Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on November 11, 2005; 10.1104/pp.105.069013

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
139/4/2006    most recent
pp.105.069013v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bentolila, S.
Right arrow Articles by Hanson, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bentolila, S.
Right arrow Articles by Hanson, M. R.
Agricola
Right arrow Articles by Bentolila, S.
Right arrow Articles by Hanson, M. R.

Received July 27, 2005
Returned for revision September 13, 2005
Accepted September 23, 2005

Ecotype Allelic Variation in C-to-U Editing Extent of a Mitochondrial Transcript Identifies RNA-Editing Quantitative Trait Loci in Arabidopsis

Stéphane Bentolila , Anne-Laure Chateigner-Boutin , and Maureen R. Hanson *

Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853

* Corresponding author; email: mrh5{at}cornell.edu.

In higher plants, RNA editing is a posttranscriptional process that converts C to U in organelle mRNAs. Although RNA editing in mitochondria occurs much more frequently than in chloroplasts, editing of exogenously supplied RNA substrates in vitro and in organello has shown that editing in the two organelles shares some common features. In particular, the 20 nucleotides upstream of the editing site play an important role in specifying the C to be edited. Biochemical approaches have allowed the identification of features of cis-sequences necessary for RNA editing to occur, but have failed to identify any of the components of the mitochondrial editing machinery. In order to implement a genetic approach for identification of editing factors, we have identified a polymorphism in the editing efficiency of a mitochondrial site between two ecotypes of Arabidopsis (Arabidopsis thaliana), Columbia (Col) and Landsberg erecta (Ler). In rosette leaves, an editing site within the ccb206 mitochondrial gene is more highly edited in Col than in Ler. Depending on the development stage and tissue analyzed, the difference in editing extent varies between the two ecotypes; for example, in floral buds, editing extent does not differ. Single-point regression analysis of the editing efficiency in a sample of recombinant inbred lines derived from a cross between Col and Ler allowed the identification of two quantitative trait loci controlling this trait. A member of the pentatricopeptide repeat protein family that carries a putative mitochondrial transit sequence has been identified near a major quantitative trait locus on chromosome 4.




This article has been cited by other articles:


Home page
 RNAHome page
J. C. Robbins, W. P. Heller, and M. R. Hanson
A comparative genomics approach identifies a PPR-DYW protein that is essential for C-to-U editing of the Arabidopsis chloroplast accD transcript
RNA, June 1, 2009; 15(6): 1142 - 1153.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Forner, A. Holzle, C. Jonietz, S. Thuss, M. Schwarzlander, B. Weber, R. C. Meyer, and S. Binder
Mitochondrial mRNA Polymorphisms in Different Arabidopsis Accessions
Plant Physiology, October 1, 2008; 148(2): 1106 - 1116.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
M. Rudinger, M. Polsakiewicz, and V. Knoop
Organellar RNA Editing and Plant-Specific Extensions of Pentatricopeptide Repeat Proteins in Jungermanniid but not in Marchantiid Liverworts
Mol. Biol. Evol., July 1, 2008; 25(7): 1405 - 1414.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. Bentolila, L. E. Elliott, and M. R. Hanson
Genetic Architecture of Mitochondrial Editing in Arabidopsis thaliana
Genetics, March 1, 2008; 178(3): 1693 - 1708.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A.-L. Chateigner-Boutin and I. Small
A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons
Nucleic Acids Res., September 27, 2007; 35(17): e114 - e114.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2005 by the American Society of Plant Biologists