Plant Physiology Preview
Published on August 26, 2005; 10.1104/pp.105.065912
Received May 19, 2005
Returned for revision June 23, 2005
Accepted June 29, 2005
Evidence for In Vitro Binding of Pectin Side Chains to Cellulose
Agata W. Zykwinska , Marie-Christine J. Ralet *, Catherine D. Garnier , and Jean-François J. Thibault
Institut National de la Recherche Agronomique, Unité de Recherche Biopolymères, Interactions, Assemblages, 44316 Nantes cedex 03, France
* Corresponding author; email: ralet{at}nantes.inra.fr.
Pectins of varying structures were tested for their ability to interact with cellulose in comparison to the well-known adsorption of xyloglucan. Our results reveal that sugar beet (Beta vulgaris) and potato (Solanum tuberosum) pectins, which are rich in neutral sugar side chains, can bind in vitro to cellulose. The extent of binding varies with respect to the nature and structure of the side chains. Additionally, branched arabinans (Br-Arabinans) or debranched arabinans (Deb-Arabinans; isolated from sugar beet) and galactans (isolated from potato) were shown bind to cellulose microfibrils. The adsorption of Br-Arabinan and galactan was lower than that of Deb-Arabinan. The maximum adsorption affinity of Deb-Arabinan to cellulose was comparable to that of xyloglucan. The study of sugar beet and potato alkali-treated cell walls supports the hypothesis of pectin-cellulose interaction. Natural composites enriched in arabinans or galactans and cellulose were recovered. The binding of pectins to cellulose microfibrils may be of considerable significance in the modeling of primary cell walls of plants as well as in the process of cell wall assembly.
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