Plant Physiology Preview
Published on January 13, 2006; 10.1104/pp.105.071282
Received September 12, 2005
Returned for revision October 20, 2005
Accepted December 19, 2005
"GCR1, GPA1 and Prephenate dehydratase1 are required for blue light-induced production of phenylalanine in etiolated Arabidopsis"
Katherine Mary Warpeha , Syed Salman Lateef , Yevgeniya Lapik , Marybeth Anderson , Bao-Shiang Lee , and Lon Seth Kaufman *
Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois, 60607 U.S.A
Protein Research Laboratory, Research Resources Center, University of Illinois at Chicago, Chicago, Illinois, 60612 U.S.A
Department of Biochemistry and Genetics, University of Illinois at Chicago, Chicago, Illinois, 60607 U.S.A
* Corresponding author; email: lkaufman{at}uic.edu.
Different classes of plant hormones and different wavelengths of light act through specific signal transduction mechanisms to coordinate higher plant development. A specific prephenate dehydratase protein (PD1) was discovered to have a strong interaction with GPA1, the sole canonical G protein G -subunit in Arabidopsis thaliana. PD1 is a protein located in the cytosol, present in etiolated seedlings, with a specific role in blue light-mediated synthesis of phenylpyruvate and subsequently, of phenylalanine. Insertion mutagenesis confirms that GPA1 and GCR1 (the sole canonical G protein coupled receptor in Arabidopsis) also have a role in this blue light-mediated event. In vitro analyses indicate that the increase in PD1 activity is the direct and specific consequence of its interaction with activated GPA1. Because of their shared role in the light-mediated synthesis of phenylpyruvate and phenylalanine, because they are iteratively interactive, and, because activated GPA1 is directly responsible for the activation of PD1; GCR1, GPA1 and PD1 form all of or part of a signal transduction mechanism responsible for the light mediated synthesis of phenylpyruvate, phenylalanine, and those metabolites that derive from that phenylalanine. Data are also presented to confirm that Abscisic Acid can act through the same pathway. An additional outcome of the work is the confirmation that phenylpyruvate acts as the intermediate in the synthesis of phenylalanine in etiolated plants, as it commonly does in bacteria and fungi.
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