Plant Physiology Preview
Published on November 18, 2005; 10.1104/pp.105.067314
Received June 20, 2005
Returned for revision September 30, 2005
Accepted October 3, 2005
Analysis of the Female Gametophyte Transcriptome of Arabidopsis by Comparative Expression Profiling
Hee-Ju Yu , Pat Hogan , and Venkatesan Sundaresan *
Section of Plant Biology, University of California, Davis, California 95616; National Horticultural Research Institute, Rural Development Administration, Suwon 440-706, Korea
Section of Plant Biology, University of California, Davis, California 95616
Section of Plant Biology, University of California, Davis, California 95616; Department of Plant Sciences, University of California, Davis, California 95616
* Corresponding author; email: sundar{at}ucdavis.edu.
The extensive data on the transcription of the plant genome are derived primarily from the sporophytic generation. There currently is little information on genes that are expressed during female gametophyte development in angiosperms, and it is not known whether the female gametophyte transcriptome contains a major set of genes that are not expressed in the sporophyte or whether it is primarily a subset of the sporophytic transcriptome. Because the embryo sac is embedded within the maternal ovule tissue, we have utilized the Arabidopsis (Arabidopsis thaliana) mutant sporocyteless that produces ovules without embryo sacs, together with the ATH1 Arabidopsis whole-genome oligonucleotide array, to identify genes that are preferentially or specifically expressed in female gametophyte development. From analysis of the datasets, 225 genes are identified as female gametophyte genes, likely a lower limit as stringent criteria were used for the analysis, eliminating many low expressed genes. Nearly 45% of the identified genes were not previously detected by sporophytic expression profiling, suggesting that the embryo sac transcriptome may contain a significant fraction of transcripts restricted to the gametophyte. Validation of six candidate genes was performed using promoter:: -glucuronidase fusions, and all of these showed embryo sac-specific expression in the ovule. The unfiltered expression data from this study can be used to evaluate the possibility of female gametophytic expression for any gene in the ATH1 array, and contribute to identification of the functions of the component of the Arabidopsis genome not represented in studies of sporophytic expression and function.
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