Plant Physiology Preview
Published on July 22, 2005; 10.1104/pp.105.065326
Received May 6, 2005
Returned for revision May 25, 2005
Accepted May 25, 2005
Arabidopsis ARP7 Is an Essential Actin-Related Protein Required for Normal Embryogenesis, Plant Architecture, and Floral Organ Abscission
Muthugapatti K. Kandasamy , Elizabeth C. McKinney , Roger B. Deal , and Richard B. Meagher *
Department of Genetics, Life Sciences Building, University of Georgia, Athens, Georgia 30602
* Corresponding author; email: meagher{at}uga.edu.
The actin-related proteins (ARPs) that are localized to the nucleus are present as components of various chromatin-modifying complexes involved in chromatin dynamics and transcriptional regulation. Arabidopsis (Arabidopsis thaliana) ARP7 is a constitutively expressed nuclear protein belonging to a novel plant-specific ARP class. In this study, we demonstrate a vital role for ARP7 protein in embryogenesis and plant development. Knocking out the expression of ARP7 in an arp7-1 T-DNA mutant produced morphologically aberrant, homozygous embryos that were arrested at or before the torpedo stage of development. Hence, the arp7-1 null mutation is homozygous lethal. Knocking down the expression levels of ARP7 protein with RNA interference produced viable Arabidopsis lines affected in multiple developmental pathways and induced dosage-dependent, heritable defects in plant architecture. The transgenic plants containing greatly reduced levels of ARP7 in the nucleus were severely dwarfed with small rosette leaves that are defective in cell expansion and trichome morphology. Moreover, the ARP7-deficient RNA interference plants exhibited retarded root growth, altered flower development, delayed perianth abscission, and reduced fertility. These pleiotropic phenotypic changes suggest a critical role for the Arabidopsis ARP7 protein in the regulation of various phases of plant development through chromatin-mediated, global regulation of gene expression.
This article has been cited by other articles:
|
|
|
|
 
M. K. Kandasamy, E. C. McKinney, and R. B. Meagher
A Single Vegetative Actin Isovariant Overexpressed under the Control of Multiple Regulatory Sequences Is Sufficient for Normal Arabidopsis Development
PLANT CELL,
March 1, 2009;
21(3):
701 - 718.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. McKim, G.-E. Stenvik, M. A. Butenko, W. Kristiansen, S. K. Cho, S. R. Hepworth, R. B. Aalen, and G. W. Haughn
The BLADE-ON-PETIOLE genes are essential for abscission zone formation in Arabidopsis
Development,
April 15, 2008;
135(8):
1537 - 1546.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Cai and C. C. Lashbrook
Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing Arabidopsis ZINC FINGER PROTEIN2
Plant Physiology,
March 1, 2008;
146(3):
1305 - 1321.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Butenko, G.-E. Stenvik, V. Alm, B. Saether, S. E. Patterson, and R. B. Aalen
Ethylene-dependent and -independent pathways controlling floral abscission are revealed to converge using promoter::reporter gene constructs in the ida abscission mutant
J. Exp. Bot.,
November 1, 2006;
57(14):
3627 - 3637.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G.-E. Stenvik, M. A. Butenko, B. R. Urbanowicz, J. K.C. Rose, and R. B. Aalen
Overexpression of INFLORESCENCE DEFICIENT IN ABSCISSION Activates Cell Separation in Vestigial Abscission Zones in Arabidopsis
PLANT CELL,
June 1, 2006;
18(6):
1467 - 1476.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Martin-Trillo, A. Lazaro, R. S. Poethig, C. Gomez-Mena, M. A. Pineiro, J. M. Martinez-Zapater, and J. A. Jarillo
EARLY IN SHORT DAYS 1 (ESD1) encodes ACTIN-RELATED PROTEIN 6 (AtARP6), a putative component of chromatin remodelling complexes that positively regulates FLC accumulation in Arabidopsis
Development,
April 1, 2006;
133(7):
1241 - 1252.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. B. Meagher, R. B. Deal, M. K. Kandasamy, and E. C. McKinney
Nuclear Actin-Related Proteins as Epigenetic Regulators of Development
Plant Physiology,
December 1, 2005;
139(4):
1576 - 1585.
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. C. Pawloski, R. B. Deal, E. C. McKinney, B. Burgos-Rivera, and R. B. Meagher
Inverted Repeat PCR for the Rapid Assembly of Constructs to Induce RNA Interference
Plant Cell Physiol.,
November 1, 2005;
46(11):
1872 - 1878.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
