Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on September 16, 2005; 10.1104/pp.105.065185

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
139/2/1041    most recent
pp.105.065185v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Harada, H.
Right arrow Articles by Matsuda, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harada, H.
Right arrow Articles by Matsuda, Y.
Agricola
Right arrow Articles by Harada, H.
Right arrow Articles by Matsuda, Y.

Received May 5, 2005
Returned for revision July 9, 2005
Accepted July 27, 2005

Regulation of the Expression of Intracellular {beta}-Carbonic Anhydrase in Response to CO2 and Light in the Marine Diatom Phaeodactylum tricornutum

Hisashi Harada , Daisuke Nakatsuma , Maki Ishida , and Yusuke Matsuda *

Department of Bioscience, School of Science and Technology, Kwansei-Gakuin University, Sanda, Hyogo, Japan, 669-1337

* Corresponding author; email: yusuke{at}ksc.kwansei.ac.jp.

Cells of the marine diatom Phaeodactylum tricornutum Bohlin (UTEX 642) grown in 5% CO2 were transferred to air-level CO2 in the light or dark and allowed to acclimate to air. No accumulation of the transcript of the P. tricornutum {beta}-carbonic anhydrase 1 (ptca1) was detected in 5% CO2-grown cells, but ptca1 mRNA accumulated and reached a peak after 6 h acclimation to air but decreased over the next 18 h. A similar accumulation time course was observed in cells air-acclimated in the dark, except that levels of mRNA were <50% those in the light. These results suggest that air-level [CO2] is required to trigger the transcription of ptca1 and that light affects the extent of acclimation. During acclimation to air for 120 h in the light, levels of ptca1 mRNA exhibited a periodic oscillation with a cycle of about 24 h, which, however, was not reflected in protein accumulation levels. A 5'-upstream region from the transcription-start site toward -1,292 bp of ptca1 was cloned by inverse polymerase chain reaction, and 5'-truncations were carried out on this fragment. The truncated promoter regions were fused with the {beta}-glucuronidase gene (uidA) and introduced into P. tricornutum. The promoter fragments, truncated at positions -1,292, -824, -484, -225, and -70 bp, conferred on transformants clear CO2-responsive {beta}-glucuronidase expressions. In contrast, the CO2-responsive regulation was severely impaired or completely abolished by truncations, respectively, at position -50 or -30 bp. These results indicate that critical cis-elements required for CO2-responsive transcription of ptca1 may be located between -70 and -30 bp relative to the transcription start site.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
H. Harada, K. Nakajima, K. Sakaue, and Y. Matsuda
CO2 Sensing at Ocean Surface Mediated by cAMP in a Marine Diatom
Plant Physiology, November 1, 2006; 142(3): 1318 - 1328.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2005 by the American Society of Plant Biologists