Plant Physiology Preview
Published on August 19, 2005; 10.1104/pp.105.067371
Received June 20, 2005
Returned for revision July 11, 2005
Accepted July 14, 2005
An Auxilin-Like J-Domain Protein, JAC1, Regulates Phototropin-Mediated Chloroplast Movement in Arabidopsis
Noriyuki Suetsugu , Takatoshi Kagawa , and Masamitsu Wada *
Division of Photobiology, National Institute for Basic Biology, Okazaki 444-8585, Japan; Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Tokyo 192-0397, Japan
Division of Photobiology, National Institute for Basic Biology, Okazaki 444-8585, Japan; Solution Oriented Research for Science and Technology, Japan Science and Technology Corporation, Kawaguchi-city, Saitama 332-0012, Japan
* Corresponding author; email: wada{at}nibb.ac.jp.
The ambient-light conditions mediate chloroplast relocation in plant cells. Under the low-light conditions, chloroplasts accumulate in the light (accumulation response), while under the high-light conditions, they avoid the light (avoidance response). In Arabidopsis (Arabidopsis thaliana), the accumulation response is mediated by two blue-light receptors, termed phototropins (phot1 and phot2) that act redundantly, and the avoidance response is mediated by phot2 alone. A mutant, J-domain protein required for chloroplast accumulation response 1 (jac1), lacks the accumulation response under weak blue light but shows a normal avoidance response under strong blue light. In dark-adapted wild-type cells, chloroplasts accumulate on the bottom of cells. Both the jac1 and phot2 mutants are defective in this chloroplast movement in darkness. Positional cloning of JAC1 reveals that this gene encodes a J-domain protein, resembling clathrin-uncoating factor auxilin at its C terminus. The amounts of JAC1 transcripts and JAC1 proteins are not regulated by light and by phototropins. A green fluorescent protein-JAC1 fusion protein showed a similar localization pattern to green fluorescent protein alone in a transient expression assay using Arabidopsis mesophyll cells and onion (Allium cepa) epidermal cells, suggesting that the JAC1 protein may be a soluble cytosolic protein. Together, these results suggest that JAC1 is an essential component of phototropin-mediated chloroplast movement.
This article has been cited by other articles:
|
|
|
|
 
Md. S. Islam, Y. Niwa, and S. Takagi
Light-Dependent Intracellular Positioning of Mitochondria in Arabidopsis thaliana Mesophyll Cells
Plant Cell Physiol.,
June 1, 2009;
50(6):
1032 - 1040.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Oikawa, A. Yamasato, S.-G. Kong, M. Kasahara, M. Nakai, F. Takahashi, Y. Ogura, T. Kagawa, and M. Wada
Chloroplast Outer Envelope Protein CHUP1 Is Essential for Chloroplast Anchorage to the Plasma Membrane and Chloroplast Movement
Plant Physiology,
October 1, 2008;
148(2):
829 - 842.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Koniger, J. A. Delamaide, E. D. Marlow, and G. C. Harris
Arabidopsis thaliana leaves with altered chloroplast numbers and chloroplast movement exhibit impaired adjustments to both low and high light
J. Exp. Bot.,
June 1, 2008;
59(9):
2285 - 2297.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Sullivan, C. E. Thomson, D. J. Lamont, M. A. Jones, and J. M. Christie
In Vivo Phosphorylation Site Mapping and Functional Characterization of Arabidopsis Phototropin 1
Mol Plant,
January 1, 2008;
1(1):
178 - 194.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Iwabuchi, T. Sakai, and S. Takagi
Blue Light-Dependent Nuclear Positioning in Arabidopsis thaliana Leaf Cells
Plant Cell Physiol.,
September 1, 2007;
48(9):
1291 - 1298.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Tsuboi, N. Suetsugu, H. Kawai-Toyooka, and M. Wada
Phototropins and Neochrome1 Mediate Nuclear Movement in the Fern Adiantum capillus-veneris
Plant Cell Physiol.,
June 1, 2007;
48(6):
892 - 896.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Ezaki, H. Kiyohara, H. Matsumoto, and S. Nakashima
Overexpression of an auxilin-like gene (F9E10.5) can suppress Al uptake in roots of Arabidopsis
J. Exp. Bot.,
February 1, 2007;
58(3):
497 - 506.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. R. Luesse, S. L. DeBlasio, and R. P. Hangarter
Plastid Movement Impaired 2, a New Gene Involved in Normal Blue-Light-Induced Chloroplast Movements in Arabidopsis
Plant Physiology,
August 1, 2006;
141(4):
1328 - 1337.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
