Plant Physiology Preview
Published on September 23, 2005; 10.1104/pp.105.065029
Received May 3, 2005
Returned for revision July 13, 2005
Accepted July 25, 2005
Early Effects of Salinity on Water Transport in Arabidopsis Roots. Molecular and Cellular Features of Aquaporin Expression
Yann Boursiac , Sheng Chen , Doan-Trung Luu , Mathias Sorieul , Niels van den Dries , and Christophe Maurel *
Biochimie et Physiologie Moléculaire des Plantes, Agro-Montpellier/Centre National de la Recherche Scientifique/Institut National de la Recheche Agonomique/Université Montpellier 2, Unité Mixte de Recherche 5004, F-34060 Montpellier cedex 1, France
* Corresponding author; email: maurel{at}ensam.inra.fr.
Aquaporins facilitate the uptake of soil water and mediate the regulation of root hydraulic conductivity (Lpr) in response to a large variety of environmental stresses. Here, we use Arabidopsis (Arabidopsis thaliana) plants to dissect the effects of salt on both Lpr and aquaporin expression and investigate possible molecular and cellular mechanisms of aquaporin regulation in plant roots under stress. Treatment of plants by 100 mM NaCl was perceived as an osmotic stimulus and induced a rapid (half-time, 45 min) and significant (70%) decrease in Lpr, which was maintained for at least 24 h. Macroarray experiments with gene-specific tags were performed to investigate the expression of all 35 genes of the Arabidopsis aquaporin family. Transcripts from 20 individual aquaporin genes, most of which encoded members of the plasma membrane intrinsic protein (PIP) and tonoplast intrinsic protein (TIP) subfamilies, were detected in nontreated roots. All PIP and TIP aquaporin transcripts with a strong expression signal showed a 60% to 75% decrease in their abundance between 2 and 4 h following exposure to salt. The use of antipeptide antibodies that cross-reacted with isoforms of specific aquaporin subclasses revealed that the abundance of PIP1s decreased by 40% as early as 30 min after salt exposure, whereas PIP2 and TIP1 homologs showed a 20% to 40% decrease in abundance after 6 h of treatment. Expression in transgenic plants of aquaporins fused to the green fluorescent protein revealed that the subcellular localization of TIP2;1 and PIP1 and PIP2 homologs was unchanged after 45 min of exposure to salt, whereas a TIP1;1-green fluorescent protein fusion was relocalized into intracellular spherical structures tentatively identified as intravacuolar invaginations. The appearance of intracellular structures containing PIP1 and PIP2 homologs was occasionally observed after 2 h of salt treatment. In conclusion, this work shows that exposure of roots to salt induces changes in aquaporin expression at multiple levels. These changes include a coordinated transcriptional down-regulation and subcellular relocalization of both PIPs and TIPs. These mechanisms may act in concert to regulate root water transport, mostly in the long term ( 6 h).
This article has been cited by other articles:
|
|
|
|
 
H. Yamaguchi, H. Fukuoka, T. Arao, A. Ohyama, T. Nunome, K. Miyatake, and S. Negoro
Gene expression analysis in cadmium-stressed roots of a low cadmium-accumulating solanaceous plant, Solanum torvum
J. Exp. Bot.,
October 16, 2009;
(2009)
erp313v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ehlert, C. Maurel, F. Tardieu, and T. Simonneau
Aquaporin-Mediated Reduction in Maize Root Hydraulic Conductivity Impacts Cell Turgor and Leaf Elongation Even without Changing Transpiration
Plant Physiology,
June 1, 2009;
150(2):
1093 - 1104.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. K. Lee, S. K. Cho, O. Son, Z. Xu, I. Hwang, and W. T. Kim
Drought Stress-Induced Rma1H1, a RING Membrane-Anchor E3 Ubiquitin Ligase Homolog, Regulates Aquaporin Levels via Ubiquitination in Transgenic Arabidopsis Plants
PLANT CELL,
February 1, 2009;
21(2):
622 - 641.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Fournier, A. C.J. Timmers, B. J. Sieberer, A. Jauneau, M. Chabaud, and D. G. Barker
Mechanism of Infection Thread Elongation in Root Hairs of Medicago truncatula and Dynamic Interplay with Associated Rhizobial Colonization
Plant Physiology,
December 1, 2008;
148(4):
1985 - 1995.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Ma, J. Xue, Y. Li, X. Liu, F. Dai, W. Jia, Y. Luo, and J. Gao
Rh-PIP2;1, a Rose Aquaporin Gene, Is Involved in Ethylene-Regulated Petal Expansion
Plant Physiology,
October 1, 2008;
148(2):
894 - 907.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Murai-Hatano, T. Kuwagata, J. Sakurai, H. Nonami, A. Ahamed, K. Nagasuga, T. Matsunami, K. Fukushi, M. Maeshima, and M. Okada
Effect of Low Root Temperature on Hydraulic Conductivity of Rice Plants and the Possible Role of Aquaporins
Plant Cell Physiol.,
September 1, 2008;
49(9):
1294 - 1305.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Zhang, K. Ohyama, J. Boudet, Z. Chen, J. Yang, M. Zhang, T. Muranaka, C. Maurel, J.-K. Zhu, and Z. Gong
Dolichol Biosynthesis and Its Effects on the Unfolded Protein Response and Abiotic Stress Resistance in Arabidopsis
PLANT CELL,
July 1, 2008;
20(7):
1879 - 1898.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ashkenazi, G. D. Bader, A. Kuchinsky, M. Moshelion, and D. J. States
Cytoscape ESP: simple search of complex biological networks
Bioinformatics,
June 15, 2008;
24(12):
1465 - 1466.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Prak, S. Hem, J. Boudet, G. Viennois, N. Sommerer, M. Rossignol, C. Maurel, and V. Santoni
Multiple Phosphorylations in the C-terminal Tail of Plant Plasma Membrane Aquaporins: Role in Subcellular Trafficking of AtPIP2;1 in Response to Salt Stress
Mol. Cell. Proteomics,
June 1, 2008;
7(6):
1019 - 1030.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Mahdieh, A. Mostajeran, T. Horie, and M. Katsuhara
Drought Stress Alters Water Relations and Expression of PIP-Type Aquaporin Genes in Nicotiana tabacum Plants
Plant Cell Physiol.,
May 1, 2008;
49(5):
801 - 813.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Sakurai, A. Ahamed, M. Murai, M. Maeshima, and M. Uemura
Tissue and Cell-Specific Localization of Rice Aquaporins and Their Water Transport Activities
Plant Cell Physiol.,
January 1, 2008;
49(1):
30 - 39.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Marmagne, M. Ferro, T. Meinnel, C. Bruley, L. Kuhn, J. Garin, H. Barbier-Brygoo, and G. Ephritikhine
A High Content in Lipid-modified Peripheral Proteins and Integral Receptor Kinases Features in the Arabidopsis Plasma Membrane Proteome
Mol. Cell. Proteomics,
November 1, 2007;
6(11):
1980 - 1996.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Higaki, T. Goh, T. Hayashi, N. Kutsuna, Y. Kadota, S. Hasezawa, T. Sano, and K. Kuchitsu
Elicitor-Induced Cytoskeletal Rearrangement Relates to Vacuolar Dynamics and Execution of Cell Death: In Vivo Imaging of Hypersensitive Cell Death in Tobacco BY-2 Cells
Plant Cell Physiol.,
October 1, 2007;
48(10):
1414 - 1425.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Desprez, M. Juraniec, E. F. Crowell, H. Jouy, Z. Pochylova, F. Parcy, H. Hofte, M. Gonneau, and S. Vernhettes
Organization of cellulose synthase complexes involved in primary cell wall synthesis in Arabidopsis thaliana
PNAS,
September 25, 2007;
104(39):
15572 - 15577.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Y. Jang, J. Y. Rhee, D. G. Kim, G. C. Chung, J. H. Lee, and H. Kang
Ectopic Expression of a Foreign Aquaporin Disrupts the Natural Expression Patterns of Endogenous Aquaporin Genes and Alters Plant Responses to Different Stress Conditions
Plant Cell Physiol.,
September 1, 2007;
48(9):
1331 - 1339.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wirth, F. Chopin, V. Santoni, G. Viennois, P. Tillard, A. Krapp, L. Lejay, F. Daniel-Vedele, and A. Gojon
Regulation of Root Nitrate Uptake at the NRT2.1 Protein Level in Arabidopsis thaliana
J. Biol. Chem.,
August 10, 2007;
282(32):
23541 - 23552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Martinoia, M. Maeshima, and H. E. Neuhaus
Vacuolar transporters and their essential role in plant metabolism
J. Exp. Bot.,
January 1, 2007;
58(1):
83 - 102.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Cochard, J.-S. Venisse, T. S. Barigah, N. Brunel, S. Herbette, A. Guilliot, M. T. Tyree, and S. Sakr
Putative Role of Aquaporins in Variable Hydraulic Conductance of Leaves in Response to Light
Plant Physiology,
January 1, 2007;
143(1):
122 - 133.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Laloi, A.-M. Perret, L. Chatre, S. Melser, C. Cantrel, M.-N. Vaultier, A. Zachowski, K. Bathany, J.-M. Schmitter, M. Vallet, et al.
Insights into the Role of Specific Lipids in the Formation and Delivery of Lipid Microdomains to the Plasma Membrane of Plant Cells
Plant Physiology,
January 1, 2007;
143(1):
461 - 472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. AROCA, A. FERRANTE, P. VERNIERI, and M. J. CHRISPEELS
Drought, Abscisic Acid and Transpiration Rate Effects on the Regulation of PIP Aquaporin Gene Expression and Abundance in Phaseolus vulgaris Plants
Ann. Bot.,
December 1, 2006;
98(6):
1301 - 1310.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Leshem, N. Melamed-Book, O. Cagnac, G. Ronen, Y. Nishri, M. Solomon, G. Cohen, and A. Levine
Suppression of Arabidopsis vesicle-SNARE expression inhibited fusion of H2O2-containing vesicles with tonoplast and increased salt tolerance
PNAS,
November 21, 2006;
103(47):
18008 - 18013.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Vander Willigen, O. Postaire, C. Tournaire-Roux, Y. Boursiac, and C. Maurel
Expression and Inhibition of Aquaporins in Germinating Arabidopsis Seeds
Plant Cell Physiol.,
September 1, 2006;
47(9):
1241 - 1250.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
