Plant Physiology Preview
Published on June 24, 2005; 10.1104/pp.105.059907
Received January 19, 2005
Returned for revision April 13, 2005
Accepted April 25, 2005
Fatty Acid Hydroperoxides and H2O2 in the Execution of Hypersensitive Cell Death in Tobacco Leaves
Jean-Luc Montillet , Sangpen Chamnongpol , Christine Rustérucci , James Dat , Brigitte van de Cotte , Jean-Pierre Agnel , Christine Battesti , Dirk Inzé , Frank Van Breusegem , and Christian Triantaphylidès *
Commissariat à l'Energie Atomique/Cadarache, Direction des Sciences du Vivant, Département d'Ecophysiologie Végétale et de Microbiologie, Laboratoire de Radiobiologie Végétale, F-13108 Saint-Paul-Lez-Durance cedex, France
Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, B-9052 Ghent, Belgium
* Corresponding author; email: ctriantaphylid{at}cea.fr.
We initially compared lipid peroxidation profiles in tobacco (Nicotiana tabacum) leaves during different cell death events. An upstream oxylipin assay was used to discriminate reactive oxygen species (ROS)-mediated lipid peroxidation from 9- and 13-lipoxygenase (LOX)-dependent lipid peroxidation. Free radical-mediated membrane peroxidation was measured during H2O2-dependent cell death in leaves of catalase-deficient plants. Taking advantage of these transgenic plants, we demonstrate that, under light conditions, H2O2 plays an essential role in the execution of cell death triggered by an elicitor, cryptogein, which provokes a similar ROS-mediated lipid peroxidation. Under dark conditions, however, cell death induction by cryptogein was independent of H2O2 and accompanied by products of the 9-LOX pathway. In the hypersensitive response induced by the avirulent pathogen Pseudomonas syringae pv syringae, both 9-LOX and oxidative processes operated concurrently, with ROS-mediated lipid peroxidation prevailing in the light. Our results demonstrate, therefore, the tight interplay between H2O2 and lipid hydroperoxides and underscore the importance of light during the hypersensitive response.
This article has been cited by other articles:
|
|
|
|
 
C. C.C. Chang, I. Slesak, L. Jorda, A. Sotnikov, M. Melzer, Z. Miszalski, P. M. Mullineaux, J. E. Parker, B. Karpinska, and S. Karpinski
Arabidopsis Chloroplastic Glutathione Peroxidases Play a Role in Cross Talk between Photooxidative Stress and Immune Responses
Plant Physiology,
June 1, 2009;
150(2):
670 - 683.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Mueller, B. Hilbert, K. Dueckershoff, T. Roitsch, M. Krischke, M. J. Mueller, and S. Berger
General Detoxification and Stress Responses Are Mediated by Oxidized Lipids through TGA Transcription Factors in Arabidopsis
PLANT CELL,
March 1, 2008;
20(3):
768 - 785.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. J. Mur, P. Kenton, A. J. Lloyd, H. Ougham, and E. Prats
The hypersensitive response; the centenary is upon us but how much do we know?
J. Exp. Bot.,
February 1, 2008;
59(3):
501 - 520.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Fammartino, F. Cardinale, C. Gobel, L. Mene-Saffrane, J. Fournier, I. Feussner, and M.-T. Esquerre-Tugaye
Characterization of a Divinyl Ether Biosynthetic Pathway Specifically Associated with Pathogenesis in Tobacco
Plant Physiology,
January 1, 2007;
143(1):
378 - 388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Danon, N. Sanchez Coll, and K. Apel
Cryptochrome-1-dependent execution of programmed cell death induced by singlet oxygen in Arabidopsis thaliana
PNAS,
November 7, 2006;
103(45):
17036 - 17041.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. X. Andersson, M. Hamberg, O. Kourtchenko, A. Brunnstrom, K. L. McPhail, W. H. Gerwick, C. Gobel, I. Feussner, and M. Ellerstrom
Oxylipin Profiling of the Hypersensitive Response in Arabidopsis thaliana: FORMATION OF A NOVEL OXO-PHYTODIENOIC ACID-CONTAINING GALACTOLIPID, ARABIDOPSIDE E
J. Biol. Chem.,
October 20, 2006;
281(42):
31528 - 31537.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Torres, J. D.G. Jones, and J. L. Dangl
Reactive Oxygen Species Signaling in Response to Pathogens
Plant Physiology,
June 1, 2006;
141(2):
373 - 378.
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Zaninotto, S. L. Camera, A. Polverari, and M. Delledonne
Cross Talk between Reactive Nitrogen and Oxygen Species during the Hypersensitive Disease Resistance Response
Plant Physiology,
June 1, 2006;
141(2):
379 - 383.
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Van Breusegem and J. F. Dat
Reactive Oxygen Species in Plant Cell Death
Plant Physiology,
June 1, 2006;
141(2):
384 - 390.
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Zago, S. Morsa, J. F. Dat, P. Alard, A. Ferrarini, D. Inze, M. Delledonne, and F. Van Breusegem
Nitric Oxide- and Hydrogen Peroxide-Responsive Gene Regulation during Cell Death Induction in Tobacco
Plant Physiology,
June 1, 2006;
141(2):
404 - 411.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Davoine, O. Falletti, T. Douki, G. Iacazio, N. Ennar, J.-L. Montillet, and C. Triantaphylides
Adducts of Oxylipin Electrophiles to Glutathione Reflect a 13 Specificity of the Downstream Lipoxygenase Pathway in the Tobacco Hypersensitive Response
Plant Physiology,
April 1, 2006;
140(4):
1484 - 1493.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. J. Mur, T. L. W. Carver, and E. Prats
NO way to live; the various roles of nitric oxide in plant-pathogen interactions
J. Exp. Bot.,
February 1, 2006;
57(3):
489 - 505.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
