp.104.059147
Received December 29, 2004
Returned for revision February 1, 2005
Accepted February 1, 2005
Arabidopsis CBF3/DREB1A and ABF3 in Transgenic Rice Increased Tolerance to Abiotic Stress without Stunting Growth
Se-Jun Oh , Sang Ik Song , Youn Shic Kim , Hyun-Jun Jang , Soo Young Kim , Minjeong Kim , Yeon-Ki Kim , Baek Hie Nahm , and Ju-Kon Kim *
Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea
Genomics and Genetics Institute, GreenGene Biotech, Yongin 449-728, Korea
Kumho Life and Environmental Science Laboratory, Korea Kumho Petrochemical, Kwangju 500-712, Korea
Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea; Genomics and Genetics Institute, GreenGene Biotech, Yongin 449-728, Korea
* Corresponding author; email: jukon{at}bio.mju.ac.kr.
Rice (Oryza sativa), a monocotyledonous plant that does not cold acclimate, has evolved differently from Arabidopsis (Arabidopsis thaliana), which cold acclimates. To understand the stress response of rice in comparison with that of Arabidopsis, we developed transgenic rice plants that constitutively expressed CBF3/DREB1A (CBF3) and ABF3, Arabidopsis genes that function in abscisic acid-independent and abscisic acid-dependent stress-response pathways, respectively. CBF3 in transgenic rice elevated tolerance to drought and high salinity, and produced relatively low levels of tolerance to low-temperature exposure. These data were in direct contrast to CBF3 in Arabidopsis, which is known to function primarily to enhance freezing tolerance. ABF3 in transgenic rice increased tolerance to drought stress alone. By using the 60 K Rice Whole Genome Microarray and RNA gel-blot analyses, we identified 12 and 7 target genes that were activated in transgenic rice plants by CBF3 and ABF3, respectively, which appear to render the corresponding plants acclimated for stress conditions. The target genes together with 13 and 27 additional genes are induced further upon exposure to drought stress, consequently making the transgenic plants more tolerant to stress conditions. Interestingly, our transgenic plants exhibited neither growth inhibition nor visible phenotypic alterations despite constitutive expression of the CBF3 or ABF3, unlike the results previously obtained from Arabidopsis where transgenic plants were stunted.
This article has been cited by other articles:
|
|
|
|
 
S.-J. Oh, Y. S. Kim, C.-W. Kwon, H. K. Park, J. S. Jeong, and J.-K. Kim
Overexpression of the Transcription Factor AP37 in Rice Improves Grain Yield under Drought Conditions
Plant Physiology,
July 1, 2009;
150(3):
1368 - 1379.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. J. M. Saibo, T. Lourenco, and M. M. Oliveira
Transcription factors and regulation of photosynthetic and related metabolism under environmental stresses
Ann. Bot.,
February 1, 2009;
103(4):
609 - 623.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-Z. Xiao, X. Chen, C.-B. Xiang, N. Tang, Q.-F. Zhang, and L.-Z. Xiong
Evaluation of Seven Function-Known Candidate Genes for their Effects on Improving Drought Resistance of Transgenic Rice under Field Conditions
Mol Plant,
January 1, 2009;
2(1):
73 - 83.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Nakashima, Y. Ito, and K. Yamaguchi-Shinozaki
Transcriptional Regulatory Networks in Response to Abiotic Stresses in Arabidopsis and Grasses
Plant Physiology,
January 1, 2009;
149(1):
88 - 95.
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Century, T. L. Reuber, and O. J. Ratcliffe
Regulating the Regulators: The Future Prospects for Transcription-Factor-Based Agricultural Biotechnology Products
Plant Physiology,
May 1, 2008;
147(1):
20 - 29.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.I.L Morison, N.R Baker, P.M Mullineaux, and W.J Davies
Improving water use in crop production
Phil Trans R Soc B,
February 12, 2008;
363(1491):
639 - 658.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Shukla, S. Raha, V. Tripathi, and D. Chattopadhyay
Expression of CAP2, an APETALA2-Family Transcription Factor from Chickpea, Enhances Growth and Tolerance to Dehydration and Salt Stress in Transgenic Tobacco
Plant Physiology,
September 1, 2006;
142(1):
113 - 123.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Ito, K. Katsura, K. Maruyama, T. Taji, M. Kobayashi, M. Seki, K. Shinozaki, and K. Yamaguchi-Shinozaki
Functional Analysis of Rice DREB1/CBF-type Transcription Factors Involved in Cold-responsive Gene Expression in Transgenic Rice
Plant Cell Physiol.,
January 1, 2006;
47(1):
141 - 153.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
