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Published on June 24, 2005; 10.1104/pp.105.062141

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Received March 2, 2005
Returned for revision April 4, 2005
Accepted April 10, 2005

In Vivo Respiratory Metabolism of Illuminated Leaves

Guillaume Tcherkez *, Gabriel Cornic , Richard Bligny , Elizabeth Gout , and Jaleh Ghashghaie

Laboratoire d'Ecophysiologie Végétale, Unité Mixte de Recherche 8079, Bât. 362, Centre scientifique d'Orsay, Université Paris XI, 91405 Orsay cedex, France
Laboratoire de Physiologie Cellulaire Végétale, Unité Mixte de Recherche 5168, Commissariat à l'Energie Atomique-Grenoble, 38054 Grenoble cedex 09, France

* Corresponding author; email: guillaume.tcherkez{at}ese.u-psud.fr.

Day respiration of illuminated C3 leaves is not well understood and particularly, the metabolic origin of the day respiratory CO2 production is poorly known. This issue was addressed in leaves of French bean (Phaseolus vulgaris) using 12C/13C stable isotope techniques on illuminated leaves fed with 13C-enriched glucose or pyruvate. The 13CO2 production in light was measured using the deviation of the photosynthetic carbon isotope discrimination induced by the decarboxylation of the 13C-enriched compounds. Using different positional 13C-enrichments, it is shown that the Krebs cycle is reduced by 95% in the light and that the pyruvate dehydrogenase reaction is much less reduced, by 27% or less. Glucose molecules are scarcely metabolized to liberate CO2 in the light, simply suggesting that they can rarely enter glycolysis. Nuclear magnetic resonance analysis confirmed this view; when leaves are fed with 13C-glucose, leaf sucrose and glucose represent nearly 90% of the leaf 13C content, demonstrating that glucose is mainly directed to sucrose synthesis. Taken together, these data indicate that several metabolic down-regulations (glycolysis, Krebs cycle) accompany the light/dark transition and emphasize the decrease of the Krebs cycle decarboxylations as a metabolic basis of the light-dependent inhibition of mitochondrial respiration.




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