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Published on October 21, 2005; 10.1104/pp.105.067561

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Received June 23, 2005
Returned for revision August 11, 2005
Accepted August 19, 2005

Differential Capacity for High-Affinity Manganese Uptake Contributes to Differences between Barley Genotypes in Tolerance to Low Manganese Availability

Pai Pedas , Christopher A. Hebbern , Jan K. Schjoerring , Peter E. Holm , and Søren Husted *

Plant and Soil Science Laboratory, Royal Veterinary and Agricultural University, DK-1871 Frederiksberg C, Copenhagen, Denmark
Department of Natural Sciences, Royal Veterinary and Agricultural University, DK-1871 Frederiksberg C, Copenhagen, Denmark

* Corresponding author; email: shu{at}kvl.dk.

There is considerable variability among barley (Hordeum vulgare) genotypes in their ability to grow in soils containing a low level of plant available manganese (Mn). The physiological basis for the tolerance to low Mn availability is unknown. In this work, Mn2+ influx and compartmentation in roots of the Mn-efficient genotype Vanessa and the Mn-inefficient genotype Antonia were investigated. Two separate Mn transport systems, mediating high-affinity Mn2+ influx at concentrations up to 130 nM and low-affinity Mn2+ influx at higher concentrations, were identified in both genotypes. The two genotypes differed only in high-affinity kinetics with the Mn-efficient genotype Vanessa having almost 4 times higher Vmax than the inefficient Antonia, but similar Km values. Online inductively coupled plasma-mass spectrometry measurements verified that the observed differences in high-affinity influx resulted in a higher Mn net uptake of Vanessa compared to Antonia. Further evidence for the importance of the differences in high-affinity uptake kinetics for Mn acquisition was obtained in a hydroponic system with mixed cultivation of the two genotypes at a continuously low Mn concentration (10-50 nM) similar to that occurring in soil solution. Under these conditions, Vanessa had a competitive advantage and contained 55% to 75% more Mn in the shoots than did Antonia. Subcellular compartmentation analysis of roots based on 54Mn2+ efflux established that up to 93% and 83% of all Mn was present in the vacuole in Vanessa and Antonia, respectively. It is concluded that differential capacity for high-affinity Mn influx contributes to differences between barley genotypes in Mn efficiency.




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