Plant Physiology Preview
Published on November 23, 2005; 10.1104/pp.105.071589
OPEN ACCESS ARTICLE
Received September 16, 2005
Returned for revision September 16, 2005
Accepted October 13, 2005
Metabolite Profiling of Chlamydomonas reinhardtii under Nutrient Deprivation
Christian Bölling * and Oliver Fiehn
Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany
* Corresponding author; email: boelling{at}mpimp-golm.mpg.de.
A metabolite profiling technique for Chlamydomonas reinhardtii cells for multiparallel analysis of low-molecular weight polar compounds was developed. The experimental protocol was optimized to quickly inactivate enzymatic activity, achieve maximum extraction capacity, and process large sample quantities. As a result of the rapid sampling, extraction, and analysis by gas chromatography coupled to time-of-flight mass spectrometry, more than 800 analytes from a single sample could be measured, of which more than 100 could be identified. Analyte responses could be determined mostly with SEs less than 10%. Wild-type cells of C. reinhardtii strain CC-125 subjected to nitrogen-, phosphorus-, sulfur-, or iron-depleted growth conditions develop highly distinctive metabolite profiles. Individual metabolites undergo marked changes in their steady-state levels. Compared to control conditions, sulfur-depleted cells accumulated 4-hydroxyproline more than 50-fold, whereas the amount of 2-ketovaline was reduced to 2% of control levels. The contribution of each compound to the differences observed in the metabolic phenotypes is summarized in a quantitatively rigorous way by principal component analysis, which clearly discriminates the cells from different growth regimes and indicates that phosphorus-depleted conditions induce a deficiency syndrome quite different from the response to nitrogen, sulfur, or iron starvation.
This article has been cited by other articles:
|
|
|
|
 
J. L. Moseley, D. Gonzalez-Ballester, W. Pootakham, S. Bailey, and A. R. Grossman
Genetic Interactions Between Regulators of Chlamydomonas Phosphorus and Sulfur Deprivation Responses
Genetics,
March 1, 2009;
181(3):
889 - 905.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. May, S. Wienkoop, S. Kempa, B. Usadel, N. Christian, J. Rupprecht, J. Weiss, L. Recuenco-Munoz, O. Ebenhoh, W. Weckwerth, et al.
Metabolomics- and Proteomics-Assisted Genome Annotation and Analysis of the Draft Metabolic Network of Chlamydomonas reinhardtii
Genetics,
May 1, 2008;
179(1):
157 - 166.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Y. Huang, U. Roessner, I. Eickmeier, Y. Genc, D. L. Callahan, N. Shirley, P. Langridge, and A. Bacic
Metabolite Profiling Reveals Distinct Changes in Carbon and Nitrogen Metabolism in Phosphate-Deficient Barley Plants (Hordeum vulgare L.)
Plant Cell Physiol.,
May 1, 2008;
49(5):
691 - 703.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Okazaki, N. Oka, T. Shinano, M. Osaki, and M. Takebe
Differences in the Metabolite Profiles of Spinach (Spinacia oleracea L.) Leaf in Different Concentrations of Nitrate in the Culture Solution
Plant Cell Physiol.,
February 1, 2008;
49(2):
170 - 177.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Thornton, S. M. Osborne, E. Paterson, and P. Cash
A proteomic and targeted metabolomic approach to investigate change in Lolium perenne roots when challenged with glycine
J. Exp. Bot.,
May 1, 2007;
58(7):
1581 - 1590.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Brauer, J. Yuan, B. D. Bennett, W. Lu, E. Kimball, D. Botstein, and J. D. Rabinowitz
Conservation of the metabolomic response to starvation across two divergent microbes
PNAS,
December 19, 2006;
103(51):
19302 - 19307.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
