Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on August 26, 2005; 10.1104/pp.105.066399

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
139/1/64    most recent
pp.105.066399v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (43)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dutilleul, C.
Right arrow Articles by Noctor, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dutilleul, C.
Right arrow Articles by Noctor, G.
Agricola
Right arrow Articles by Dutilleul, C.
Right arrow Articles by Noctor, G.

Received May 31, 2005
Returned for revision June 13, 2005
Accepted July 8, 2005

Mitochondria-Driven Changes in Leaf NAD Status Exert a Crucial Influence on the Control of Nitrate Assimilation and the Integration of Carbon and Nitrogen Metabolism

Christelle Dutilleul , Caroline Lelarge , Jean-Louis Prioul , Rosine De Paepe , Christine H. Foyer , and Graham Noctor *

Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom
Institut de Biotechnologie des Plantes, Unité Mixte de Recherche 8618, Centre National de la Recherche Scientifique, Université de Paris XI, 91405 Orsay cedex, France

* Corresponding author; email: noctor{at}ibp.u-psud.fr.

The Nicotiana sylvestris mutant, CMS, lacks the mitochondrial gene nad7 and functional complex I, and respires using low-affinity NADH (alternative) mitochondrial dehydrogenases. Here, we show that this adjustment of respiratory pathways is associated with a profound modification of foliar carbon-nitrogen balance. CMS leaves are characterized by abundant amino acids compared to either wild-type plants or CMS in which complex I function has been restored by nuclear transformation with the nad7 cDNA. The metabolite profile of CMS leaves is enriched in amino acids with low carbon/nitrogen and depleted in starch and 2-oxoglutarate. Deficiency in 2-oxoglutarate occurred despite increased citrate and malate and higher capacity of key anaplerotic enzymes, notably the mitochondrial NAD-dependent isocitrate dehydrogenase. The accumulation of nitrogen-rich amino acids was not accompanied by increased expression of enzymes involved in nitrogen assimilation. Partitioning of 15N-nitrate into soluble amines was enhanced in CMS leaf discs compared to wild-type discs, especially in the dark. Analysis of pyridine nucleotides showed that both NAD and NADH were increased by 2-fold in CMS leaves. The growth retardation of CMS relative to the wild type was highly dependent on photoperiod, but at all photoperiod regimes the link between high contents of amino acids and NADH was observed. Together, the data provide strong evidence that (1) NADH availability is a critical factor in influencing the rate of nitrate assimilation and that (2) NAD status plays a crucial role in coordinating ammonia assimilation with the anaplerotic production of carbon skeletons.




This article has been cited by other articles:


Home page
 J Exp BotHome page
A. Galle, I. Florez-Sarasa, A. Thameur, R. De Paepe, J. Flexas, and M. Ribas-Carbo
Effects of drought stress and subsequent rewatering on photosynthetic and respiratory pathways in Nicotiana sylvestris wild type and the mitochondrial complex I-deficient CMSII mutant
J. Exp. Bot., November 20, 2009; (2009) erp344v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Ishikawa, T. Ogawa, E. Hirosue, Y. Nakayama, K. Harada, E. Fukusaki, K. Yoshimura, and S. Shigeoka
Modulation of the Poly(ADP-ribosyl)ation Reaction via the Arabidopsis ADP-Ribose/NADH Pyrophosphohydrolase, AtNUDX7, Is Involved in the Response to Oxidative Stress
Plant Physiology, October 1, 2009; 151(2): 741 - 754.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. H. Meyer, T. Tomaz, A. J. Carroll, G. Estavillo, E. Delannoy, S. K. Tanz, I. D. Small, B. J. Pogson, and A. H. Millar
Remodeled Respiration in ndufs4 with Low Phosphorylation Efficiency Suppresses Arabidopsis Germination and Growth and Alters Control of Metabolism at Night
Plant Physiology, October 1, 2009; 151(2): 603 - 619.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. L. Araujo, A. Nunes-Nesi, S. Trenkamp, V. I. Bunik, and A. R. Fernie
Inhibition of 2-Oxoglutarate Dehydrogenase in Potato Tuber Suggests the Enzyme Is Limiting for Respiration and Confirms Its Importance in Nitrogen Assimilation
Plant Physiology, December 1, 2008; 148(4): 1782 - 1796.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Garmier, A. J. Carroll, E. Delannoy, C. Vallet, D. A. Day, I. D. Small, and A. H. Millar
Complex I Dysfunction Redirects Cellular and Mitochondrial Metabolism in Arabidopsis
Plant Physiology, November 1, 2008; 148(3): 1324 - 1341.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. H.M. Schippers, A. Nunes-Nesi, R. Apetrei, J. Hille, A. R. Fernie, and P. P. Dijkwel
The Arabidopsis onset of leaf death5 Mutation of Quinolinate Synthase Affects Nicotinamide Adenine Dinucleotide Biosynthesis and Causes Early Ageing
PLANT CELL, October 1, 2008; 20(10): 2909 - 2925.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Giraud, L. H.M. Ho, R. Clifton, A. Carroll, G. Estavillo, Y.-F. Tan, K. A. Howell, A. Ivanova, B. J. Pogson, A. H. Millar, et al.
The Absence of ALTERNATIVE OXIDASE1a in Arabidopsis Results in Acute Sensitivity to Combined Light and Drought Stress
Plant Physiology, June 1, 2008; 147(2): 595 - 610.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y.-J. Liu, F. E. B. Norberg, A. Szilagyi, R. De Paepe, H.-E. Akerlund, and A. G. Rasmusson
The Mitochondrial External NADPH Dehydrogenase Modulates the Leaf NADPH/NADP+ Ratio in Transgenic Nicotiana sylvestris
Plant Cell Physiol., February 1, 2008; 49(2): 251 - 263.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Tcherkez, R. Bligny, E. Gout, A. Mahe, M. Hodges, and G. Cornic
Respiratory metabolism of illuminated leaves depends on CO2 and O2 conditions
PNAS, January 15, 2008; 105(2): 797 - 802.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Studart-Guimaraes, A. Fait, A. Nunes-Nesi, F. Carrari, B. Usadel, and A. R. Fernie
Reduced Expression of Succinyl-Coenzyme A Ligase Can Be Compensated for by Up-Regulation of the {gamma}-Aminobutyrate Shunt in Illuminated Tomato Leaves
Plant Physiology, November 1, 2007; 145(3): 626 - 639.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. F. de Longevialle, E. H. Meyer, C. Andres, N. L. Taylor, C. Lurin, A. H. Millar, and I. D. Small
The Pentatricopeptide Repeat Gene OTP43 Is Required for trans-Splicing of the Mitochondrial nad1 Intron 1 in Arabidopsis thaliana
PLANT CELL, October 1, 2007; 19(10): 3256 - 3265.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. G. Forde and P. J. Lea
Glutamate in plants: metabolism, regulation, and signalling
J. Exp. Bot., July 1, 2007; 58(9): 2339 - 2358.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
G. Vidal, M. Ribas-Carbo, M. Garmier, G. Dubertret, A. G. Rasmusson, C. Mathieu, C. H. Foyer, and R. De Paepe
Lack of Respiratory Chain Complex I Impairs Alternative Oxidase Engagement and Modulates Redox Signaling during Elicitor-Induced Cell Death in Tobacco
PLANT CELL, February 1, 2007; 19(2): 640 - 655.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P Priault, G Tcherkez, G Cornic, R De Paepe, R Naik, J Ghashghaie, and P Streb
The lack of mitochondrial complex I in a CMSII mutant of Nicotiana sylvestris increases photorespiration through an increased internal resistance to CO2 diffusion
J. Exp. Bot., September 1, 2006; 57(12): 3195 - 3207.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P Priault, C Fresneau, G Noctor, R De Paepe, G Cornic, and P Streb
The mitochondrial CMSII mutation of Nicotiana sylvestris impairs adjustment of photosynthetic carbon assimilation to higher growth irradiance
J. Exp. Bot., June 1, 2006; 57(9): 2075 - 2085.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. M. Rhoads, A. L. Umbach, C. C. Subbaiah, and J. N. Siedow
Mitochondrial Reactive Oxygen Species. Contribution to Oxidative Stress and Interorganellar Signaling
Plant Physiology, June 1, 2006; 141(2): 357 - 366.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Kolbe, S. N. Oliver, A. R. Fernie, M. Stitt, J. T. van Dongen, and P. Geigenberger
Combined Transcript and Metabolite Profiling of Arabidopsis Leaves Reveals Fundamental Effects of the Thiol-Disulfide Status on Plant Metabolism
Plant Physiology, June 1, 2006; 141(2): 412 - 422.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. Noctor, G. Queval, and B. Gakiere
NAD(P) synthesis and pyridine nucleotide cycling in plants and their potential importance in stress conditions
J. Exp. Bot., May 1, 2006; 57(8): 1603 - 1620.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
W. Shen, Y. Wei, M. Dauk, Y. Tan, D. C. Taylor, G. Selvaraj, and J. Zou
Involvement of a Glycerol-3-Phosphate Dehydrogenase in Modulating the NADH/NAD+ Ratio Provides Evidence of a Mitochondrial Glycerol-3-Phosphate Shuttle in Arabidopsis
PLANT CELL, February 1, 2006; 18(2): 422 - 441.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2005 by the American Society of Plant Biologists